Identification of Sarcocystis capracanis in cerebrospinal fluid from sheep with neurological disease

Formisano, Pietro; Aldridge, Brian; Alony, Y; Beekhuis, Lies; Davies, Emma; Pozo, Jorge del; Dunn, Kathleen; English, Kate; Morrison, Linda; Sargison, Neil; Seguino, Alessandro; Summers, B. A.; Wilson, DC; Milne, Elspeth; Beard, Philippa M.

doi:10.1016/j.vetpar.2012.12.016

Cited by 25 publications

(16 citation statements)

References 16 publications

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“…The S. capracanis sequence amplified herein (GenBank accession no: ) showed 99% identity with S. capracanis sequence (GenBank accession no: L76472). Although, goats are recognized as intermediate hosts of S. capracanis, this finding is not surprising as this species was previously reported in the cerebrospinal fluid of two sheep, suggesting their role as another intermediate host (Formisano et al., ). For goats, only S. capracanis was identified in our study, which emphasizing its distribution as the most prevalent Sarcocystis species in Tunisia.…”

Section: Discussionsupporting

confidence: 57%

First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

Amairia

Amdouni

Rouatbi

et al. 2017

Transbound Emerg Dis

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Sarcocystosis is a parasitic disease caused by varying Sarcocystis species infecting humans and animals. It is commonly found in small ruminants causing pathogenic effects. This contributes to detrimental economic loss for local farmers and the local economy due this disease. Although the distribution of Sarcocystis can be found all over the world, the species infecting small ruminants in Tunisia is still unknown. Through this study, we aim to estimate the molecular prevalence of natural infection with Sarcocystis spp. in sheep and goats using molecular identification. Also, phylogenetic analyses were used to identify the different species of this parasite infecting small ruminants in northern Tunisia for the first time. DNA was extracted from 198 and 121, sheep and goats meat samples, respectively. The molecular prevalence of Sarcocystis spp. in sheep and goats was 58.6% (116/198) and 50.4% (61/121), respectively. Compared to the Noire de Thibar and cross-breeds, the Barbarine sheep had the highest infection prevalence (63.4%) (p = .004). Five of the 116 positive samples were sequenced identifying Sarcocystis tenella from sheep. For goats, the sequencing results showed that five positive PCR products belonged to Sarcocystis capracanis species.

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Section: Discussionsupporting

confidence: 57%

First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

Amairia

Amdouni

Rouatbi

et al. 2017

Transbound Emerg Dis

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“…The high identity (99.0%) between 18S rRNA sequences for S . tenella and S. capracanis has even led to a debate whether sheep and goat can harbor the same Sarcocystis species [ 3 , 5 ]. Blast search suggested that no sequences in GenBank had significant similarity with the ITS-1 regions of S .…”

Section: Discussionmentioning

confidence: 99%

Sarcocystis spp. in domestic sheep in Kunming City, China: prevalence, morphology, and molecular characteristics

Huang

Wen

et al. 2017

Parasite

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Sheep (Ovis aries) are intermediate hosts for at least six named species of Sarcocystis: S. tenella, S. arieticanis, S. gigantea, S. medusiformis, S. mihoensis, and S. microps. Here, only two species, S. tenella and S. arieticanis, were found in 79 of 86 sheep (91.9%) in Kunming, China, based on their morphological characteristics. Four genetic markers, i.e., 18S rRNA gene, 28S rRNA gene, mitochondrial cox1 gene, and ITS-1 region, were sequenced and characterized for the two species of Sarcocystis. Sequences of the three former markers for S. tenella shared high identities with those of S. capracanis in goats, i.e., 99.0%, 98.3%, and 93.6%, respectively; the same three marker sequences of S. arieticanis shared high identities with those of S. hircicanis in goats, i.e., 98.5%, 96.5%, and 92.5%, respectively. No sequences in GenBank were found to significantly resemble the ITS-1 regions of S. tenella and S. arieticanis. Identities of the four genetic markers for S. tenella and S. arieticanis were 96.3%, 95.4%, 82.5%, and 66.2%, respectively.

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“…Sarcocystis merozoites have also been identified in CSF samples from sheep with neurological signs (Formisano and others 2013).…”

Section: Cerebrospinal Fluid Samplingmentioning

confidence: 99%

Diagnosing limb paresis and paralysis in sheep

Crilly¹,

Rzechorzek²,

Scott³

2015

In Practice

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Paresis and paralysis are uncommon problems in sheep but are likely to prompt farmers to seek veterinary advice. A thorough and logical approach can aid in determining the cause of the problem and highlighting the benefit of veterinary involvement. While this may not necessarily alter the prognosis for an individual animal, it can help in formulating preventive measures and avoid the costs – both in economic and in welfare terms – of misdirected treatment. Distinguishing between central and peripheral lesions is most important, as the relative prognoses are markedly different, and this can often be achieved with minimal equipment. This article describes an approach to performing a neurological examination of the ovine trunk and limbs, the ancillary tests available and the common and important causes of paresis and paralysis in sheep.

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Identification of Sarcocystis capracanis in cerebrospinal fluid from sheep with neurological disease

Cited by 25 publications

References 16 publications

First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

First detection and molecular identification of Sarcocystis spp. in small ruminants in North-West Tunisia

Sarcocystis spp. in domestic sheep in Kunming City, China: prevalence, morphology, and molecular characteristics

Diagnosing limb paresis and paralysis in sheep

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