Identification of Seven
            <i>Treponema</i>
            Species in Health- and Disease-Associated Dental Plaque by Nested PCR

Willis, Stephanie G.; Smith, Kathryn S.; Dunn, V. L.; Gapter, L. A.; Riviere, K. H.; Riviere, George R.

doi:10.1128/jcm.37.3.867-869.1999

Cited by 80 publications

(40 citation statements)

References 13 publications

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“…Studies have included subjects of various races and ethnic groups and with diagnoses of chronic periodontitis, early onset periodontitis, and aggressive periodontitis. Evidence has come through cross-sectional studies, baseline microbial count data for longitudinal prognostic studies, and initial count data for periodontal therapy and maintenance care studies (1,3,8,136,157,161,162,176,183). The significant association between lesion severity and the total treponeme count has been impressively consistent, and those studies that have used techniques for surveying a wide spectrum of treponemal phylotypes have reported great diversity in the types of spirochetes associated with advanced lesions.…”

Section: Treponema Putidum 181mentioning

confidence: 99%

Spirochetes at the forefront of periodontal infections

Ellen¹,

Galimanas²

2005

Periodontology 2000

154

146

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Section: Treponema Putidum 181mentioning

confidence: 99%

Spirochetes at the forefront of periodontal infections

Ellen¹,

Galimanas²

2005

Periodontology 2000

154

146

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“…Proper diagnosis of the disease depends on early detection of the above bacteria. Currently, the l6S rRNA-based polymerase chain reaction (PCR) method seems to be the most sensitive and rapid method for determining the prevalence of such microorganisms (l, 6,10). Accurate quantification of periodontal pathogens in subgingival plaque is needed for understanding the etiologic role of these bacteria.…”

mentioning

confidence: 99%

Rapid Detection and Quantification of Five Periodontopathic Bacteria by Real‐Time PCR

Sakamoto

Takeuchi

Umeda

et al. 2001

Microbiology and Immunology

142

112

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Abstract:The quantity of periodontopathic bacteria in plaque samples is an important determinant for understanding the etiologic role of bacteria. The real-time PCR method was used to detect and quantify periodontopathic bacteria, such as Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Porphyromonas gingivalis, Treponema denticola, and Treponema socranskii, in saliva and subgingival plaque samples. There was good agreement between the results of conventional PCR and real-time PCR methods. Using the LighrCycler" system we were able to determine the amount of periodontopathic bacteria within an hour. The real-time PCR method was linear for samples containing from IlY to more than lOS cells (r=O.999). The application of the real-time PCR method should be useful in the rapid detection and quantification of periodontopathic bacteria in clinical samples.Key words: Periodontopathic bacteria, Periodontitis, Quantitative PCR Periodontal disease, a polymicrobial mixed infection, is one of the major oral diseases. It is caused by several microbial species, such as Actinobacillus actinomycetemcomitans, Bacteroides forsythus, Porphyromonas gingivalis, Treponema denticola, and Treponema socranskii. Proper diagnosis of the disease depends on early detection of the above bacteria. Currently, the l6S rRNA-based polymerase chain reaction (PCR) method seems to be the most sensitive and rapid method for determining the prevalence of such microorganisms (l, 6, 10). Accurate quantification of periodontal pathogens in subgingival plaque is needed for understanding the etiologic role of these bacteria. The conventional PCR (endpoint PCR) method detects the plateau phase of the reaction, however, is difficult to quantify. Recently, a real-time PCR using the Lightf'ycler" system (Roche Molecular Biochemicals, Mannheim, Germany) that allows monitoring of the exponential phase has been used to detect Borrelia burgdorferi in tissue samples (5). This method allows rapid detection and quantification of the above bacteria in clinical samples. In addition, a real-time PCR using the TaqMan system (PElABI) has been used to quantitate B. forsythus (7) givalis (3) in subgingival plaque. This system, however, requires more time for analysis than the Lightf'ycler" system.In this study, we compared three methods, the conventional PCR method, the real-time PCR method using the Lightf'ycler" system, and the culture method, for the detection and quantification of periodontopathic bacteria including A. actinomycetemcomitans, B. forsythus, P. gingivalis, T. denticola, and T. socranskii in saliva and subgingival plaque. Materials and MethodsSample collection and nucleic acid extraction. Saliva samples were collected in sterile plastic tubes from five patients [three subjects with rapidly progressive periodontitis (RP), mean age ± standard deviation (SD) of 32 ± 8.7 years, and two subjects with adult periodontitis (AP), 60±5.7 years]. The saliva samples were washed four times with sterile distilled water. After the final wash, bacterial cell p...

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“…Our original homology search revealing that Tv2483 was a homolog of Tp0309 provided initial optimism that the T. vincentii homolog could serve as a surrogate in our efforts to characterize the intractable Tp0309 protein biochemically and biophysically. Although T. vincentii is not an obligate human pathogen like T. pallidum, it often is found in cases of periodontal disease, typically in association with other oral spirochetes and anaerobes (such as Fusobacterium) (33,34). Thus, there are obvious salient biological differences between T. pallidum and T. vincentii and the human sites that they inhabit.…”

Section: Discussionmentioning

confidence: 99%

Biophysical insights into a highly selective L-arginine-binding lipoprotein of a pathogenic treponeme

Deka¹,

Liu²,

Tso

et al. 2018

Preprint

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Biophysical and biochemical studies on the lipoproteins and other periplasmic proteins from the spirochetal species Treponema pallidum have yielded numerous insights into the functioning of the organism's peculiar membrane organization, its nutritional requirements, and intermediary metabolism. However, not all T. pallidum proteins have proven to be amenable to biophysical studies. One such recalcitrant protein is Tp0309, a putative polar-amino-acid-binding protein of an ABC transporter system. To gain further information on its possible function, a homolog of the protein from the related species T. vincentii was used as a surrogate. This protein, Tv2483, was crystallized, resulting in the determination of its crystal structure at a resolution of 1.75 Å. The protein has a typical fold for a ligand-binding protein, and a single molecule of L-arginine was bound between its two lobes.Differential scanning fluorimetry and isothermal titration calorimetry experiments confirmed that L-arginine bound to the protein with unusually high selectivity.However, further comparison to Tp0309 showed differences in key amino-acid-binding residues may impart an alternate specificity for the T. pallidum protein.

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Identification of Seven Treponema Species in Health- and Disease-Associated Dental Plaque by Nested PCR

Cited by 80 publications

References 13 publications

Spirochetes at the forefront of periodontal infections

Spirochetes at the forefront of periodontal infections

Rapid Detection and Quantification of Five Periodontopathic Bacteria by Real‐Time PCR

Biophysical insights into a highly selective L-arginine-binding lipoprotein of a pathogenic treponeme

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