Identification of sources of resistance to wheat stem rust using molecular markers

Кохметова, Алма; Atishova, M. N.

doi:10.1134/s2079059712060081

Cited by 16 publications

(14 citation statements)

References 20 publications

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“…PCR-based DNA markers were used to check rust resistance genes among the 20 wheat genotypes and 22 markers were amplified [16]. Kokhmetova and Atishova [17] studied the presence of Sr genes ( Sr2 , Sr22 , Sr24 , Sr36 , and Sr46 ), which are effective against Ug99 in 88 cultivars of spring soft wheat in Kazakhstan. Haile et al [18] screened out 30 Sr genes using SSR and STS markers in 58 tetraploid wheat accessions of Ethiopia.…”

Section: Introductionmentioning

confidence: 99%

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Cao

Wang

et al. 2016

PLoS ONE

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Stem rust is one of the most potentially harmful wheat diseases, but has been effectively controlled in China since 1970s. However, the interest in breeding wheat with durable resistance to stem rust has been renewed with the emergence of Ug99 (TTKSK) virulent to the widely used resistance gene Sr31, and by which the wheat stem rust was controlled for 40 years in wheat production area worldwide. Yunnan Province, located on the Southwest border of China, is one of the main wheat growing regions, playing a pivotal role in the wheat stem rust epidemic in China. This study investigated the levels of resistance in key wheat cultivars (lines) of Yunnan Province. In addition, the existence of Sr25, Sr26, Sr28, Sr31, Sr32, and Sr38 genes in 119 wheat cultivars was assessed using specific DNA markers. The results indicated that 77 (64.7%) tested wheat varieties showed different levels of resistance to all the tested races of Puccinia graminis f. sp. tritici. Using molecular markers, we identified the resistance gene Sr31 in 43 samples; Sr38 in 10 samples; Sr28 in 12 samples, and one sample which was resistant against Ug99 (avirulent to Sr32). No Sr25 or Sr26 (effective against Ug99) was identified in any cultivars tested. Furthermore, 5 out of 119 cultivars tested carried both Sr31 and Sr38 and eight contained both Sr31 and Sr28. The results enable the development of appropriate strategies to breed varieties resistant to stem rust.

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Section: Introductionmentioning

confidence: 99%

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Cao

Wang

et al. 2016

PLoS ONE

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“…GeneRuler 50 + bp (50-1000 bp) (Thermo Scientific, USA) and Evrogen 50 + bp (50-700 bp, Evrogen, Russia) were used as molecular weight markers. To identify the Sr2 stem rust resistance gene, we used the molecular SSR marker Xgwm533, which is closely linked to this gene for use by authors [31,[34][35][36]. The diagnostic Xgwm533 marker fragment was 120 bp.…”

Section: Dna Extraction and Studymentioning

confidence: 99%

Identification of Stem Rust Resistance Genes in the Winter Wheat Collection from Southern Russia

Алабушев

Vozhzhova

Kupreyshvili

et al. 2019

Plants

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The high yield potential of winter wheats cannot be realized due to disease pressure under field conditions. One of the most harmful of such diseases is stem rust, hence the constant search for sources of resistance and the development of new varieties resistant to stem rust is of great relevance. This study deals with the identification of stem rust resistance genes in a collection of winter wheats grown in Southern Russia. This genepool has not been studied yet. A total of 620 samples of winter soft wheat from various ecological and geographical zones were tested under field conditions. To identify the specific genes or alleles responsible for resistance, all samples were genotyped using PCR. As a result, the groups of resistant samples, carrying the Sr2, Sr31, Sr38 and Sr44 genes in various combinations, were identified. Most of the stem rust resistance was provided by the presence of the effective Sr44 gene. This information can be used in the future breeding work for stem rust resistance.

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“…PCR-based DNA markers were used to check rust resistance genes among the 20 wheat cultivars that are majorly grown in Bale zone and eight markers associated with seven stem rust resistance genes were screened. Similar work were also conducted to study the presence of Sr genes (Sr2, Sr22, Sr24, Sr36 ,and Sr46), in 88 cultivars of spring soft wheat in Kazakhstan [14]. Additionally, 58 tetraploid wheat accessions of Ethiopia were screened for 30 Sr genes using SSR and STS markers [13].…”

Section: Identification Of Stem Rust Resistance Genes In Wheat Cultivmentioning

confidence: 99%

Seedling Resistance to Stem Rust (<i>Puccinia graminis</i> f.sp.<i>tritici</i>) and Molecular Marker Analysis of Resistance Genes in Some Wheat Cultivars

Letta¹

2018

PLANT

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Stem rust caused by Puccinia graminis Pers.f.sp.tritici Eriks.and E.Henn.(Pgt) is one of the most destructive diseases of wheat which causing considerable yield losses in wheat growing areas worldwide. It has become a renewed threat to global wheat production after the emergence and spread of race TTKSK (also known as Ug99) and related races from Africa. Races of the pathogen in the "Ug99 lineages" are of international concern due to their virulence for widely used stem rust resistance genes and their spread throughout Africa. Disease resistant cultivars provide one of the best means for controlling stem rust. Bale zone, located on the Southeast part of Ethiopia, is one of the main wheat growing regions, playing a pivotal role in the wheat stem rust epidemic in Ethiopia. This study investigated levels of resistance in key wheat cultivars (lines) grown in Bale zone using seedling resistance evaluation and marker aided selection. Twenty wheat cultivars were evaluated for their response to stem rust infection at seedling stage under green house condition. Wheat cultivars were challenged with four stem rust races viz TTKSK, TRTTF, TTTTF and JRQCQ. A high level of phenotypic variation was observed in response to these races in the test entries, allowing for selection in these germplasm as a pre-breeding work. Out of the tested cultivars, three wheat cultivars exhibited low infection types (0-2) response to all the four races and hence selected as a source of resistance to stem rust. In addition, the existence of Sr2, Sr22, Sr24, Sr25, Sr26, Sr35 and Sr36 genes in wheat cultivars were assessed using specific DNA markers. Using molecular markers, resistance gene Sr2 was identified in 2 cultivars and Sr24 in five cultivars. However, no Sr25, Sr26, Sr35 and Sr36 were identified in any cultivars tested using DNA markers. The results of both seedling evaluation and marker based resistance gene identification will enable to breed wheat varieties with durable resistance to stem rust disease.

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Identification of sources of resistance to wheat stem rust using molecular markers

Cited by 16 publications

References 20 publications

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Identification of Stem Rust Resistance Genes in the Winter Wheat Collection from Southern Russia

Seedling Resistance to Stem Rust (<i>Puccinia graminis</i> f.sp.<i>tritici</i>) and Molecular Marker Analysis of Resistance Genes in Some Wheat Cultivars

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Identification of sources of resistance to wheat stem rust using molecular markers

Cited by 16 publications

References 20 publications

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Seedling Resistance to Stem Rust and Molecular Marker Analysis of Resistance Genes in Wheat Cultivars of Yunnan, China

Identification of Stem Rust Resistance Genes in the Winter Wheat Collection from Southern Russia

Seedling Resistance to Stem Rust (&lt;i&gt;Puccinia graminis&lt;/i&gt; f.sp.&lt;i&gt;tritici&lt;/i&gt;) and Molecular Marker Analysis of Resistance Genes in Some Wheat Cultivars

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Seedling Resistance to Stem Rust (<i>Puccinia graminis</i> f.sp.<i>tritici</i>) and Molecular Marker Analysis of Resistance Genes in Some Wheat Cultivars