Identification of species-specific molecular markers in different farm animals by PCR-RFLP analysis

Khan, Waqas Ahmad

doi:10.19045/bspab.2018.70041

Cited by 4 publications

(11 citation statements)

References 1 publication

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“…Because it is critical for food safety, consumer demands, and law. For these using PCR based method is very trustworthy for the quality of food generally and the meat products specifically (Al-Sanjary, 2009;Farag et al, 2015, Khan et al, 2018and Dilger et al, 2020. As a result of both restriction enzymes that were done all samples separately at the same time, we can notice that the sausage was displayed mislabeling and marked as fraudulent because was not parallel to the chicken meat (positive control) and chicken meat was not used during the process of sausages.…”

Section: Resultsmentioning

confidence: 99%

“…It is more probable that meat of two or more species is mixed for fraudulent purpose during meat processing such as grinders, cutters, knives, choppers. In order to detect traces of meat mixtures samples have to be kept for PCR-RFLP method due to is a very powerful method (Khan et al, 2018). The results were achieved in utilizing the HinfI, RsaI restriction enzymes absolutely recommended that these results were suitable to authenticity and demonstrate unreal meat source and proposed to utilize other types to detect the real sausages species.…”

Section: Resultsmentioning

confidence: 99%

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Identification and Differentiation of Poultry Meat and Products Using PCR-RFLP Technique

Taha¹,

Khdr²,

Kareem³

2021

Mesopotamia Journal of Agriculture

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The mitochondrial cyt b gene plays a serious role in investigating untruthful meat species. This study aimed to authenticate the species of poultry products (Escallop, Nugget, Steak, and Sausage) depending on cyt b gene by using universal cyt b primer. DNA was isolated, and then a band of 359 bp of a mitochondrial cytochrome b gene was produced during the PCR amplification. The PCR products were exposed to Hinf1 and Rsa I restriction enzymes. The restricted fragments produced by restriction fragment length polymorphism technique (RFLP), were run by agarose gel electrophoresis. Results showed that all products had a similar band except sausage product does not follow the rule and showed mislabeling product by the REs, Two bands were yielded by HinfI RE for all products (114 and 245) bp with the differentiated sausage among other products based on the fake product (63 and 296) bp, while digestion by Rsa I produced three bands for escallop, nugget, and steak, (63, 100, 196), but only two bands for sausage was generated (148 and 211). As result, the study offered that analyzing meat products to detect the origin species via a PCR-RFLP technique by using these restriction enzymes can give reliable results. In short sausage is considered as fraud products because the results showed different bands as compared with poultry meat.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Identification and Differentiation of Poultry Meat and Products Using PCR-RFLP Technique

Taha¹,

Khdr²,

Kareem³

2021

Mesopotamia Journal of Agriculture

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“…But they investigated nine different meat species, excluding goat and dog and amplicons length was 710-bp (Haider et al, 2012), which is not more ideal for processed, cooked and stored meat samples due to fragmentation of DNA (Musto, 2011). Khan et al (2018) amplified a very short fragment (359-bp) of cytochrome b gene for authentication of animal species. But they used more than one restriction endonucleases (Tas1, Hinf ) for restriction profiling, and only considered the farm animals excluding dog and donkey meats (Khan et al, 2018).…”

Section: Rflp Profilingmentioning

confidence: 99%

“…Khan et al (2018) amplified a very short fragment (359-bp) of cytochrome b gene for authentication of animal species. But they used more than one restriction endonucleases (Tas1, Hinf ) for restriction profiling, and only considered the farm animals excluding dog and donkey meats (Khan et al, 2018).…”

Section: Rflp Profilingmentioning

confidence: 99%

Identification of Meat Species by PCR-RFLP Method using Single Set of Degenerative Primers

Asghar¹,

Malik²,

Rashid³

et al. 2021

SJA

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Electrophoresis, chromatography and enzyme-linked immunoassay are common analytical techniques (Hsieh, 2000;Floren et al., 2015). Different muscle proteins are separated by electrophoresis, but the requirement of baseline data and uniformity of protein patterns make their results elusive (Ilhak and Arslan, 2007). Chromatographic techniques differentiate among origin of meat by their fatty acid and protein composition (Chou et al., 2007), enzyme-linked immunoassay by reactivity of specific antibodies with Abstract | Due to religious and social concerns, identification of meat and meat products is a matter of great interest for general public. DNA-based identification methods are ideal and reliable. DNA was extracted from muscle tissues of cow, buffalo, goat, donkey and a dog. A set of degenerative primers was designed for Polymerase Chain Reaction (PCR) of the cytochrome b gene fragment. All PCR was accomplished at the same reaction conditions. Amplified products had a length of 400 bp, which was our estimated length. After the sequencing of these amplicons, In silico restriction analysis was performed for each specie. Restriction endonuclease TfiI was selected for further analysis. Restriction fragment length polymorphism (RFLP) was performed at the same reaction conditions for all species. RFLP analysis produced species specific restriction profiles. Comparing these profiles, we have identified the species origin of meat unambiguously.

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“…Several sources of meat products in different species mixed together to make a new inexpensive product and unnoticeable by physical testing (Ong et al, 2007). Regrettably, in many countries (specifically development countries) meat adulteration has always been a concern for several reasons such as health, economic and religious concerns (Khan et al, 2018). Thus, detection of the origin species existing in meat products has become vital to keep people from illegal and undesirable corruption (Ghovvati et al, 2009, Ciupa et al, 2012.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Raw Meat for Some Animal Species Using RFLP-PCR Technique

Taha

2031

Mesopotamia Journal of Agriculture

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Mitochondrial cytochrome b gene plays a serious role in studying adulteration of meat species. This research study designed to distinguish the raw meat species of sheep, goat, cattle and donkey using RFLP-PCR technique of a universal cyt b gene 359bp. Ten indigenous samples were collected from each animal in different parts of the body. All the samples were processed for DNA isolation and amplified with a Polymerase chain reaction (PCR). The amplicons were cleavage with HinfI and RsaI restriction enzymes, digestion of PCR product resulted in production of specific characterization bands for each species then analyzed by agarose electrophoresis. HinfI RE created three fragments for sheep, goat and cattle, with some similarities in a few bands between them, while yielded two bands for donkey. RsaI RE produced two bands for all species with different length except sheep and goats have the same length. Thus, results recommend that the RFLP-PCR technique with HinfI and RsaI play an important role to detect the animal meat species, since it is a fast, simple and easily handle method for identification of animal species.

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Identification of species-specific molecular markers in different farm animals by PCR-RFLP analysis

Cited by 4 publications

References 1 publication

Identification and Differentiation of Poultry Meat and Products Using PCR-RFLP Technique

Identification and Differentiation of Poultry Meat and Products Using PCR-RFLP Technique

Identification of Meat Species by PCR-RFLP Method using Single Set of Degenerative Primers

Molecular Detection of Raw Meat for Some Animal Species Using RFLP-PCR Technique

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