Identification of specificities of antibodies against human leukocyte antigens in blood donors

Endres, Robert O.; Kleinman, Steven; Carrick, Danielle M.; Steele, Whitney R.; Wright, David J.; Norris, Philip J.; Triulzi, Darrell J.; Kakaiya, Ram; Busch, Michael P.

doi:10.1111/j.1537-2995.2010.02589.x

Cited by 44 publications

(52 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ten negative control sera were used to calculate the baseline normalized mean fluorescence intensity (MFI) and the signal-to-noise ratio for every individual bead as follows: Baseline normalized MFI = (MFI bead mother -MFI negative control bead [NCB]) -mean (MFI bead negative control 1-10 -MFI NCB negative control [1][2][3][4][5][6][7][8][9][10] ); Ratio = (MFI bead mother / MFI NCB) / 2 * (mean+3SD [MFI bead negative control 1-10 / MFI NCB negative…”

Section: Hla-antibody Analysis and Assignment As Child-specificmentioning

confidence: 99%

“…Pregnancy-induced sensitization has been reported in the range of 10-40% using CDC-methodology or solidphase assays (6)(7)(8)(9)(10). However, these studies had some limitations, which can under-or overestimate the frequency of sensitization.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, the use of less sensitive CDC-methodology or analysis in sera obtained many years after pregnancy might miss occurred sensitization (5)(6)(7)(8)(9). By contrast, describing the overall rate of HLA-antibody positivity after pregnancy without assessing the child-specificity might overestimate the frequency of pregnancy-induced sensitization (6,(8)(9)(10). It is well known that the rate of pregnancy-induced sensitization is related to the number of pregnancies, while other influencing factors are largely unknown and have not been investigated (6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Frequency and Determinants of Pregnancy-Induced Child-Specific Sensitization

Hönger

Fornaro

Granado

et al. 2013

American Journal of Transplantation

View full text Add to dashboard Cite

The aim of this study was to define the frequency and determinants of pregnancy-induced child-specific sensitization shortly after full-term delivery using sensitive single HLA-antigen beads (SAB) and high resolution HLA-typing of the mothers and their children (n = 301). A positive SAB result was defined by a background normalized ratio >1 or a mean fluorescence intensity (MFI) >300, >500 and >1000, respectively. The overall frequency of pregnancy-induced sensitization determined by SAB shortly after full-term delivery was between 45% (MFI > 1000 cut-off) and 76% (ratio cut-off). The rate of child-specific sensitization at the HLA-A/B/C/DRB1 loci was between 28% (MFI > 1000 cut-off) and 38% (ratio cut-off). The number of live birth was associated with a higher frequency of sensitization, which was driven by childspecific, but not third party HLA-antibodies. There was a clear hierarchy of sensitization among the investigated loci (B-locus: 31%; A-locus: 26%; DRB1-locus: 20%; C-locus: 15%; p < 0.0001). Some mismatched paternal HLA-antigens led to a significantly higher rate of sensitization than the average (e.g. HLA-A2, HLA-B49, HLA-B51, HLA-C * 15). Furthermore, the mother's own HLA-phenotype-especially HLA-A/B homozygositywas associated with a higher rate and broadness of sensitization. The number of mismatched HLA-A/B/C eplets strongly correlated with the rate of child-specific class I sensitization.

show abstract

Section: Hla-antibody Analysis and Assignment As Child-specificmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Frequency and Determinants of Pregnancy-Induced Child-Specific Sensitization

Hönger

Fornaro

Granado

et al. 2013

American Journal of Transplantation

View full text Add to dashboard Cite

show abstract

“…14 In the case just described, the 2 HLA-antibody-positive donors were men without a history of alloimmunization. Such antibodies in non-alloexposed individuals have already been reported 15 and may represent an immune response to environmental antigen exposure inducing HLA cross-reactive antibodies, termed "natural" antibodies. 16,17 Among the 18 specificities found in Donor …”

Section: Discussionmentioning

confidence: 95%

Fatal peri-operative hyperacute graft rejection during heart transplantation related to infusion of red blood cell concentrate

Kennel

Mattei²,

Vanhuyse³

et al. 2012

The Journal of Heart and Lung Transplantation

View full text Add to dashboard Cite

“…140 Further testing to determine the specificity of HLA antibodies (Class A, B, C, DR, DQ, and DP) was performed using the One Lambda single-antigen bead assays; the assay cutoffs for a positive result were set at a median fluorescence intensity of greater than 2500 for Class I and greater than 1500 for Class II. 141 …”

Section: The Domestic Research Program– Major Findingsmentioning

confidence: 99%

The National Heart, Lung, and Blood Institute Retrovirus Epidemiology Donor Studies (Retrovirus Epidemiology Donor Study and Retrovirus Epidemiology Donor Study-II): Twenty Years of Research to Advance Blood Product Safety and Availability

Kleinman

King

Busch

et al. 2012

Transfusion Medicine Reviews

Self Cite

View full text Add to dashboard Cite

The Retrovirus Epidemiology Donor Study (REDS), conducted from 1989–2001, and the Retrovirus Epidemiology Donor Study-II (REDS-II), conducted from 2004–2012, were National Heart Lung and Blood Institute (NHLBI) funded multicenter programs focused on improving blood safety and availability in the United States. REDS-II also included international study sites in Brazil and China. The three major research domains of REDS/REDS-II have been infectious disease risk evaluation, blood donation availability, and blood donor characterization. Both programs have made significant contributions to transfusion medicine research methodology by the use of mathematical modeling, large-scale donor surveys, innovative methods of repository sample storage, and establishing an infrastructure that responded to potential emerging blood safety threats such as XMRV. Blood safety studies have included protocols evaluating epidemiologic and/or laboratory aspects of HIV, HTLV I/II, HCV, HBV, WNV, CMV, HHV-8, B19V, malaria, CJD, influenza, and T. cruzi infections. Other analyses have characterized: blood donor demographics, motivations to donate, factors influencing donor return, behavioral risk factors, donors’ perception of the blood donation screening process, and aspects of donor deferral. In REDS-II, two large-scale blood donor protocols examined iron deficiency in donors and the prevalence of leukocyte antibodies. This review describes the major study results from over 150 peer-reviewed articles published by these two REDS programs. In 2011, a new seven year program, the Recipient Epidemiology and Donor Evaluation Study-III (REDS-III), was launched. REDS-III expands beyond donor-based research to include studies of blood transfusion recipients in the hospital setting, and adds a third country, South Africa, to the international program.

show abstract

Identification of specificities of antibodies against human leukocyte antigens in blood donors

Abstract: Background-Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related mortality. Blood centers are implementing TRALI risk reduction strategies based on screening apheresis donors for antibodies to human leukocyte antigens (HLA).

Cited by 44 publications

References 21 publications

Frequency and Determinants of Pregnancy-Induced Child-Specific Sensitization

Frequency and Determinants of Pregnancy-Induced Child-Specific Sensitization

Fatal peri-operative hyperacute graft rejection during heart transplantation related to infusion of red blood cell concentrate

The National Heart, Lung, and Blood Institute Retrovirus Epidemiology Donor Studies (Retrovirus Epidemiology Donor Study and Retrovirus Epidemiology Donor Study-II): Twenty Years of Research to Advance Blood Product Safety and Availability

Contact Info

Product

Resources

About