Identification of selected respiratory pathogens in endodontic infections

Nandakumar, Renu; Whiting, Justin G.; Fouad, Ashraf F.

doi:10.1016/j.tripleo.2008.01.036

Cited by 11 publications

(10 citation statements)

References 35 publications

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“…In this regard, it should be noted that we compared the LAMP assay with a real-time PCR with TaqMan probes. In fact other studies reported LOD values for PCR around 50 cp/rx (Nandakumar et al, 2008) and 22 cp/rx (Sheppard, 2004), which are in line with the LOD of 20 cp/rx obtained for the reference PCR of our study.…”

Section: Discussionsupporting

confidence: 93%

Validation of a Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Invasive Pneumococcal Disease

Paz

Brotons

Esteva

et al. 2020

Front. Cell. Infect. Microbiol.

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Current molecular PCR-based techniques used for detecting Streptococcus pneumoniae, the causative pathogen of invasive pneumococcal disease (IPD), are accurate but have a run time of several hours. We aimed to develop and validate a novel real-time loop mediated amplification (LAMP) assay for rapid detection of pneumococcus in normally sterile samples with accuracy comparable to a gold standard real-time PCR. Conserved regions of lytA were used for the design of the LAMP test. Analytical validation included assessment of linearity, limit of detection (LOD), intra-assay and inter-assay precision and analytical specificity, which was evaluated by using reference strain S. pneumoniae R6 and a quality control panel. Clinical performance was assessed on all samples collected from children with suspicion of IPD attended in Hospital Sant Joan de Deu (Barcelona, Spain) during the period April-September 2015. Fresh samples were analyzed after DNA extraction. The following values of analytical parameters were determined: linearity within the range 10 8-10 4 copies/mL; limit of detection, 5•10 3 copies/mL; intra-and inter-assay precision measured by mean coefficient of variance, 3.61 and 6.59%; analytical specificity, 9/9 pathogens similar to S. pneumoniae and 14/14 strains of different S. pneumoniae serotypes correctly identified as negative and positive results, respectively. Diagnostic sensitivity and specificity values were 100.0 and 99.3%. Median time of DNA amplification was 15 min. The new LAMP assay showed to have similar accuracy as PCR while being 5-fold faster and could become a useful diagnostic tool for early diagnosis of IPD.

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Section: Discussionsupporting

confidence: 93%

Validation of a Loop-Mediated Isothermal Amplification Assay for Rapid Diagnosis of Invasive Pneumococcal Disease

Paz

Brotons

Esteva

et al. 2020

Front. Cell. Infect. Microbiol.

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“…It has been shown that bacterial communities within the oral cavity may be reservoirs of respiratory pathogens (Scannapieco and Rethman, 2003; Didilescu et al , 2005). Few researchers have examined endodontic infections as possible sources of those microorganisms (Chaudhry et al , 1997; Nandakumar et al , 2008). In this investigation, we sought the presence of important lung pathogens, including D. pneumosintes , Enterobacter aerogenes , Streptococcus pneumoniae , and Legionella pneumophila , in the root canal microbiota.…”

Section: Discussionmentioning

confidence: 99%

Microbiologic profile of endodontic infections from HIV− and HIV+ patients using Multiple‐Displacement Amplification and Checkerboard DNA‐DNA Hybridization

et al. 2012

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Oral Diseases (2012) 18, 558–567 Objective: To compare the microbiota of endodontic infections in necrotic pulp from HIV‐negative and HIV‐positive subjects. Materials and Methods: Root canal samples from necrotic pulp were collected from 40 HIV− and 20 HIV+ subjects. Pulps were amplified using multiple displacement amplification (MDA). Then, checkerboard DNA–DNA hybridization was employed to assess the levels of 107 microbial taxa. The percentage of DNA probe count and the percentage of teeth colonized by each test species were investigated. Significant differences between groups regarding proportions of taxa and prevalence of the test species were sought using the Mann–Whitney test and the Chi‐square analysis, respectively. Results: The most prevalent taxa detected were Dialister pneumosintes, Stenotrophomonas maltophilia, Streptococcus sobrinus, Corynebacterium diphteriae, and Helicobacter pylori among HIV‐ subjects and D. pneumosintes, Prevotella tannerae, Porphyromonas gingivalis, Parvimonas micra, Prevotella nigrescens, and Corynebacterium diphtheriae among HIV+ individuals. D. pneumosintes, C. diphtheria, and C. albicans were the most abundant species in the HIV‐ group, whereas the predominant taxa in HIV+ samples were P. tannerae, D. pneumosintes and Olsenella uli. P. tannerae, O. uli, Veilonella dispar, Bacteroides fragilis, and Actinomyces meyeri were significantly more abundant in HIV+ samples. Conclusions: There were significant differences in the prevalence and proportions of specific microbial taxa between HIV− and HIV+ individuals. The root canal microbiota may represent a reservoir of important oral and medical pathogens, mainly in HIV+ individuals.

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“…25 Bacterial species that do not play a significant role in oral infections but are important systemic pathogens that have been frequently detected in the oral environment. 26,27 In the present study we have identified two phylotypes of streptococci that had 99% sequence identity with S. pneumoniae ATCC 700669 and S. pneumoniae G54 from subgingival plaque adjacent to the infected root canals. This finding is noteworthy in view of the "oral-systemic connection" of disease pathogenesis recently postulated, where the oral flora serve as a reservoir of pathogens for a number of systemic infections such as nosocomial pneumonias.…”

Section: Discussionmentioning

confidence: 58%