Identification of Specific Variations within the HE, S1, and ORF4 Genes of Bovine Coronaviruses Associated with Enteric and Respiratory Diseases in Dairy Cattle

Gélinas, Anne-Marie; Sasseville, A. Marie-Josée; Dea, S.

doi:10.1007/978-1-4615-1325-4_9

Cited by 10 publications

(9 citation statements)

References 15 publications

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“…Until now, it has been unclear whether nasal and rectal isolates of BCoVs are the same virus or whether BCoV undergoes significant genetic changes during adaptation to different tissues of the host. In general, biological, antigenic, and genetic differences were found between BCoV-R and BCoV-E (Gelinas et al, 2001a(Gelinas et al, , 2001bHasoksuz et al, 1999;Lin et al, 2002). However, other investigators did not find consistent differences in biologic properties between BCoV-R and BCoV-E strains (Reynolds et al, 1985;Zhang et al, 1994a).…”

Section: Changes After Tissue Culture Adaptationmentioning

confidence: 89%

“…However, it is likely that for BCoV tissue tropism, shifts are determined by multiple factors involving a combination of genetic mutations. Others observed a truncated 4.9 kDa nonstructural protein of 29 aa in BCoV-R strains instead of 43 aa for BCoV-E strains and suggested that this protein could be involved in determining respiratory tropism (Gelinas et al, 2001b;Vijgen et al, 2006). But this hypothesis also has not been experimentally proven.…”

Section: Changes After Tissue Culture Adaptationmentioning

confidence: 99%

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Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation

Zhang

Hasöksüz

Spiro³

et al. 2007

Virology

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The genetic diversity of 2 pairs (AH65 and AH187) of wild type bovine coronaviruses (BCoV) sequenced directly from nasal (respiratory) and rectal (enteric) swabs of two feedlot calves with respiratory and enteric symptoms [Hasoksuz, M., Sreevatsan, S., Cho, K.O., Hoet, A.E., Saif, L.J., 2002b. Molecular analysis of the S1 subunit of the spike glycoprotein of respiratory and enteric bovine coronavirus isolates. Virus Res. 84 (1-2), 101-109.]. was analyzed. Sequence analysis of the complete genomes revealed differences at 123 and 149 nucleotides (nt) throughout the entire genome between the respiratory and enteric strains for samples AH65 and AH187, respectively, indicating the presence of intra-host BCoV quasispecies. In addition, significant numbers of sequence ambiguities were found in the genomes of some BCoV-R and BCoV-E strains, suggesting intra-isolate quasispecies. The tissue culture (TC) passaged counterparts of AH65 respiratory BCoV (AH65-R-TC) and enteric BCoV (AH65-E-TC) were also sequenced after 14 and 15 passages and 1 plaque purification in human rectal tumor cells (HRT-18), respectively. Compared to the parental wild type strains, tissue culture passage generated 104 nt changes in the AH65-E-TC isolate but only 8 nt changes in the AH65-R-TC isolate. Particularly noteworthy, the majority of nucleotide changes in the AH65-E-TC isolate occurred at the identical positions as the mutations occurring in the AH65-R strain from the same animal. These data suggest that BCoV evolves through quasispecies development, and that enteric BCoV isolates are more prone to genetic changes and may mutate to resemble respiratory BCoV strains after tissue culture passage.

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Section: Changes After Tissue Culture Adaptationmentioning

confidence: 89%

Section: Changes After Tissue Culture Adaptationmentioning

confidence: 99%

Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation

Zhang

Hasöksüz

Spiro³

et al. 2007

Virology

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“…Diff erentiating between enteric and respiratory BCoV isolates is a problem, however, with contradictory conclusions within in the scientifi c literature. While several laboratories did not confi rm consistent diff erences between isolates originating from enteric and respiratory organs (Liu et al, 2006;Decaro et al, 2008;Lojkic et al, 2015), others have found signifi cant diff erences (Chouljenko et al, 2001;Gelinas et al, 2001;Hasoksuz et al, 2002;Park et al, 2006;Fulton et al, 2013). Most probably a comparative sequence analysis of the entire genome of a greater number of coronavirus isolates, including antigenic studies, will defi nitely resolve the issue.…”

