Identification of stereoisomeric benzo[a]pyrene tetrols by reversed-phase liquid chromatography coupled semi-ondashline to fluorescence line-narrowing spectroscopy

Kok, S.J.; Posthumus, R.; Bakker, I.; Gooijer, C.; Brinkman, U.A.Th.; Velthorst, N.H.

doi:10.1016/0003-2670(94)00463-v

Cited by 28 publications

(23 citation statements)

References 24 publications

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“…(±)-cis-and (±)-trans-anti-BP-tetrol [36]). For the cis and trans forms, the S 1 vibrations are quite different and they are easily distinguished in FLNS [33], but, as expected, the spectra of the optical isomers are identical for the unbound ligands (See Fig. 4, top spectra).…”

Section: Trends Inmentioning

confidence: 67%

“…For BP-tetrol, the stereochemistry of its four hydroxyl groups attached to the saturated ring (cis vs. trans configurations) has a major effect on the FLNS spectrum [33] and an influence of Hbonding solvents can be expected. In the case of BP-6-N7Gua, the only H-bonding atoms are located on the guanine moiety and any effect on the fluorophore from H-bonding is expected to be very minor.…”

Section: Pah Binding To Antibodiesmentioning

confidence: 99%

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Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry

Ariese¹,

Bader²,

Gooijer³

2008

TrAC Trends in Analytical Chemistry

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Fluorescence line-narrowing spectroscopy (FLNS) is a cryogenic technique that can be used to obtain high-resolution fluorescence spectra. The technique has mainly been used for fingerprint identification. However, this review focuses on the trend to use FLNS for probing purposes: obtaining information on interactions with the local environment or determining conformations.We briefly discuss the basics of FLNS, solute-matrix interactions and important instrumental aspects. We describe examples from various bioanalytical fields: DNA adducts of the aromatic carcinogen benzo[a]pyrene (BP); binding of BP metabolites to monoclonal antibodies or the estrogen receptor; FLNS studies of porphyrin-containing proteins; and, photosynthetic systems.As an illustration from the field of environmental chemistry, we discuss the use of luminescence line-narrowing spectroscopy -a special mode of FLNS -to study the complexation of lanthanides to humic substances. ª

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Section: Trends Inmentioning

confidence: 67%

Section: Pah Binding To Antibodiesmentioning

confidence: 99%

Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry

Ariese¹,

Bader²,

Gooijer³

2008

TrAC Trends in Analytical Chemistry

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“…5. Detection limits were at the fmole level [98]. With selective excitation, e.g., 364 nm, the FLN spectra of the trans-and cis-stereoisomers of the antiand syn-BP tetrols are clearly distinguishable at 12 K. The numbers in Fig.…”

Section: Tlc and Flnsmentioning

confidence: 99%

Fluorescence line-narrowing detection in chromatography and electrophoresis

Jankowiak¹,

Roberts²,

Small³

2000

Electrophoresis

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“…These vibrational states are characteristic of a specific molecule, just as those in the ground electronic state, recorded in infrared spectroscopy. With FLN, even stereo-isomers can be distinguished [83]. (For further theoretical considerations regarding this technique, see [84][85][86]).…”

Section: Ce With Fluorescence Line-narrowing Detectionmentioning

confidence: 99%

Analyte identification in capillary electrophoretic separation techniques

et al. 1998

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A review on applications of on-line hyphenation in capillary electrophoresis and capillary electrochromatography for the identification of migrating analytes is presented. There is an urgent need for unambiguous analyte identification by combining spectral information and observed migration times, because the parameters influencing the migration times and separation efficiencies in these separation techniques are not easily controlled, especially when real samples containing unknown interferences have to be analyzed. The spectrometric techniques covered here are ultraviolet and visible radiation (UV/Vis) absorption, fluorescence including fluorescence line-narrowing spectroscopy, Raman spectroscopy, nuclear magnetic resonance and mass spectrometry. Attention is essentially confined to literature reports in which the extra information provided by the detector is really used for identification purposes, especially in real-life samples, while the interfacing as such and analyte detectabilities in standard solutions are only briefly discussed. This article covers an extensive fraction of the literature published on this topic until the beginning of 1998.

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Identification of stereoisomeric benzo[a]pyrene tetrols by reversed-phase liquid chromatography coupled semi-ondashline to fluorescence line-narrowing spectroscopy

Cited by 28 publications

References 24 publications

Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry

Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry

Fluorescence line-narrowing detection in chromatography and electrophoresis

Analyte identification in capillary electrophoretic separation techniques

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