Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach

Högberg, Nils; Land, Carl Johan

doi:10.1515/hf.2004.030

Cited by 20 publications

(6 citation statements)

References 35 publications

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“…Among them, B. adusta, C. lacerata, G. trabeum, and T. versicolor were predominantly isolated from different sites. The most well-known corticioid fungi in wood in service are Coniophora puteana, Phanerochaete chrysosporium, Serpula lacrymans, and Stereum sanguinolentum (Duncan and Lombard 1965;Burdall 1985;Palfreyman et al 1995;Hö gberg and Land 2004). In addition to polypore fungi, many corticioid fungi were also found in wood products in the present study.…”

Section: Isolatesupporting

confidence: 62%

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Decay fungi from playground wood products in service using 28S rDNA sequence analysis

et al. 2005

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In order to establish integrated control strategies of wood degradation, a systematic survey of basidiomycete decay fungi colonizing various wood products in service is a prerequisite to increasing the service life of wood products. As a first step, we initiated a thorough survey of basidiomycete decay fungi colonizing playground wood products. For accurate fungal identification, traditional methods were complemented with molecular methods, including a BLAST search for large-subunit 28S rDNA sequences in Genbank and phylogenetic analysis. A total of 132 basidiomycete fungi, including 30 different fungal taxa, were isolated from 35 playgrounds in 15 different areas. Eight species, Bjerkandera adusta, Ceriporia lacerata, Gloeophyllum trabeum, Peniophora sp., Phanerochaete sordida, Schizophyllum commune, Sistotrema brinkmannii, and Trametes versicolor, were predominantly isolated. A combination of traditional and molecular tools allowed a more detailed identification and diversity.

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Section: Isolatesupporting

confidence: 62%

“…The ITS region has been preferentially used in the identification of fungi on wood in service, such as houserot fungi, because of its high sequence and length variability Hö gberg and Land 2004). Furthermore, we did not attempt to sequence all of the individual isolates and other variable genes, such as the internal transcribed spacer.…”

Section: Isolatementioning

confidence: 99%

Decay fungi from playground wood products in service using 28S rDNA sequence analysis

et al. 2005

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“…(Käärik & Rennerfelt, 1957;Rayner & Boddy, 1988). Recently, PCR-RFLP methods have been employed not only to identify fungi from mycelial cultures, but also to discover and identify fungal presence directly in wood (Johannesson & Stenlid, 1999;Jasalavich et al, 2000;Adair et al, 2002;Högberg & Land, 2004). One obstacle has been to develop efficient methods for extracting amplifiable fungal DNA from wood, e.g.…”

Section: Introductionmentioning

confidence: 99%

Wood-inhabiting fungal communities in woody debris of Norway spruce (Picea abies (L.) Karst.), as reflected by sporocarps, mycelial isolations and T-RFLP identification

Allmér

Vasiliauskas

Ihrmark

et al. 2006

FEMS Microbiology Ecology

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Wood-inhabiting fungi play a key role in forest ecosystems and constitute an essential part of forest biodiversity. We therefore examined the composition and abundance of wood-inhabiting fungi by three methods: sporocarp counts, mycelial culturing and direct amplification of internal transcribed spacer terminal restriction fragment length polymorphism from wood combined with sequencing of reference rDNA. Seven-year-old slash piles left after a thinning were analyzed in a 50-year-old Norway spruce plantation. Fifty-eight fungal species were detected from the piled branches and treetops. More species were revealed by sporocarp counts and cultured mycelia than by direct amplification from wood. In principle, sporocarp monitoring may reveal all fruiting taxa, but it poorly reflects their relative abundance in the wood. In contrast, terminal restriction fragment length polymorphism will record the most frequent fungal taxa in the wood, but it may overlook uncommon taxa. Culturing mycelia from wood gives a bias towards species favoured by the cultural medium. The results demonstrate the advantage and the limitations of these methods to be considered in analyses of fungal communities in wood.

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“…Therefore, identification keys were used together with microscopy (Gistl 1946;Huckfeldt and Schmidt 2015). If morphological examination did not lead to an unambiguous result as was the case in 177 samples, molecular methods (PCR, sequencing of the ITS region of ribosomal DNA) were used (Gardes and Bruns 1993;Schmidt 2000;Högberg and Land 2004). Identification based on the sequence data was carried out using BLAST (http://blast .ncbi.nlm.nih.gov) and GenBank (http:// www.ncbi.nlm.nih.gov/genba nk).…”

Section: Identification Of Fungimentioning

confidence: 99%

Distribution of building-associated wood-destroying fungi in the federal state of Styria, Austria

Haas¹,

Mayrhofer

Habib³

et al. 2019

Eur. J. Wood Prod.

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Wood is an important construction material, but when used incorrectly it can be subjected to deterioration by wood-destroying fungi. The brown rot producing dry rot fungus (Serpula lacrymans) is by far the most dangerous wood-destroying fungus in Europe. In the present publication, 645 fungal samples from damaged wood in the federal state of Styria (Austria) were examined and recorded by isolation date, geographical location, species identification of the wood-destroying fungus, location of damage, construction method, and age and type of building. In Styria, Serpula spp. accounted for 61.5% of damages, followed by Antrodia spp. (10.7%) and the genera Gloeophyllum (8.2%), Coniophora (3.9%) and Donkioporia (1.1%). Properties in the area of the Styrian capital Graz and old buildings were more often infested by wood-destroying fungi than houses in the rural area and new constructions.

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Identification of Serpula lacrymans and other decay fungi in construction timber by sequencing of ribosomal DNA – A practical approach

Cited by 20 publications

References 35 publications

Decay fungi from playground wood products in service using 28S rDNA sequence analysis

Decay fungi from playground wood products in service using 28S rDNA sequence analysis

Wood-inhabiting fungal communities in woody debris of Norway spruce (Picea abies (L.) Karst.), as reflected by sporocarps, mycelial isolations and T-RFLP identification

Distribution of building-associated wood-destroying fungi in the federal state of Styria, Austria

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