Identification of SNPs within the PRLR gene and effects on maternal behavior in sheep

Prolactin is an anterior pituitary peptide hormone involved in many different endocrine activities and is essential for reproductive performance. This action is mediated by its receptor, the prolactin receptor, encoded by the PRLR gene. In this study, we sequenced and characterized the Mediterranean river buffalo PRLR gene (from exon 3 to 10), and we found remarkable genetic diversity. In particular, we found 24 intronic polymorphisms and 13 exonic SNPs, seven of which were non-synonymous. Furthermore, the polymorphisms identified in the 3'-UTR were investigated to establish their possible influence on microRNA binding sites. Considering all the amino acid changes and the observed allelic combinations, it is possible to deduce at least six different translations of the buffalo prolactin receptor and, consequently, the presence at the PRLR gene of at least six alleles. Furthermore, we identified a deletion of a CACTACC heptamer between nucleotides 1102 and 1103 of exon 10 (3'-UTR), and we developed an allele-specific PCR to identify the carriers of this genetic marker. Finally, the SNP g.11188A>G, detected in exon 10 and responsible for the amino acid replacement p.His328Arg, was genotyped in 308 Italian Mediterranean river buffaloes, and an association study with milk fat traits was carried out. The statistical analysis showed a tendency that approached significance for the AA genotype with higher contents of odd branched-chain fatty acids. Thus, our results suggest that the PRLR gene is a good candidate for gene association studies with qualitative traits related to buffalo milk production.

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“…), the induction of maternal behavior in sheep (Wang et al . ) and litter size in goat (Hou et al . ).…”

Section: Introductionmentioning

confidence: 99%

Remarkable genetic diversity detected at river buffalo prolactin receptor (PRLR) gene and association studies with milk fatty acid composition

et al. 2018

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“…241 In ewes, an exon 10 PRLR SNP was associated both with larger litter size 242 and maternal behavior. 243 In Wijian white geese, multiple SNPs in exon 10 of the PRLR gene were identified, with one haplotype being associated with more eggs produced. 244 Similarly, haplotypes reflecting 6 SNP in the chicken PRLR (5 intronic and 1 in the final exon) were associated with egg production traits including the total number of eggs laid at 300 days of age, and age at first lay.…”

Section: Reproductive Traitsmentioning

confidence: 99%

“…The most notable of the mutations, g.184822G/A (p.Ser485Asn), can be associated with increased litter weight and birth weight, but not litter size 241 . In ewes, an exon 10 PRLR SNP was associated both with larger litter size 242 and maternal behavior 243 . In Wijian white geese, multiple SNPs in exon 10 of the PRLR gene were identified, with one haplotype being associated with more eggs produced 244 .…”

Section: Prlr Genetic Variants In Quantitative Association Studiesmentioning

confidence: 99%

The prolactin receptor: A cross‐species comparison of gene structure, transcriptional regulation, tissue‐specificity, and genetic variation

Banks,

Trott,

Hovey

2024

J Neuroendocrinology

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The conserved and multifaceted functions of prolactin (PRL) are coordinated through varied distribution and expression of its cell‐surface receptor (PRLR) across a range of tissues and physiological states. The resultant heterogeneous expression of PRLR mRNA and protein across different organs and cell types supports a wide range of PRL‐regulated processes including reproduction, lactation, development, and homeostasis. Genetic variation within the PRLR gene also accounts for several phenotypes impacting agricultural production and human pathology. The goal of this review is to highlight the many elements that control differential expression of the PRLR across tissues, and the various phenotypes that exist across species due to variation in the PRLR gene.

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“…Saanen, Guanzhong, and Boer goat breeds (Hou et al, 2014), Chinese Hu sheep (Wang et al, 2015), Chinese Haimen goat (Dejun Ji et al, 2016), Gaddi goat (Sankhyan et al, 2019), Shaanbei white cashmere goat (Xin Feng Liu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genetic Polymorphism of Insulin-Like Growth Factor 1 and Prolactin Receptor Genes in Surti and Mehsana Goats by PCR-RFLP

Ansari

Brahmkshtri

Ramani

et al. 2022

ijvsbt

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The present study was planned with the objective to amplify 5’NCR Insulin-like growth factor 1 (IGF1) and 3’UTR Prolactin receptor (PRLR) genes using caprine specific primers by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Surti and Mehsana goats. IGF1 gene 5’NCR was found to be monomorphic on restriction digestion with HaeIII, which revealed only one genotype GG in both Surti and Mehsana goats. The allele frequency of G was found to be 1.00 in both the breeds. Restriction digestion of PRLR gene 443 bp fragment with AluI also showed monomorphic pattern. Only one genotype CC was found with an allele frequency of 1.0 in both the Surti and Mehsana goats.

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Identification of SNPs within the PRLR gene and effects on maternal behavior in sheep

Cited by 7 publications

References 24 publications

Remarkable genetic diversity detected at river buffalo prolactin receptor (PRLR) gene and association studies with milk fatty acid composition

Remarkable genetic diversity detected at river buffalo prolactin receptor (PRLR) gene and association studies with milk fatty acid composition

The prolactin receptor: A cross‐species comparison of gene structure, transcriptional regulation, tissue‐specificity, and genetic variation

Genetic Polymorphism of Insulin-Like Growth Factor 1 and Prolactin Receptor Genes in Surti and Mehsana Goats by PCR-RFLP

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