Identification of Single Nucleotide Polymorphism and Pathway Analysis of Apolipoprotein A5 (APOA5) Related to Fatty Acid Traits in Indonesian Sheep

Abstract

This study was aimed to identify single nucleotide polymorphism (SNP) and pathway analysis of APOA5 with fatty acids traits in sheep. A total of 47 rams consisted of 20 heads of Javanese Fat Tailed (JFT), 17 heads of Javanese Thin Tailed (JTT), and 10 heads of Garut Composite Sheep (GCS) were used in this study. Fatty acids traits were measured at the age of 12 months with the average body weight of 25-30 kg. Identification of polymorphism of APOA5 (g.26929941 C>T) gene were analyzed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The pathway analysis of APOA5 gene was performed using Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The SNP of APOA5 gene were found polymorphic with three genotypes (CC, CT, and TT). The gene frequency of CC, CT, and TT were 0.83, 0.11, and 0.06, respectively. The chi square test revealed that the locus of APOA5 (g.26929941 C>T) was in Hardy-Weinberg equilibrium, except in thin tailed sheep. The chi-square values of JFT, JTT, and GCS were 0.05, 0.03, and 0.04, respectively. A SNP of APOA5 was associated (P<0.05) with polyunsaturated fatty acids including eicosapentanoic acid (C20:5n3) and docosahexanoic (C22:6n3) and saturated fatty acid lauric acid (C12:0) in combined population (JFT, JTT, and GCS). Furthermore, pathway analysis showed that APOA5 belonged to phagosome and peroxisome proliferator-activated receptors (PPAR) signaling pathway. In conclusion, this analysis has identified APOA5 and related pathway crucial for fatty acid composition and metabolism in sheep, as well as this gene provide molecular marker to select sheepmeat with high unsaturated fatty acid.

Identification of Single Nucleotide Polymorphism and Association Analysis of Alpha 2-Heremans Schmid Glycoprotein (AHSG) Gene Related to Fatty Acid Traits in Sheep

“…In combined population, the most abundant saturated fatty acid in muscle was palmitic acid (C16:0) followed by stearic acid (C18:0) and myristic acid (C14:0), respectively, it is in agreement with the previous results Gunawan et al (2018)…”

“…Meat has a vital role in human diet as a source of protein, fats, minerals and essential vitamins (Wood et al 2007;Cabrera and Saadoun 2014;Quiñones et al 2017). Fatty acids are components of fats and are grouped into saturated fatty acids and unsaturated fatty acids, the latter are classified as monounsaturated fatty acids (MUFA) with one double bond and polyunsaturated fatty acids (PUFA) with the presence of more than one double bond (PUFA) (Hidayati et al 2015; Gunawan et al 2018).…”

Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep

“…The HSD17β13 gene of SNP g.101 689 913 C>T had no significant association with flavor and odor (Table 5). In Indonesian lamb quality, several genes that affected flavor odor and fatty acid composition were APOA5 (Gunawan et al, 2018), CYP2A6 (Listyarini et al, 2018), BHMT (Munyaneza et al, 2019), and CYP2E1 (Harahap et al, 2020).…”

Identifikasi Keragaman Gen DGAT1 serta Asosiasinya terhadap Karakteristik Karkas dan Sifat Perlemakan Domba

“…2004). Selain itu, sifat perlemakan yang terdiri dari konten lemak dan komposisi asam lemak perlu diarahkan terhadap asam lemak tak jenuh yang tinggi dan rendah kolesterol yang berasosiasi positif terhadap kesehatan (Gunawan et al, 2018b). Konsumsi daging yang mengandung asam lemak jenuh tinggi dapat menyebabkan peningkatan kolesterol plasma yang berimplikasi pada penyakit beberapa penyakit diantaranya kardiovaskuler (Alvarenga et al, 2015).…”