Identification of SNPs within the sheep PROP1 gene and their effects on wool traits

XianCun, Zeng; Chen, Hanying; Jia, Bin; Zhao, Zhangwu; Hui, Wenqiao; ZunBao, Wang; Du, Yi

doi:10.1007/s11033-010-0416-4

Cited by 20 publications

(10 citation statements)

References 24 publications

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“…In this study, we detected one SNP located in the 20.9 Mb regions on OAR25. Moreover there were one QTLs detected on OAR5 and OAR6, respectively [5] , [48] . However, in this study, there were no significant SNP markers identified on these chromosomes.…”

Section: Resultsmentioning

confidence: 97%

“…The limitations of QTL mapping using linkage analysis (LA) and/or linkage disequilibrium (LD), based on panels of low to moderate-density markers, have been well documented [2] , [3] . Only a few major genes, e.g., the KRTAP6 gene (keratin-associated protein 6) [4] , the PROP1 gene (PROP paired-like homeobox 1) [5] and ADRB3 (beta3-adrenergic receptor) [6] , have been associated with wool traits using QTL linkage analyses or candidate gene studies.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Association Study for Wool Production Traits in a Chinese Merino Sheep Population

et al. 2014

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Genome-wide association studies (GWAS) provide a powerful approach for identifying quantitative trait loci without prior knowledge of location or function. To identify loci associated with wool production traits, we performed a genome-wide association study on a total of 765 Chinese Merino sheep (JunKen type) genotyped with 50 K single nucleotide polymorphisms (SNPs). In the present study, five wool production traits were examined: fiber diameter, fiber diameter coefficient of variation, fineness dispersion, staple length and crimp. We detected 28 genome-wide significant SNPs for fiber diameter, fiber diameter coefficient of variation, fineness dispersion, and crimp trait in the Chinese Merino sheep. About 43% of the significant SNP markers were located within known or predicted genes, including YWHAZ, KRTCAP3, TSPEAR, PIK3R4, KIF16B, PTPN3, GPRC5A, DDX47, TCF9, TPTE2, EPHA5 and NBEA genes. Our results not only confirm the results of previous reports, but also provide a suite of novel SNP markers and candidate genes associated with wool traits. Our findings will be useful for exploring the genetic control of wool traits in sheep.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study for Wool Production Traits in a Chinese Merino Sheep Population

et al. 2014

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“…35 Polymorphisms in this gene have been associated with production traits in livestock animals, such as cattle, 40 goats 41,42 and sheep. 43 The marker f68558 is probably not the causal locus that is responsible for growth trait variation in C. farreri . Instead, we think it is more likely that f68558 is in tight linkage disequilibrium with the causal mutation that is possibly located in the regulatory region (e.g.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution Linkage and Quantitative Trait Locus Mapping Aided by Genome Survey Sequencing: Building Up An Integrative Genomic Framework for a Bivalve Mollusc

et al. 2013

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Genetic linkage maps are indispensable tools in genetic and genomic studies. Recent development of genotyping-by-sequencing (GBS) methods holds great promise for constructing high-resolution linkage maps in organisms lacking extensive genomic resources. In the present study, linkage mapping was conducted for a bivalve mollusc (Chlamys farreri) using a newly developed GBS method—2b-restriction site-associated DNA (2b-RAD). Genome survey sequencing was performed to generate a preliminary reference genome that was utilized to facilitate linkage and quantitative trait locus (QTL) mapping in C. farreri. A high-resolution linkage map was constructed with a marker density (3806) that has, to our knowledge, never been achieved in any other molluscs. The linkage map covered nearly the whole genome (99.5%) with a resolution of 0.41 cM. QTL mapping and association analysis congruously revealed two growth-related QTLs and one potential sex-determination region. An important candidate QTL gene named PROP1, which functions in the regulation of growth hormone production in vertebrates, was identified from the growth-related QTL region detected on the linkage group LG3. We demonstrate that this linkage map can serve as an important platform for improving genome assembly and unifying multiple genomic resources. Our study, therefore, exemplifies how to build up an integrative genomic framework in a non-model organism.

