Identification of sex-determining loci in hybridizing Catostomus fish species

Abstract

Despite the near-universality of gonochorism (separate sexes) in eukaryotic organisms, the underlying mechanisms of sex determination are poorly understood and highly variable in some taxa. In hybridizing species, sex determination mechanisms may promote or impede reproductive isolation depending on whether mechanisms are similar between species. In Catostomus fishes, contemporary hybridization is variable and extensive. In the present study, we aim to describe the genetic basis of sex determination in bluehead (Catostomus discobolus) and white suckers (Catostomus commersonii) to understand the impact of sex determination on reproductive isolation. We used genotyping-by-sequencing genomic data from Catostomus species and their hybrids to identify regions of the genome associated with sex using a genome-wide association study and the identification of sex-specific loci. We found a genetic basis of sex determination in Catostomus fishes, with a region of the genome significantly associating with sex in bluehead suckers. This region is suggestive of a master sex-determining region in bluehead suckers but is not significant in white suckers, implying that either the sex-determining region of the genome differs in these two species that hybridize, or that sample size was insufficient to identify this genomic region in white suckers. By describing and comparing sex-determination systems across Catostomus fish species, we highlight the relationship between sex determining systems and hybridization in closely related fish species.

Genetic sex determination in three closely related hydrothermal vent gastropods, including one species with intersex individuals

“…Identifying sex chromosomes can be achieved by looking for loci that are present on only one of the sex chromosomes (Gautier, 2014;Stovall et al, 2018;Feron et al, 2021;Garcia-Raventós et al, 2023), or by looking at heterozygosity and differentiation between sexes at homologous loci (e.g. Berset-Brändli et al, 2006;Brelsford et al, 2017;Käfer et al, 2021;Hearn et al, 2022; see also Pyne et al, 2022 for an association study). Here we followed the second approach (see Palmer et al, 2019 for a review of methods).…”

Genetic sex determination in three closely related hydrothermal vent gastropods, including one species with intersex individuals

“…Identifying sex chromosomes can be achieved by looking for loci that are present on only one of the sex chromosomes (Gautier, 2014;Stovall et al, 2018;Feron et al, 2021;Garcia-Raventós et al, 2023), or by looking at heterozygosity and differentiation between sexes at homologous loci (e.g. Berset-Brändli et al, 2006;Brelsford et al, 2017;Käfer et al, 2021;Hearn et al, 2022; see also Pyne et al, 2022 for an association study). Here we followed the second approach (see Palmer et al, 2019 for a review of methods).…”