Identification of steroidogenic cell subpopulations in the ovary of the newly hatched chicken

Pedernera, E.; Gómez, Y.; Velázquez, P.N.; Juárez-Oropeza, Marco Antonio; Pliego, Margarita González del

doi:10.1016/0016-6480(88)90306-1

Cited by 12 publications

(6 citation statements)

References 32 publications

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“…S3 oocytes are always observed with some direct contact to the cortex/medulla boundary. In chicken ovaries, granulosa cells of small follicles are steroidogenically inactive (Nitta et al, 1993) and steroidogenic cells recruit to theca layers from the medulla (Narbaitz and Derobert, 1968;Pedernera et al, 1988;Sekido and Lovell-Badge, 2007). The direct connections of PAMS-positive fibers of the medulla and those of the theca layers of S3 oocytes supports the hypothesis that the recruitment of steroidogenic cells into the alligator cortex layer is similar to that observed in chickens (Moore et al, 2008).…”

Section: Discussionsupporting

confidence: 70%

Posthatching development of Alligator mississippiensis ovary and testis

Moore

Hamlin

Botteri

et al. 2009

Journal of Morphology

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We investigated ovary and testis development of Alligator mississippiensis during the first five months post-hatch. To better describe follicle assembly and seminiferous cord development, we employed histochemical techniques to detect carbohydrate-rich extracellular matrix components in one-week, one-month, three-month, and five-month-old gonads. We found profound morphological changes in both ovary and testis. During this time, oogenesis progressed up to diplotene arrest and meiotic germ cells increasingly interacted with follicular cells. Concomitant with follicles becoming invested with full complements of granulosa cells, a periodic acid Schiff’s (PAS)-positive basement membrane formed. As follicles enlarged and thecal layers were observed, basement membranes and thecal compartments gained periodic acid-methionine silver (PAMS)-reactive fibers. The ovarian medulla increased first PAS- and then PAMS-reactivity as it fragmented into wide lacunae lined with low cuboidal to squamous epithelia. During this same period, testicular germ cells found along the tubule margins were observed progressing from spermatogonia to round spermatids located within the center of tubules. Accompanying this meiotic development, interstitial Leydig cell clusters become more visible and testicular capsules thickened. During the observed testis development, the thickening tunica albuginea and widening interstitial tissues showed increasing PAS- and PAMS-reactivity. We observed putative inter-sex structures in both ovary and testis. On the coelomic aspect of testes were cell clusters with germ cell morphology and at the posterior end of ovaries, we observed “medullary rests” resembling immature testis cords. We hypothesize laboratory conditions accelerated gonad maturation due to optimum conditions, including nutrients and temperature. Laboratory alligators grew more rapidly and with increased body conditions compared to previous measured, field-caught animals. Additionally, we predict the morphological maturation observed in these gonads is concomitant with increased endocrine activities.

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Section: Discussionsupporting

confidence: 70%

Posthatching development of Alligator mississippiensis ovary and testis

Moore

Hamlin

Botteri

et al. 2009

Journal of Morphology

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“…By 2 months, these steroidogenic cells have incorporated into the thecal layer of developing follicles (Narbaitz and DeRobertis, 1968;Pedernera et al, 1988). A similar ontogeny of steroidogenic cells also is observed in the lizard Calotes versicolor (Doddamani, 1994) where histochemistry shows 3b-hydroxysteroid dehydrogenase activity beginning after sexual differentiation only in the medulla until 1 month after hatching when activity was also observed in the cortex concomitant with robust folliculogenesis.…”

Section: Discussionmentioning

confidence: 71%

“…One population of cells secretes androgens whereas the other is able to aromatize androgen into estrogens (Pedernera et al, 1988). These cell populations are also present in the embryonic chicken medulla and are responsive to gonadotrophins.…”

Section: Discussionmentioning

confidence: 98%

“…In the hatchling chicken ovary, two types of steroidogenic cells, derived from the primary sex cords, are localized in the medulla (Narbaitz and Adler, 1966;Narbaitz and DeRobertis, 1968;Gonzalez-Moran et al, 1985). One population of cells secretes androgens whereas the other is able to aromatize androgen into estrogens (Pedernera et al, 1988). These cell populations are also present in the embryonic chicken medulla and are responsive to gonadotrophins.…”

Section: Discussionmentioning

confidence: 99%

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Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

