Identification of Snp´s in the <i>Ace-1</i> Gene of <i>Spodoptera frugiperda</i>Associated with Resistance to Organophosphorus Insecticides

Herrera-Mayorga, Verónica; Bello-Ruiz, Dalila G.; Paredes-Sánchez, Francisco Alejandro; Segovia-Tagle, Veronica; García-Aguirre, Karol Karla; Lara-Ramírez, Edgar E.; Rivera, Gildardo

doi:10.3958/059.043.0404

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…Biochemical and molecular studies have shown that insensitivity of the target sites is an important resistance mechanism to both OPs and pyrethroids in S. frugiperda , although a metabolic mechanism is also involved (Yu et al ., 2003; Carvalho et al ., 2013). Three amino acid substitutions (A201S, G227A and F290V, numbered as Torpedo californica acetylcholinesterase, PDB ID: 1EA5) in the OP target protein (acetylcholinesterase‐1, AChE‐1), and three point mutations (T929I, L932F and L1014F, numbered as Musca domestica sodium channel, GenBank X96668) in the pyrethroid target protein (voltage‐gated sodium channel, VGSC) have been identified to be associated with resistance to OPs and pyrethroids respectively in Brazilian and Mexican field‐derived populations of S. frugiperda (Carvalho et al ., 2013; Herrera‐Mayorga et al ., 2018). More recently, the I4734M mutation in S. frugiperda ryanodine receptor (SfRyR) (equivalent to I4790M in Plutella xylostella RyR) was associated with 225‐fold and >5 400‐fold resistance to chlorantraniliprole and flubendiamide respectively in a laboratory‐selected population of FAW from Brazil (Boaventura et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

Whole‐genome sequencing to detect mutations associated with resistance to insecticides and Bt proteins in Spodoptera frugiperda

et al. 2020

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The fall armyworm (FAW), Spodoptera frugiperda, is a major pest native to the Americas that has recently invaded the Old World. Point mutations in the target-site proteins acetylcholinesterase-1 (ace-1), voltage-gated sodium channel (VGSC) and ryanodine receptor (RyR) have been identified in S. frugiperda as major resistance mechanisms to organophosphate, pyrethroid and diamide insecticides respectively. Mutations in the adenosine triphosphate-binding cassette transporter C2 gene (ABCC2) have also been identified to confer resistance to Cry1F protein. In this study, we applied a whole-genome sequencing (WGS) approach to identify point mutations in the target-site genes in 150 FAW individuals collected from China, Malawi, Uganda and Brazil. This approach revealed three amino acid substitutions (A201S, G227A and F290V) of S. frugiperda ace-1, which are known to be associated with organophosphate resistance. The Brazilian population had all three ace-1 point mutations and the 227A allele (mean frequency = 0.54) was the most common. Populations from China, Malawi and Uganda harbored two of the three ace-1 point mutations (A201S and F290V) with the 290V allele (0.47-0.58) as the dominant allele. Point mutations in VGSC (T929I, L932F and L1014F) and RyR (I4790M and G4946E) were not detected in any of the 150 individuals. A novel 12-bp insertion mutation in exon 15 of the ABCC2 gene was identified in some of the Brazilian individuals but absent in the invasive populations. Our results not only demonstrate robustness of the WGS-based genomic approach for detection of resistance mutations, but also provide insights for improvement of resistance management tactics in S. frugiperda.

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Section: Introductionmentioning

confidence: 99%

Whole‐genome sequencing to detect mutations associated with resistance to insecticides and Bt proteins in Spodoptera frugiperda

et al. 2020

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“…Based on previous studies, the A201S, G227A, and F290V mutations of acetylcholinesterase 1 (AChE1) within FAW will lead to resistance to organophosphate and carbamate insecticides [ 11 , 12 , 53 ]. A recent study in Sichuan Province’s Miyi region conducted biological assays and found high chlorpyrifos resistance in FAW [ 36 ], consistent with Wu et al’s (2015) finding that certain mutations in AChE may confer chlorpyrifos resistance in Apolygus lucorum [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…A recent study in Sichuan Province’s Miyi region conducted biological assays and found high chlorpyrifos resistance in FAW [ 36 ], consistent with Wu et al’s (2015) finding that certain mutations in AChE may confer chlorpyrifos resistance in Apolygus lucorum [ 54 ]. Replacement of the amino acid phenylalanine with valine was identified as reducing the available space within the acyl binding site for organophosphates, reducing binding [ 53 ]. The studies collectively indicate that mutations impacting the active binding site can diminish insecticide sensitivity.…”

