Identification of Small Single- and Double-Stranded RNAs Associated with Severe Symptoms in Beet Western Yellows Virus-Infected<i>Capsella bursa-pastoris</i>

Falk, Bryce W.; Duffus, J. E.

doi:10.1094/phyto-74-1224

Cited by 34 publications

(26 citation statements)

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“…Selected clones to the 6 kb genomic RNA reacted with one, two or all three of these RNAs demonstrating that these smaller RNAs are subsets of the nucleotide sequence of the 6 kb RNA. The size estimates for these RNAs are similar to those reported previously for replicative form BWYV dsRNAs produced in BWYVinfected plants, and those reported for the related BYDV (Falk & Duffus, 1984;Gildow et al, 1983). They also are similar in size to the three RNAs detected in BYDV-infected tissues by Northern blot anlaysis (Gerlach et al, 1987).…”

Section: Discussionsupporting

confidence: 87%

“…We previously showed that when virion RNAs of the ST9 BWYV isolate are analysed by agarose gel electrophoresis, two prominent ssRNAs of approximately 6 kb and 3.1 kb are detected (Falk & Duffus, 1984). Eighteen of the above mentioned cDNA clones, nine of which were obtained by G-C tailing and nine by linker ligation, were nick-translated and used in hybridization analyses with the virion RNAs from the ST9 BWYV isolate.…”

Section: Analysis Of Cdna Clones and Their Hybridization Reactions Tomentioning

confidence: 99%

“…Unlike most luteoviruses, BWYV has a very wide host range, including many dicotyledonous and at least one monocotyledonous plant species (Duffus, 1960;Rochow & Duffus, 1981 ;Casper, 1988). The virions of BWYV and other luteoviruses are believed to be composed of a single species of ssRNA and a single capsid protein with an approximate Mr of 24000 (Falk & Duffus, 1984;Waterhouse et at., 1986). However, several luteoviruses have additional RNAs in the purified virions.…”

Section: Introductionmentioning

confidence: 99%

“…Some are heterologously encapsidated RNAs of related or unrelated viruses which are able to initiate infections independently from the associated luteovirus (Rochow, 1970;Falk et al, 1979;Waterhouse & Murant, 1983 ;Reddy et al, 1985). In at least two cases, however, the additionally encapsidated RNAs do not appear to be able to initiate independent infections (Takanami & Kubo, 1979;Falk & Duffus, 1984). Analysis of dsRNAs in extracts of luteovirus-infected plants have revealed not only genome-sized replicative form dsRNAs but also smaller dsRNAs (Gildow et al, 1983;Falk & Duffus, 1984), and recent hybridization and nucleotide sequence analysis of the genomic RNA for barley yellow dwarf luteovirus (BYDV) suggests that at least two subgenomic RNAs may be produced in BYDV-infected plants (Gerlach et al, 1987;Miller et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

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Complementary DNA Cloning and Hybridization Analysis of Beet Western Yellows Luteovirus RNAs

Falk

Chin

Duffus³

1989

Journal of General Virology

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SUMMARYComplementary DNAs to the virion RNAs of the ST9 strain of beet western yellows luteovirus (BWYV) were cloned and used for hybridization analyses. These showed that the two major virion ssRNAs, the genomic RNA, approximately 6 kb, and the 3.1 kb ST9-associated RNA, do not show detectable sequence homology. Evidence was also obtained concerning an ssRNA molecule of approximate size 0.72 kb, which hybridized with selected cDNA clones to the BWYV 6 kb genomic RNA; small amounts of it are associated with virions of ST9 and ST9-M BWYV isolates. Extracts of plants infected by ST9 and other BWYV isolates contained ssRNAs of approximately 6 kb, 2.9 kb and 0.72 kb in size. Extracts of ST9-infected plants also contained RNAs of approximately 3.1 kb and 0-48 kb which hybridized with selected cDNA clones prepared from the ST9-associated 3.1 kb virion RNA. cDNA clones of the ST9 virion 6 kb RNA also hybridized with the genomic 6 kb RNA of other BWYV isolates. None of the clones hybridized with preparations of other luteoviruses tested. No RNA molecule with a sequence related to the 3.1 kb ST9-associated virion RNA was detected in virions or plant tissues infected by other isolates of BWYV or other luteoviruses.

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Section: Discussionsupporting

confidence: 87%

Section: Analysis Of Cdna Clones and Their Hybridization Reactions Tomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Complementary DNA Cloning and Hybridization Analysis of Beet Western Yellows Luteovirus RNAs

Falk

Chin

Duffus³

1989

Journal of General Virology

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“…dsRNA samples were analysed by electrophoresis on 10 g L ¹1 agarose gels (Sambrook et al, 1989) as well as by electrophoresis on mini slab polyacrylamide gels (Smith et al, 1991). The doublestranded nature of the RNA extracted was confirmed by digestion of the presumed dsRNA with RNase A (Boehringer Mannheim) in high and low salt concentration (Falk & Duffus, 1984). Preliminary sizing of the ds RNA bands was carried out on 10 g L ¹1 agarose gels using dsDNA molecular weight markers (Bharathan et al, 1990).…”

Section: Double-stranded Rna Analysismentioning

confidence: 99%

Preliminary investigation of the causal agent(s) of a disease causing leaf curl of tobacco in South Africa

et al. 1997

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A high incidence (86%) of potyvirus infection was noted in tobacco plants exhibiting a form of leaf curl in South Africa. Despite leaf curl being reported in the literature to be of geminiviral aetiology, no geminiviruses were detected. Furthermore, no other virus particles were detected by virus purification, TEM and serology. Twelve species of dsRNA were consistently isolated from these tobacco plants, but were absent from other forms of leaf curl-affected and healthy tobacco. Aphid and mechanical inoculation demonstrated that the purified potyvirus(es) did not cause leaf curl symptoms, but rather mild mottle and mosaic symptoms in tobacco. Partial characterization of the potyvirus preparation showed a possible relationship to a South African strain of potato virus Y. Because potyvirus-inoculated plants did not manifest leaf curl symptoms, and because leaf curl symptoms were noted in some plants not infected with a potyvirus, it was concluded that the potyvirus is not involved in the leaf curl aetiology, but causes a latent infection, the symptoms of which are masked. The pattern of the dsRNA banding, induction of enations and lack of mechanical and seed transmission are common to plant reoviruses. The possibility of a phytoreovirus involvement in this form of leaf curl is currently being investigated. The results from this study suggest that tobacco leaf curl disease worldwide, with regard to geminiviruses, be re-evaluated.

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Signal interactions in pathogen and insect attack: expression of lipoxygenase, proteinase inhibitor II, and pathogenesis-related protein P4 in the tomato,Lycopersicon esculentum

Fidantsef

Stout

Thaler

et al. 1999

Physiological and Molecular Plant Pathology

192

125

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Identification of Small Single- and Double-Stranded RNAs Associated with Severe Symptoms in Beet Western Yellows Virus-InfectedCapsella bursa-pastoris

Cited by 34 publications

References 0 publications

Complementary DNA Cloning and Hybridization Analysis of Beet Western Yellows Luteovirus RNAs

Complementary DNA Cloning and Hybridization Analysis of Beet Western Yellows Luteovirus RNAs

Preliminary investigation of the causal agent(s) of a disease causing leaf curl of tobacco in South Africa

Signal interactions in pathogen and insect attack: expression of lipoxygenase, proteinase inhibitor II, and pathogenesis-related protein P4 in the tomato,Lycopersicon esculentum

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