Identification of Specific DNA Fragments in the Superior Mutant Plant of Rodent Tuber Accession Pekalongan (<i>Typhonium flagelliforme</i>) Based on Sequencing Analysis

Abstract

Cancer is a large group of diseases that occur in almost any organ or tissue of the body when abnormal cells get out of normal and invade adjacent cells. In women especially, breast cancer is among the most common types of cancer. These cancer cells will form a tumor that can be seen on an x-ray. Some traditional breast cancer treatments have been carried out, including using local herbal plants such as rodent tubers (Typhonium flagelliforme). Rodent tuber contains stigmasterol which has a high anticancer effect and was found in several mutant clones. The purpose of this study was to analyze DNA differences between mutant and wild-type rodent tuber plants based on specific RAPD markers and sequence differences in each sample. The RAPD OPB18 primer was successfully amplifies polymorphic DNA bands with sizes ranging from 700 to 1000 bp. As a result of the sequencing analysis, separate segments in wild-type and mutant plants had different sequences. In comparing the test sequence to the NCBI database, it was found that wild-type rodent tuber samples matched Gossypium raimondii (81.82%) and BLAC (bulk highest anticancer compound) rodent tuber mutant plants has similarity with Slanum pinnasectum about 87.36%. Based on this results, the similarity of rodent tuber sequences is possible to be developed in the specific sequences that encode a gene, especially genes encoding anticancer compounds.

Understanding of genes encoding bioactive compounds from potential medicinal plants in Indonesia as cancer cell inhibitors

“…Lectins are non-enzyme proteins that include a minimum of one non-catalyzing site and can bind to particular targets reversibly. Several potential medicinal plants in Indonesia contain lectins, such as Pisum fulvum and P. sativum (Family: Fabaceae), T. flagelliforme (Family: Araceae), and Viscum album (Family: Santalaceae) (Yousefvand et al 2022;Yassin et al 2019;Sianipar et al 2022a). Lectins can act as anticancer agents with a general mechanism through the apoptosis process in cancer cells, so that cancer cell growth is inhibited.…”

“…Lectin genes were successfully detected in the potential of Indonesian medicinal plants such as Morus spp. (Family: Moraceae) (Wulandari et al 2018), T. flagelliforme (Family: Araceae) (Sianipar et al 2022a), and G. max (Family: Fabaceae) (Wang et al 2021).…”