Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis

Hambal, Muhammad; Admi, Masda; Safika, Safika; Sari, Wahyu Eka; Ferasyi, Teuku Reza; Dasrul, Dasrul; Balqis, Ummu; Darmawi, Darmawi

doi:10.14202/vetworld.2019.1540-1545

Cited by 6 publications

(9 citation statements)

References 35 publications

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“…Infectious disturbance may affect the reproductive performance of the cattle's. In support of this hypothesis, various authors explained that due to the ubiquitousness of the bacteria, there are multiple potential sources of preparation infection [11], [12], [13], [14]. In confirmation of our previous study of Escherichia fergusonii [13] and Staphylococcus species [14], both bacteria were found in the preputial swabs of Aceh cattle.…”

Section: Introductionsupporting

confidence: 80%

Phylogenetic Tree 16S rRNA Gene of Acinetobacter soli Isolated from the Prepuce of Aceh Cattle

Admi

Darmawi

Ferasyi

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: In the pre-seed area of healthy Aceh cattle, it is possible to be contaminated with pathogenic bacteria that can interfere with the reproductive system. This study is needed to identify these pathogenic bacteria using a molecular approach, in an effort to prevent infection. AIM: The aim of the present study was to construct phylogenetic tree relationships of Acinetobacter soli identified in the preputial area of Aceh cattle by molecular analysis using 16S rRNA gene sequencing. MATERIALS AND METHODS: A total of 75 preputial specimens were obtained from Indrapuri’s Breeding and Forages Center of Aceh Cattles, Indrapuri district, Banda Aceh, Indonesia. The samples were processed for culture using standard conventional methods. The extraction of genomic DNA and the amplification of the 16S rRNA gene were assayed using polymerase chain reaction. A phylogenetic tree was constructed using distance matrices using the neighbor-joining model of the molecular evolutionary genetic analysis software 6.1 software. RESULTS: The results showed that of 75 preputial swab samples, 18 (24%) were positive for A. soli isolates. There was a 100% sequence similarity to A. soli prototype strain B1 and a 99% similarity to Acinetobacter parvus prototype strain LUH4616, Acinetobacter baylyi strain B2, A. venetianus strain ATCC 31012, as well as a 99% similarity to Acinetobacter baumannii strain DSM 30007, the strain ATCC 19606, and the strain JCM 6841, respectively. We concluded that A. soli-positive presentation in the preparation of Aceh cattle has 100% sequence similarity of 16S rRNA with A. soli strain B1. CONCLUSIONS: The conclusion of this study is that, based on the construction of a phylogenetic tree, it shows that 24% of the bacterial isolate is related to A. soli. It is essential to conduct a regular survey for bacterial contamination and to increase worker awareness and education about hygiene standards.

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Section: Introductionsupporting

confidence: 80%

Phylogenetic Tree 16S rRNA Gene of Acinetobacter soli Isolated from the Prepuce of Aceh Cattle

Admi

Darmawi

Ferasyi

et al. 2022

Open Access Maced J Med Sci

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“…Later, culture-dependent studies were conducted to isolate the bacteria responsible for bull infertility [42] and this technique was also used in more recent studies of the microbiological evaluation of frozen semen samples [43]. During the past few years, the identification of individual bacteria from preputial mucosa [44,45] and bull semen [46] was performed with metagenomic methods. Interestingly, there were no significant correlations between the preputial microbial community of bulls of different ages, breed, diet or co-housing [47].…”

Section: Discussionmentioning

confidence: 99%

Identification of Bull Semen Microbiome by 16S Sequencing and Possible Relationships with Fertility

et al. 2021

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Reports on the use of 16S sequencing for the identification of bacteria in healthy animals are lacking. Bacterial contamination of bull semen can have a negative effect on the sperm quality. The aims of this study were threefold: to identify bacteria in the semen of healthy bulls using 16S sequencing; to investigate the differences in the bacterial community between individual bulls; and to establish if there was a relationship between the bacteria isolated and bull fertility. Semen from 18 bulls of known fertility was used for the DNA extraction and 16S sequencing; 107 bacterial genera were identified. The differences in the amplicon sequence variants (ASVs) and the numbers of genera between bulls were noted. Negative correlations (p < 0.05) between several bacterial genera with Curvibacter, Rikenellaceae RC9-gut-group and Dyella spp. were seen. Other negatively correlated bacteria were Cutibacterium, Ruminococcaceae UCG-005, Ruminococcaceae UCG-010 and Staphylococcus, all within the top 20 genera. Two genera, W5053 and Lawsonella, were enriched in bulls of low fertility; this is the first time that these bacteria have been reported in bull semen samples. The majority of the bacteria were environmental organisms or were species originating from the mucous membranes of animals and humans. The results of this study indicate that differences in the seminal microbiota of healthy bulls occur and might be correlated with fertility.

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“…These isolates were collected in 2019 from nasal swabs of swine in Henan province/China and were cultured on Staphylococcus chromogenic medium. After incubation at 37°C for 24–36 h, colonies were selected for 16S rRNA sequencing as described previously ( 32 ). Staphylococcus aureus RN4220 served as recipient strain in electrotransformation experiments.…”

Section: Methodsmentioning

confidence: 99%

Novel Tet(L) Efflux Pump Variants Conferring Resistance to Tigecycline and Eravacycline in Staphylococcus Spp.

Wang

Schwarz

et al. 2021

Microbiol Spectr

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Tigecycline and eravacycline are both important last-resort broad spectrum antimicrobial agents. The presence of novel Tet(L) efflux pump variants conferring the resistance to tigecycline and eravacycline in Staphylococcus spp. and its potential transmission to S. aureus will compromise the efficacy of tigecycline and eravacycline treatment for S. aureus associated infection in vivo and may lead to clinical treatment failure.

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Identification of Staphylococcus species isolated from preputium of Aceh cattle based on 16S rRNA gene sequences analysis

Cited by 6 publications

References 35 publications

Phylogenetic Tree 16S rRNA Gene of Acinetobacter soli Isolated from the Prepuce of Aceh Cattle

Phylogenetic Tree 16S rRNA Gene of Acinetobacter soli Isolated from the Prepuce of Aceh Cattle

Identification of Bull Semen Microbiome by 16S Sequencing and Possible Relationships with Fertility

Novel Tet(L) Efflux Pump Variants Conferring Resistance to Tigecycline and Eravacycline in Staphylococcus Spp.

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