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“…There were three mutation sites of P2 site in RXRG that were G, T and A by comparing with the reported ovis aries RXRG sequences, respectively, among which AAgenotype with an A → G mutation in 131bp and BB-genotype with a G → A mutation in 32bp were not cause amino acid changes. However, these mutations were not correlated with protein expression and therefore these mutations had not influenced gene translation [19] . Association analysis between genotype effect and twinning trait in China Merino sheep found that P2 mutation rate was higher, which showed that there were significant differences in the genotype distribution of among singleton and twins groups (P<0.05) and the different genotypes have a greater impact on the phenomenon of sheep twins that suggested that RXRG could affect the twinning trait in China Merino sheep.…”

Section: Discussionmentioning

confidence: 94%

Koyun RXRG Geninde SNP İdentifikasyonu ve İkizlik İle İlişkisi

Zhao¹,

Heng²,

Yu³

et al. 2017

Kafkas Univ Vet Fak Derg

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RXRG (Retinoic X receptor-gamma) gene was originally associated with fetal development and reproduction in human beings and animals, which was used to detect genetic variation that was associated with growth, reproduction, metabolism trait selection and breeding. The aim of this study was to detect RXRG gene mutation of the exon 2 and its association with twinning trait in 313 sheep and calculate litter size, genotype frequency in Chinese Merino, Hu and Kazak sheep. Polymorphism of exon 1, exon 2, exon 10 of RXRG gene in the study were analyzed by PCR-SSCP, which showed that three genotypes of P2 fragment were significantly associated with twinning traits in the analyzed population (P=0.031). Analysis of four groups of sheep showed that there was a predominate gene (B-allele) that have higher twinning rates in these individuals, which indicated that the genotypes can be chosen for twinning according to predominate genotype. In conclusion, our results strongly suggest that polymorphisms of the RXRG gene could be a new choice for sheep breeding and genetics through marker-assisted selection (MAS). Keywords: Sheep, Retinoic X receptor-gamma gene, Twinning trait, PCR-SSCP Koyun RXRG Geninde SNP İdentifikasyonu ve İkizlik İle İlişkisiÖzet RXRG (Retinoik X reseptör-gama) geni ilk olarak hayvan ve insanlarda fetal gelişim ve üreme ile ilişkilendirildi ve büyüme, üreme, metabolizma özellik seleksiyonu ve üreme ile ilgili genetik varyasyonu belirlemek için kullanıldı. Bu çalışmanın amacı, ekzon 2 RXRG gen mutasyonunu ve 313 koyunda ikizlik ile ilişkisini belirleyerek Çin Merinos, Hu ve Kazak koyunlarında yavru sayısı ve genotip frekansını hesaplamaktır. Bu çalışmada RXRG geni exon 1, exon 2 ve exon 10 polimorfizmleri PCR-SSCP ile analiz edildi ve incelenen popülasyon içerisinde P2 parçacığının üç genotipinin anlamlı derecede ikizlik ile ilişkili olduğu belirlendi (P=0.031). Dört grup koyunun analizi sonucunda bir baskın genin (B-allel) bu bireylerde yüksek ikizlik oranına sahip olduğunu ve bu genotipin ikizlik için seçilebileceğini gösterdi. Sonuç olarak, elde ettiğimiz sonuçlar RXRG gen polimorfizminin Marker ilişkili seleksiyon (MAS) yoluyla koyun üretiminde ve genetiğinde kullanılabileceğini önermektedir.

show abstract

Identification of SNPs within the sheep PROP1 gene and their effects on wool traits

Cited by 20 publications

References 24 publications

Genome-Wide Association Study for Wool Production Traits in a Chinese Merino Sheep Population

Genome-Wide Association Study for Wool Production Traits in a Chinese Merino Sheep Population

High-Resolution Linkage and Quantitative Trait Locus Mapping Aided by Genome Survey Sequencing: Building Up An Integrative Genomic Framework for a Bivalve Mollusc

Koyun RXRG Geninde SNP İdentifikasyonu ve İkizlik İle İlişkisi

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