Identification of selenium-containing proteins in HEK 293 kidney cells using multiple chromatographies, LC–ICPMS and nano-LC–ESIMS

Chitta, Karnakar R.; Figueroa, Julio A. Landero; Kodali, Phanichand; Caruso, Joseph A.; Merino, Edward J.

doi:10.1016/j.talanta.2013.03.003

Cited by 10 publications

(6 citation statements)

References 36 publications

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“…ESI is a soft and common ionization strategy for analytes, which supports the concurrent analysis of various constituents and unknown structures (L. Yin et al, 2019). ESIMS has been largely applied in single-cell metabolomics (Chitta et al, 2013;Hu & Yao, 2022;Mellors et al, 2010), proteomics (Lombard-Banek et al, 2016;S. Xu et al, 2020), and lipidomics (J.…”

Section: Electrospray Ionization Msmentioning

confidence: 99%

Proteomics mining of cancer hallmarks on a single‐cell resolution

Zuo

Yang

et al. 2023

Mass Spectrometry Reviews

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Dysregulated proteome is an essential contributor in carcinogenesis. Protein fluctuations fuel the progression of malignant transformation, such as uncontrolled proliferation, metastasis, and chemo/radiotherapy resistance, which severely impair therapeutic effectiveness and cause disease recurrence and eventually mortality among cancer patients. Cellular heterogeneity is widely observed in cancer and numerous cell subtypes have been characterized that greatly influence cancer progression. Population‐averaged research may not fully reveal the heterogeneity, leading to inaccurate conclusions. Thus, deep mining of the multiplex proteome at the single‐cell resolution will provide new insights into cancer biology, to develop prognostic biomarkers and treatments. Considering the recent advances in single‐cell proteomics, herein we review several novel technologies with particular focus on single‐cell mass spectrometry analysis, and summarize their advantages and practical applications in the diagnosis and treatment for cancer. Technological development in single‐cell proteomics will bring a paradigm shift in cancer detection, intervention, and therapy.

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Section: Electrospray Ionization Msmentioning

confidence: 99%

Proteomics mining of cancer hallmarks on a single‐cell resolution

Zuo

Yang

et al. 2023

Mass Spectrometry Reviews

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“…Transport efficiencies were dependent on the chemical form of Se and type of application (separate or simultaneous) with the highest values for SeMet (9.1%) and Se VI (7.9%) when adding Se chemical forms separately or simultaneously, respectively. In other study 174 Se-containing proteins were isolated from HEK 293 kidney cells incubated with 100 mM SeMet for 24 h. Proteins were screened by using a 2D chromatography method involving SEC (300 Â 7.5 mm, 10 mm) and a RP C 4 column (150 Â 0.50 mm, 5 mm). Seleniumcontaining RP fractions were digested with trypsin for iden-tication using HPLC-Chip-ES-IT-MS.…”

Section: Seleniummentioning

confidence: 99%

Atomic Spectrometry Update: review of advances in elemental speciation

Harrington

Clough

Hill

et al. 2015

J. Anal. At. Spectrom.

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“…This integrated LC microchip has the advantage of online sample enrichment prior to chromatographic separation. The Chip Cube system with a standard Agilent LC microchip has been applied for chip‐based LC‐MS analysis in various fields of bioanalytical applications including proteomics , glycomics , as well as clinical and pharmaceutical studies . In order to improve the spray and signal stability, an additional flow path for post‐column makeup flow (MUF) was integrated onto the polyimide LC microchip .…”

Section: Recent Developments In Chip‐based Instrumentationmentioning

confidence: 99%

Microfluidic chip‐based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications: An update

Lin

Fuh

2013

Electrophoresis

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Many microfluidic chip-based LC-MS systems have been utilized for high-throughput analysis in various fields of bioanalytical applications such as proteomic, glycomic, pharmaceutical, and clinical research. This review is an update of a previous review article (Electrophoresis 2012, 33, 635-643) to mainly cover the most recent advancements in chip-based LC-MS for determining small molecules in bioanalysis. First, the different types of microfluidic chip devices for chip-based LC-MS analysis will be discussed. Following the discussion of the recent developments in the chip-based instrumentation, the applications of chip-based LC-MS for determining small molecules, such as glycans, pharmaceutical drugs, drugs of abuse, drug metabolites, and biomarkers in various biological sample matrixes will also be included in this review.

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Identification of selenium-containing proteins in HEK 293 kidney cells using multiple chromatographies, LC–ICPMS and nano-LC–ESIMS

Cited by 10 publications

References 36 publications

Proteomics mining of cancer hallmarks on a single‐cell resolution

Proteomics mining of cancer hallmarks on a single‐cell resolution

Atomic Spectrometry Update: review of advances in elemental speciation

Microfluidic chip‐based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications: An update

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