Section: Figmentioning

confidence: 98%

Genetic variability of the S1 subunit of enteric and respiratory bovine coronavirus isolates

Vilček¹,

Jacková²,

Kolesárová³

et al. 2017

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Bovine coronavirus (BCoV) is considered an important pathogen in cattle worldwide. It is a causative agent of enteric and respiratory diseases of cattle. The S1 subunit of the viral S glycoprotein is responsible for virus binding to host-cell receptors, induction of neutralizing antibody and hemagglutinin activity. The entire S1 genomic region (2304 bp) of two enteric bovine coronavirus isolates from Austria, one respiratory and one enteric isolate from Slovakia were analyzed at the genetic level. The comparative analysis of those four isolates revealed 97.1-98.6% similarity at the nucleotide and 95.6-98.6% at the amino acid level. No differences between enteric and respiratory isolates were observed at the genetic level. The isolates were clustered in the phylogenetic tree with European isolates independently of their enteric or respiratory origin.

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“…Researchers have sequenced the partial or full-length S gene of multiple BCoV strains to ascertain the genetic basis of the broad host range of BCoV (see section on Epidemiology) and occurrence of the distinct clinical syndromes. Several groups have compared the S (or S1) or full-length genomic sequences 22,[26][27][28][32][33][34][35][36] of WD or respiratory and enteric BCoV isolates, including isolates from the same animal. The porcine respiratory CoV evolved as an S gene deletion mutant (deletions of 621-681nuceotides) of swine transmissible gastroenteritis virus, acquiring an almost exclusive respiratory tropism.…”

Section: Viral Characteristicsmentioning

confidence: 99%

“…37 No similar large S gene deletions were detected in respiratory BCoV strains, most of which also possess an enteric tropism as revealed by calf challenge studies. 29 Focusing on the hypervariable region (amino acids [aa] 452-593) containing the neutralizing epitope (S1B) of the S1 subunit, 4 groups 22,26,27,36 reported that respiratory strains (or respiratory and enteric isolates from the same feedlot calf) had changes in aa residues 510 and 531 compared with the reference enteric Mebus strain and a WD strain (DBA). One of the polymorphic positions (aa 531) discriminated between enteric (aspartic acid or asparagine) and respiratory (glycine) BCoV strains in 2 studies, 26,36 but not in others.…”

Section: Viral Characteristicsmentioning

confidence: 99%

Bovine Respiratory Coronavirus

Saif

2010

Veterinary Clinics of North America: Food Animal Practice

205

281

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Bovine coronaviruses (BCoVs) cause respiratory and enteric infections in cattle and wild ruminants. BCoV is a pneumoenteric virus that infects the upper and lower respiratory tract and intestine. It is shed in feces and nasal secretions and also infects the lung. BCoV is the cause of 3 distinct clinical syndromes in cattle: (1) calf diarrhea, (2) winter dysentery with hemorrhagic diarrhea in adults, and (3) respiratory infections in cattle of various ages including the bovine respiratory disease complex or shipping fever of feedlot cattle. No consistent antigenic or genetic markers have been identified to discriminate BCoVs from the different clinical syndromes. At present, there are no BCoV vaccines to prevent respiratory BCoV infections in cattle, and the correlates of immunity to respiratory BCoV infections are unknown. This article focuses on respiratory BCoV infections including viral characteristics; epidemiology and interspecies transmission; diagnosis, pathogenesis, and clinical signs; and immunity and vaccines.

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Identification of Specific Variations within the HE, S1, and ORF4 Genes of Bovine Coronaviruses Associated with Enteric and Respiratory Diseases in Dairy Cattle

Cited by 10 publications

References 15 publications

Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation

Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation

Genetic variability of the S1 subunit of enteric and respiratory bovine coronavirus isolates

Bovine Respiratory Coronavirus

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