Identification of some bacterial cell components by FT-IR spectroscopy

Helm, Dieter; Naumann, Dieter

doi:10.1111/j.1574-6968.1995.tb07393.x

Cited by 174 publications

(109 citation statements)

References 24 publications

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“…A broad stretch of C-O-C, C-O at 1000-1200 cm −1 corresponds to the presence of carbohydrates [39], so in the fingerprint region (region below 1500 cm −1 where bands characterise the molecule as a whole), the strongest absorption band at 1067 cm −1 is attributed to that substance is polysaccharide [40]. Strong absorption at 1643 cm −1 which corresponds to amide I > C O str and C-N bending of protein and peptide amines, and a peak at 1378 cm −1 could be assigned to C O str of the COO − and C-O bond from COO − [41,42]. The FT-IR spectra of the polymer evidenced the presence of carboxyl groups, which may serve as binding sites for divalent cations [31].…”

Section: Sugar Analysis and Infrared (Ft-ir) Spectroscopymentioning

confidence: 96%

Physicochemical properties of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir

Wang

Ahmed

Feng

et al. 2008

International Journal of Biological Macromolecules

218

116

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Section: Sugar Analysis and Infrared (Ft-ir) Spectroscopymentioning

confidence: 96%

Physicochemical properties of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir

Wang

Ahmed

Feng

et al. 2008

International Journal of Biological Macromolecules

218

116

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“…Other absorption bands were at 3449.40, 2,920 cm -1 , representing the presence of O-H bonding and C-H bond of aliphatic compounds, respectively (Pal and Paul 2002). The most prominent marker (ester carbonyl) band for PHB was at 1,740 cm -1 by Helm and Naumann (1995) and 1724.03 cm -1 for pure PHB by Misra et al (2000). Band absorption between 1,724 and 1,740 cm -1 is typical of PHA (Shamala et al 2003).…”

Section: Microscopic Detection Of Phamentioning

confidence: 96%

Characterization of polyhydroxyalkanoate and the phaC gene of Paracoccus seriniphilus E71 strain isolated from a polluted marine microbial mat

López‐Cortés¹,

Rodríguez‐Fernández²,

Latisnere-Barragán³

et al. 2009

World J Microbiol Biotechnol

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Polyhydroxyalakanote (PHA) was produced by the marine bacteria Paracoccus seriniphilus Strain E71. Three methods were used for screening PHA in this strain:(1) microscopic analysis, (2) specifically designed primers for amplify fragments of phaC gene from Gram negative bacteria, and (3) measurements using spectroscopy, calorimetry, thermogravimetry, and rheology. The polyhydroxyalkanoic acid synthase gene (phaC) sequence had 77% identity with the phaC gene of P. denitrificans PD1222 strain. Additionally, the translated sequence showed an 86% similarity with the amino acid sequence of the phaC gene N-terminal portion of the P. denitrificans PD1222 strain. Our phaC sequence was closely related to two phaC sequences that correspond to P. denitrificans; therefore, this is the first report of a sequence of phaC that codifies a poly-(3-hydroxyalkanoate) synthase class I, specifically a polybeta-hydroxybutyrate polymerase from the marine bacteria Paracoccus seriniphilus. The polymer PHA of E71 melts at 167.86°C (T m ), which corresponded to the fusion of the crystalline polymer and thermally degrades at 296.52°C, indicating that the biopolymer has good thermal stability. Rheology showed that this polymer behaves as a nonNewtonian fluid. All these characteristics suggest that the E71 strain produces a PHA that corresponds to the crystalline thermoplastic polymer PHB type.

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“…The use of FT-IR relies on the ability to assign infrared bands in the mid IR region (4000-500 cm −1 ) to certain cell components (Helm and Naumann 1995;Naumann 2000). This technique requires minimal sample handling, and the intact cells can be examined without lysis and extraction of the cell components.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of sorbitol during the vacuum drying of Lactobacillus helveticus: an FT-IR study

et al. 2010

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Although the addition of sugars to drying media is a general practice for the viability improvement of lactic acid starter cultures during drying, the mechanism behind the protective effects is not clear. To gain insight into the protective mechanisms of sugars, Lactobacillus helveticus was dried by vacuum drying in the presence of sorbitol, whose protective effects were shown in our previous study (Santivarangkna et al. Lett Appl Microbiol 42:271-276, 2009). In this study, membrane phase transition temperatures (T m ) of fresh cells and cells dried with and without sorbitol were measured by Fourier transform infrared spectroscopy (FT-IR). Generally, an increase in membrane phase transition temperature (T m ) is believed to cause membrane damage during drying. Lactobacillus helveticus cells exhibited the transitions at the temperatures of 15.5 and 37.5°C. Drying of cells without sorbitol increased the transition temperatures to 16.5 and 41.5°C, while it was depressed by 6 and 10.5°C when dried with sorbitol. The interaction between membrane phospholipids and sorbitol was observed from hydrogen-bonding sensitive C = O and P = O stretching bands. The position of the P = O band was shifted to the lower frequency in cells dried with sorbitol, which reflects the hydrogen bonding interaction. We suggest that sorbitol protects cells during drying by depressing T m via the interaction with phosphate groups of membranes.

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Identification of some bacterial cell components by FT-IR spectroscopy

Cited by 174 publications

References 24 publications

Physicochemical properties of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir

Physicochemical properties of exopolysaccharide produced by Lactobacillus kefiranofaciens ZW3 isolated from Tibet kefir

Characterization of polyhydroxyalkanoate and the phaC gene of Paracoccus seriniphilus E71 strain isolated from a polluted marine microbial mat

Protective effects of sorbitol during the vacuum drying of Lactobacillus helveticus: an FT-IR study

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