Identification of Somatomammotroph Cells in Lactating Goats &lt;i&gt;(Capra hircus)&lt;/i&gt; by Fluorescence and Immunogold Techniques

Sanchez, J.; Navarro, J.A.; Bernab&eacute;, A.; G&oacute;mez, S.; Garc&eacute;s, B.; G&oacute;mez, M.A.

doi:10.1159/000147553

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…Such PRL-and GH-containing bihormonal cells have been identified in the human (12), bovine (13), goat (14), sheep (15), bat (16), rat (17), mouse (18), and musk shrew (19) anterior pituitary gland. To our knowledge, this is the first report of the existence of mammosomatotrophs within the avian pituitary gland.…”

Section: Discussionmentioning

confidence: 99%

“…In mammalian mammosomatotrophs, GH and PRL were reported to occur both inside the same secretory granules (12) and in separate granules (22). In previous immunohistochemical studies, the occurrence of mammosomatotrophs was reported to be rare in the lactating goat (14), whereas in sheep, mammosomatotrophs comprised 0.15% of all pituitary cells (15). Mammosomatotrophs formed 5.6 -9.6% of all pituitary cells obtained from virgin, late lactating, and postweaning rats (2) in reverse hemolytic plaque assays, and their relative proportions did not significantly change at the above physiological states.…”

Section: Turkey Mammosomatotrophsmentioning

confidence: 98%

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Identification of Mammosomatotrophs in the Turkey Hen Pituitary: Increased Abundance during Hyperprolactinemia*

et al. 1998

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We have previously reported that the hyperprolactinemia in incubating turkey hens is associated with recruitment of lactotrophs in the pituitary gland. In this study we have used double immunofluorescence and in situ hybridization histochemistry to 1) identify mammosomatotrophs in the anterior pituitary gland of egg-laying turkey hens and incubating hens, and 2) verify PRL gene expression within mammosomatotrophs by colocalizing PRL messenger RNA in GHimmunoreactive (ir) cells. The pituitaries of laying and incubating turkey hens were collected, and the midsagittal sections were dual labeled for either PRL and GH or PRL messenger RNA and GH. The plasma PRL concentrations were higher in incubating hens (231 Ϯ 10.6 ng/ml) than in laying hens (43 Ϯ 7.4 ng/ml; P Ͻ 0.01). In the midsagittal pituitary sections, mammosomatotrophs were predominantly found scattered in the caudal lobe of the anterior pituitary gland, in the ventral half of the cephalic lobe, and at the junction of cephalic and caudal lobes. In incubating hens, the proportion of mammosomatotrophs was 7.4 Ϯ 1.52% (mean Ϯ SEM) of the total number of GH-ir and/or PRL-ir cells counted, which was significantly higher (P Ͻ 0.05) than that found in laying hens (0.6 Ϯ 0.23%). Furthermore, PRL gene expression was observed in many GH-ir cells in the incubating hen pituitary gland. These data suggest that 1) mammosomatotrophs are present in the turkey pituitary gland, and 2) there is an increased abundance of mammosomatotrophs in the incubating turkey hen that may contribute to hyperprolactinemia. (Endocrinology 139: [781][782][783][784][785][786] 1998)

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Section: Discussionmentioning

confidence: 99%

Section: Turkey Mammosomatotrophsmentioning

confidence: 98%

Identification of Mammosomatotrophs in the Turkey Hen Pituitary: Increased Abundance during Hyperprolactinemia*

et al. 1998

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“…Studies of pituitary cells have shown that GH and PRL cells are also derived from a common progenitor cell (Borrelli et al, 1989). MS cells are adenohypophyseal endocrine cells, which contain both GH and PRL, and are widely distributed in mammals (Fumagalli and Zanini, 1985;Nikitovitch-Winer et al, 1987;Asa et al, 1988;Ishibashi and Shiino, 1989a, b;Porter et al, 1990;Sá nchez et al, 1994;Vidal et al, 1995;Gómez et al, 1999) and hen (Ramesh et al, 1998). In fish, MS cells have only been reported during pituitary development in Sparus aurata by light microscopic immunocytochemistry (Villaplana et al, 2000).…”

mentioning

confidence: 99%

Immunocytochemical and ultrastructural characterization of mammosomatotrope‐, growth hormone‐, and prolactin‐cells from the gilthead sea bream (sparus aurata l., teleostei): an ontogenic study

Villaplana

Ayala

Hernández

et al. 2003

Journal of Morphology

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Growth hormone (GH), prolactin (PRL), and mammosomatotrope (MS) cells of gilthead sea bream, Sparus aurata, a teleost fish, were studied in specimens from hatching to 15 months (adults) using conventional electron microscopy and an immunogold method using anti-tilapia GH sera and anti-chum salmon PRL serum. MS cells, immunoreactive to both anti-GH sera and anti-PRL sera, had been first identified in fish in a previous study in newly hatched larvae and in older larvae and juvenile specimens of Sparus aurata by light microscopic immunocytochemistry. In the present work, MS cells reacted positively to immunogold label only in older larvae and juveniles and their secretory granules immunoreacted with both GH and PRL antisera or with only one of them. MS cells were ultrastructurally similar to the PRL cells, with which they coincided in time. This is the first report on the ultrastructural characterization of MS cells in fish. In adults, the secretory granules of GH cells (immunoreactive to anti-GH serum) were mainly round, of variable size, and had a homogeneous, highly electron-dense content. Irregularly shaped secretory granules were also present. PRL cells (immunoreactive to anti-PRL serum) were usually observed in a follicular arrangement; they showed few, small, and mainly round secretory granules with a homogeneous and high or medium electron-dense content. Some oval or elongated secretory granules were also observed. GH and PRL cells that showed involutive features were also found. In newly hatched larvae, GH, PRL, and MS cells could not be distinguished either by their ultrastructure or by the immunogold labeling of the secretory granules. In 1-day-old larvae, presumptive GH and PRL cells were observed according to their position in the pituitary gland. In 2-day-old larvae, a few cells showed some of the ultrastructural features described for GH and PRL cells of adults. During development, the number, size, and shape of the secretory granules in both cell types clearly increased and the organelles developed gradually. Some GH cells were found undergoing mitosis.

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Diseases of the Endocrine System

Ruffin¹,

Christmann²,

Pugh³

2002

Sheep &Amp; Goat Medicine

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Identification of Somatomammotroph Cells in Lactating Goats <i>(Capra hircus)</i> by Fluorescence and Immunogold Techniques

Cited by 5 publications

References 7 publications

Identification of Mammosomatotrophs in the Turkey Hen Pituitary: Increased Abundance during Hyperprolactinemia*

Identification of Mammosomatotrophs in the Turkey Hen Pituitary: Increased Abundance during Hyperprolactinemia*

Immunocytochemical and ultrastructural characterization of mammosomatotrope‐, growth hormone‐, and prolactin‐cells from the gilthead sea bream (sparus aurata l., teleostei): an ontogenic study

Diseases of the Endocrine System

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