Identification of six Cytospora species on Chinese chestnut in China

Abstract

Chinese chestnut (Castanea mollissima) is an important crop tree species in China. In the present study, Cytospora specimens were collected from Chinese chestnut trees and identified using molecular data of combined ITS, LSU, ACT and RPB2 loci, as well as morphological features. As a result, two new Cytospora species and four new host records were confirmed, viz. C. kuanchengensissp. nov., C. xinglongensissp. nov., C. ceratospermopsis, C. leucostoma, C. myrtagena and C. schulzeri.

Identification and Characterization of Leaf-Inhabiting Fungi from Castanea Plantations in China

“…In China, previous studies have revealed a high fungal diversity associated with chestnut branch cankers. For example, three Coryneum species [ 8 ], two Cryphonectria species [ 2 ], six Cytospora species and seven Dendrostoma species were described from the cankered branches [ 9 , 10 ]. In addition, some cryptic species were discovered, such as Aurantiosacculus castaneae [ 2 ], Endothia chinensis [ 2 ], Melanops castaneicola and Neopseudomelanconis castaneae [ 11 , 12 ].…”

Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species

“…Nevertheless, the species occurrence may be related to geographical and environmental factors rather than host specificity (Fan et al, 2014a , b ; Fan et al, 2015a , b ). To more accurately identify Cytospora species, several species have been described based on morphological observations and multilocus phylogeny in recent studies (Yang et al, 2015 ; Lawrence et al, 2017 , 2018 ; Zhu et al, 2018 , 2020 ; Fan et al, 2020 ; Jiang et al, 2020 ; Shang et al, 2020 ). Norphanphoun et al ( 2017 ) used four loci to describe 14 new species isolated from Rosa, Salix , and Sorbus .…”

High Diversity of Cytospora Associated With Canker and Dieback of Rosaceae in China, With 10 New Species Described

“…A total of 144 strains from Iran represented 20 species of Cytospora based on ITS phylogeny ( Fotouhifar et al, 2010 ). Later, many species were described based on multilocus phylogeny in recent studies ( Fan et al, 2014a , b , 2015a , b ; Yang et al, 2015 ; Lawrence et al, 2017 , 2018 ; Zhu et al, 2018 , 2020 ; Jiang et al, 2020 ; Shang et al, 2020 ). Fan et al (2020) provided an assessment of 52 species of Cytospora in China using a six-locus phylogeny [internal transcribed spacer (ITS), large nuclear ribosomal RNA subunit (LSU), actin ( act ), RNA polymerase II subunit ( rpb2 ), translation elongation factor 1-α ( tef1- α), and beta-tubulin ( tub2 )].…”