Moore

Uribe-Aranzábal

Boggs

et al. 2007

Journal of Morphology

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American alligator (Alligator mississippiensis) ovary development is incomplete at hatching. During the months following hatching, the cortical processes of oogenesis started in ovo continues and folliculogenesis is initiated. Additionally, the medullary region of the gonad undergoes dramatic restructuring. We describe alligator ovarian histology at hatching, 1 week, 1 month, and 3 months of age in order to characterize the timing of morphological development and compare these findings to chicken ovary development. At hatching, the ovarian cortex presents a germinal epithelium containing oogonia and a few primary oocytes irregularly scattered between somatic epithelial cells. The hatchling medulla shows fragmentation indicative of the formation of lacunae. By 1 week of age, oocytes form growing nests and show increased interactions with somatic cells, indicative of the initiation of folliculogenesis. Medullary lacunae increase in diameter and contain secretory material in their lumen. At 1 month, nest sizes and lacunar diameters continue to enlarge. Pachytene oocytes surrounded by somatic cells are more frequent. Trabeculae composed of dense irregular connective tissue divide cortical nests. Three months after hatching oocytes in meiotic stages of prophase I up to diplotene are present. The ovary displays many enlarged follicles with oocytes in diplotene arrest, thecal layers, lampbrush chromosomes, and complete layers of follicular cells. The medulla is an elaborated complex of vascularized lacunae underlying the cortex and often containing discrete lymphoid aggregates. While the general morphology of the alligator ovary is similar to that of the chicken ovary, the progression of oogenesis and folliculogenesis around hatching is notably slower in alligators. Diplotene oocytes are observed at hatching in chickens, but not until 3 months in alligators. Folliculogenesis is completed at 3 weeks in chickens whereas it is still progressing at 3 months in alligators.

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“…Similarly, embryonic turtle ovaries express aromatase around the regressing medullary cords and at the cortex/medulla boundary after sex differentiation (Ramsey et al, 2007). In chicken ovaries 2 weeks after hatching, medullary steroidogenic cells migrate toward the cortex and incorporate into the thecal layers of developing follicles (Narbaitz and DeRobertis, '68; Pedernera et al, '88). Whereas in 5 week old chicken ovaries, aromatase is only detected in the thecal cells of developing follicles, granulosa cells do not express aromatase (Oreal et al, 2002; Govoroun et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Altered sex hormone concentrations and gonadal mRNA expression levels of activin signaling factors in hatchling alligators from a contaminated Florida lake

et al. 2010

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Activins and estrogens participate in regulating the breakdown of ovarian germ cell nests and follicle assembly in mammals. In 1994, our group reported elevated frequencies of abnormal, multioocytic ovarian follicles in 6 month old, environmental contaminant-exposed female alligators after gonadotropin challenge. Here, we investigated if maternal contribution of endocrine disrupting contaminants to the egg subsequently alters estrogen/inhibin/activin signaling in hatchling female offspring, putatively predisposing an increased frequency of multioocytic follicle formation. We quantified basal and exogenous gonadotropin-stimulated concentrations of circulating plasma steroid hormones and ovarian activin signaling factor mRNA abundance in hatchling alligators from the same contaminated (Lake Apopka) and reference (Lake Woodruff) Florida lakes, as examined in 1994. Basal circulating plasma estradiol and testosterone concentrations were greater in alligators from the contaminated environment, whereas activin/inhibin βA subunit and follistatin mRNA abundances were lower than values measured in ovaries from reference lake animals. Challenged, contaminant-exposed animals showed a more robust increase in plasma estradiol concentration following an acute follicle stimulating hormone (FSH) challenge compared with reference site alligators. Aromatase and follistatin mRNA levels increased in response to an extended FSH challenge in the reference site animals, but not in the contaminant-exposed animals. In hatchling alligators, ovarian follicles have not yet formed; therefore, these endocrine differences are likely to affect subsequent ovarian development, including ovarian follicle assembly.

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Identification of steroidogenic cell subpopulations in the ovary of the newly hatched chicken

Cited by 12 publications

References 32 publications

Posthatching development of Alligator mississippiensis ovary and testis

Posthatching development of Alligator mississippiensis ovary and testis

Developmental morphology of the neonatal alligator (Alligator mississippiensis) ovary

Altered sex hormone concentrations and gonadal mRNA expression levels of activin signaling factors in hatchling alligators from a contaminated Florida lake

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