Identification of serum protein markers for early diagnosis of pregnancy in buffalo

Abstract

Improper or delayed pregnancy diagnosis has significant impact over animal production, particularly in buffaloes which inherently suffer from several reproductive inefficiencies. Thus the present study has undertaken to identify serum protein markers pertaining to early pregnancy diagnosis in buffaloes. Serum samples were collected from 10 pregnant Murrah Buffalo heifers at weekly intervals from days 0-35 post-artificial insemination and from 12 inseminated non-pregnant cyclic buffalo heifers on days 0, 7, 14 and 21. Two-dimensional gel electrophoresis and densitometric analysis revealed the presence of five protein spots showing average density fold change of ≥4 during early pregnancy. Mass spectrometry analysis identified these up-regulated proteins as anti-testosterone antibody light chain, apolipoprotein A-II precursor, serum amyloid A, cytokeratin type II, component IV isoform 1, which are have established roles in embryogenesis, but over-expression of the fifth identified protein immunoglobulin lambda light chain in pregnancy has been elucidated as a novel finding in the current study. Further, with bioinformatics analysis, potential antigenic B-cell epitopes were predicted for all these five proteins. An antibody cocktail-based approach involving antibodies against all these five up-regulated entire proteins or their epitopes could be developed for early detection of pregnancy in buffaloes. © 2016 Japanese Society of Animal Science.

Early Detection of Pregnancy in Goats Based on Protein Expression Pattern using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

“…These proteins produced by uterus and placenta have been used to detect pregnancy in many species (Lee et al, 2015). In recent years, several studies have investigated markers in serum during early pregnancy in domestic animals (Balhara et al, 2014;Buragohain et al, 2016;Buragohain et al, 2017;Gebremedhn et al, 2018). In a previous study, serum proteinograms were compared in nonpregnant and pregnant cows It was reported that the total protein, albumin, globulin, -globulin concentration were significantly different between non-pregnant and pregnant cows during the eighth month of pregnancy (Žvorc et al, 2000).…”

Erken Gebelik Teşhisi Amacıyla Sığır Gebelik İlişkili Glikoprotein (bPAGSerum ve Süt) ve Sığır Gebelik Spesifik Protein B (bPSP-B) Testlerinin Birbirileriyle ve Transrektal Ultrasonografik Bulgularla Karşılaştırılması

“…it has great importance to diagnose early pregnancy in order to increase the production volume of livestock sector. This will also lead to increased profitability and rural development [6] . bpAG produced in the ruminant trophoblast cells can be detected in the blood of pregnant cows [7] .…”

Exploration of urinary metabolite dynamicity for early detection of pregnancy in water buffaloes

“…As the estrous cycle repeats after 21 days and both the aforesaid methods are unable to detect pregnancy within 21 days of post-insemination, so escape of at least one estrous cycle in case of every unsuccessful conception stands inevitable. Further, the probability of escaping estrous cycle in buffaloes is higher than the other bovines as 15-73% of buffaloes present silent heat symptom, perticularly in the summer season [12,16]. So, the necessity of an alternate pregnancy diagnostic method in buffaloes before 21 days of postinsemination is still well-perceived and vividly justi ed the objective of the current study.…”

“…and signi cantly, the potential biomarkers predicted in the current study were also in accordance with the observation (Table 3) (Figure S1-S5). [12][13]. The most commonly employed pregnancy diagnosis methods in buffaloes include per-rectal palpation that can detect pregnancy accurately but not earlier than 32 to 35 days post-insemination; while the other method transrectal ultrasonography serves the purpose only after 25 days of insemination [14][15].…”