Section: Discussionmentioning

confidence: 99%

Discovery of Novel Potential Insecticide-Resistance Mutations in Spodoptera frugiperda

Cai,

Chen,

et al. 2024

Insects

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The fall armyworm (FAW), Spodoptera frugiperda, is a worldwide agricultural pest that invaded China in 2018, and has developed resistance to multiple insecticides. The evolution of insecticide resistance is facilitated by mutations of target genes responsible for conferring resistance. In this study, amplicon sequencing analyzed 21 sites in six resistance genes. In addition to known mutations, unknown variants were also found, including novel variants: F290C (ace-1 gene, 0.1% frequency), I1040T/V (CHSA gene, 0.1% frequency), A309T (GluCl gene, 0.1% frequency), and I4790T/V (RyR gene, 0.1% frequency). Additionally, molecular docking was employed to investigate the impact of the aforementioned new mutations on insecticide binding to proteins. The analyses indicated that the binding abilities were reduced, similar to the resistance mutations that were reported, implying these novel mutations may confer transitional resistance. This study may provide a foundation for understanding the functions of these novel mutations in the evolutionary processes that drive the emergence of insecticide resistance in this invasive species.

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“…Research in recent years has shown that the resistance to diamides (Bolzan et al 2019), spinosad (Lira et al 2020), and benzoylurea (Nascimento et al 2016) was also developed. The resistance mechanism of S. frugiperda to insecticides mainly involves target and metabolic resistance (Herrera-Mayorga et al 2018, Liu et al 2019). Target mutation is one of the causes of resistance, such as a ryanodine receptor target-site of I4790M mutation leading to resistance to diamide insecticide (Boaventura et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Chlorpyrifos and chlorfenapyr resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) relies on UDP-glucuronosyltransferases

Zhang

2023

Journal of Economic Entomology

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Fall armyworm, Spodoptera frugiperda (J. E. Smith), has become an important agricultural pest worldwide. S. frugiperda is mainly controlled by the chemical insecticides, whereas the frequent application of insecticides would result in the resistance development. Insect uridine diphosphate-glucuronosyltransferases (UGTs), as phase II metabolism enzymes, play vital roles in the breakdown of endobiotic and xenobiotics. In this study, 42 UGT genes were identified by RNA-seq, including 29 UGT genes were elevated compared to the susceptible population, and the transcript levels of 3 UGTs (UGT40F20, UGT40R18, and UGT40D17) were increased by more than 2.0-fold in the field populations. Expression pattern analysis revealed that S. frugiperda UGT40F20, UGT40R18, and UGT40D17 were increased by 6.34-, 4.26-, and 8.28-fold, compared the susceptible populations, respectively. The expression of UGT40D17, UGT40F20, and UGT40R18 was affected after exposure to phenobarbital, chlorpyrifos, chlorfenapyr, sulfinpyrazone, and 5-nitrouracil. The induced expression of UGT genes may have improved UGT enzymatic activity, while the inhibition of UGTs genes expression may decreased UGT enzymatic activity. Sulfinpyrazone, and 5-nitrouracil, significantly increased the toxicity of chlorpyrifos and chlorfenapyr, as well as phenobarbital significantly reduced the toxicity of chlorpyrifos and chlorfenapyr against the susceptible populations and field populations of S. frugiperda. The suppression of UGTs (UGT40D17, UGT40F20, and UGT40R18) significantly increased the insensitivity of the field populations to chlorpyrifos and chlorfenapyr. These findings strongly supported our viewpoint that UGTs may play a critical role in insecticide detoxification. This study provides a scientific basis for the management of S. frugiperda.

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Identification of Snp´s in the Ace-1 Gene of Spodoptera frugiperdaAssociated with Resistance to Organophosphorus Insecticides

Cited by 9 publications

References 15 publications

Whole‐genome sequencing to detect mutations associated with resistance to insecticides and Bt proteins in Spodoptera frugiperda

Whole‐genome sequencing to detect mutations associated with resistance to insecticides and Bt proteins in Spodoptera frugiperda

Discovery of Novel Potential Insecticide-Resistance Mutations in Spodoptera frugiperda

Chlorpyrifos and chlorfenapyr resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) relies on UDP-glucuronosyltransferases

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