Identification of stool miR-135b-5p as a non-invasive diaognostic biomarker in later tumor stage of colorectal cancer

Li, Li; Wang, Aili; Cai, Min; Tong, Minsi; Chen, Fengtao; Huang, Ling

doi:10.1016/j.lfs.2020.118417

Cited by 30 publications

(31 citation statements)

References 28 publications

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“…Key characteristics of all included studies are shown in Table S1 . Sixteen studies were conducted in Asia (16/20), of which nine were from China [ 23 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ], three from Iran [ 34 , 35 , 36 ], two from Japan [ 37 , 38 ], one from Singapore [ 39 ], and one from Korea [ 40 ]. Only one study was carried out in the US [ 41 ], and the remaining studies were conducted in Europe—two from Italy [ 42 , 43 ] and one from Spain [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…Among 20 studies, only 17 performed colonoscopy for all controls, of which 14 (14/17) explicitly defined people with no findings as healthy controls. Male participants were overrepresented in 15 studies, and two studies did not report age and gender distribution of the study participants [ 26 , 33 ]. Nineteen studies (19/20) reported the stage distribution of CRC cases, and two of them included only early-stage cases [ 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…For miRNA normalization, absolute quantitation was applied in five studies for fecal miRNA [ 19 , 23 , 27 , 28 , 29 ]. Although internal control miRNAs varied in the remaining 15 studies, U6 snRNA was most commonly used (8/15) [ 26 , 31 , 32 , 33 , 34 , 35 , 37 , 40 ]; others included miR-16 [ 33 , 36 ], miR-200b-3p [ 41 ], cel-miR-238 [ 30 ], miR-16-3p [ 43 ], miR-1202 [ 39 ], miR-4257 [ 39 ] miR-24 [ 38 ], and miR-378 [ 42 ]. The studies also used different amounts of stool samples (50–500 mg) and kits for miRNA extraction including miRNeasy mini kit (Qiagen) (14/20), mirVanaTM miRNA isolation kit (2/20), TRIzol (2/20), stool RNA extraction kit (Omega) (1/20), and Stool Total RNA purification kit (1/20).…”

Section: Resultsmentioning

confidence: 99%

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Fecal microRNAs, Fecal microRNA Panels, or Combinations of Fecal microRNAs with Fecal Hemoglobin for Early Detection of Colorectal Cancer and Its Precursors: A Systematic Review

Zhao

Zhu

Bhardwaj

et al. 2021

Cancers

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Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer mortality globally. Fecal miRNAs have been suggested to be promising biomarkers for CRC early detection. We aimed to conduct a systematic literature review on the diagnostic performance of fecal miRNA markers for CRC and its precursors. PubMed and Web of Science were searched to retrieve relevant articles published up to 7 December 2021. Information on study design, characteristics of study population, pre-analytics (sample collection, processing, and storage), fecal miRNA extraction and quantification technologies, and diagnostic performance (including sensitivity, specificity, and area under the curve (AUC)) were summarized. Twenty studies reporting on 31 individual miRNAs and 16 miRNA panels (with 2–9 markers) for CRC diagnosis were identified. Substantial heterogeneity existed regarding stool sample collection, processing, storage, and miRNA extraction and normalization. For two individual miRNAs and one miRNA panel, values ≥ 80% were reported for both sensitivity and specificity; however, none of these results were either internally or externally validated. In a study among fecal immunochemical test-positive cases recruited from a true screening setting, better diagnostic performance was identified and internally validated for a combination panel including two miRNAs, fecal hemoglobin level, and patient age and sex, compared with fecal hemoglobin concentration alone. Fecal miRNAs or miRNA panels, possibly in combination with fecal hemoglobin test, may be promising candidates for noninvasive CRC early detection. However, large prospective and well-designed studies in CRC screening cohorts are required to validate promising miRNAs or miRNA panels.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Fecal microRNAs, Fecal microRNA Panels, or Combinations of Fecal microRNAs with Fecal Hemoglobin for Early Detection of Colorectal Cancer and Its Precursors: A Systematic Review

Zhao

Zhu

Bhardwaj

et al. 2021

Cancers

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show abstract

“…MiRNA expression levels were found to be related with the prognosis of human cancers and may serve as biomarkers of tumor prognosis [ 15 – 17 ]. Li et al discovered that stool miR-135b-5p had excellent diagnosis performance in advanced colorectal cancer [ 18 ]. miR-183-5p expression was decreased in GC tissues, suggesting that it may operate as a predictive biomarker [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

MiR-223 Promotes Tumor Progression via Targeting RhoB in Gastric Cancer

Chen

et al. 2022

Journal of Oncology

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Gastric cancer (GC) is among the most prevalent causes of cancer-related death globally. MiR-223 has been implicated in a variety of cellular mechanisms linked to cancer progression. However, the miR-223 expressions and its function in GC are unknown. We discovered that miR-223 expression was raised in GC tissues in comparison with nearby normal tissues in this investigation. Additionally, multiplied miR-223 expression was strongly linked with TNM stage ( p = 0.022 ), live metastasis ( p = 0.004 ),lymph node metastasis ( p = 0.004 ),and Borrmann type and was associated with an unfavorable prognostic for patients with GC. Furthermore, suppressing miR-223 significantly increased cell death and prevented cell migration and invasion in vitro. Additionally, miR-223 silencing decreased tumor development in vivo. Additionally, we discovered that miR-223 enhanced GC development by specifically targeting RhoB. In summary, our findings reveal that miR-223 increases tumor progression in GC by targeting RhoB, suggesting that it could serve to be a potential biomarker for the prediction of the disease.

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“…Specific examples include the role of miR-17-92 and miRNA-92a as potent promoters of angiogenesis and oncogenic activity[ 121 ]. Likewise, miR-135b-5p[ 122 ], miR-30a-5p[ 40 ], miR-150-5p[ 123 ], miR-183-5p[ 124 ], miR-155[ 125 ], miR-497[ 126 ], miR-181b-5p[ 127 ], miR-375[ 128 , 129 ] and the miR-200 family[ 110 , 130 , 131 ] have been shown to be effective markers of cancer progression. The clinical evaluation of EV miRNA cargo provides insightful information into processes involved in the various stages of cancer from detection to metastasis as summarized in Table 1 .…”

Section: Exosomes and Cancer: Role Of Alcohol-mediated Effectsmentioning

confidence: 99%

Role of cell-free network communication in alcohol-associated disorders and liver metastasis

Kuracha¹,

Thomas²,

Tobi³

et al. 2021

WJG

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The aberrant use of alcohol is a major factor in cancer progression and metastasis. Contributing mechanisms include the systemic effects of alcohol and the exchange of bioactive molecules between cancerous and non-cancerous cells along the brain-gut-liver axis. Such interplay leads to changes in molecular, cellular, and biological functions resulting in cancer progression. Recent investigations have examined the role of extracellular vesicles (EVs) in cancer mechanisms in addition to their contribution as diagnostic biomarkers. Also, EVs are emerging as novel cell-free mediators in pathophysiological scenarios including alcohol-mediated gut microbiome dysbiosis and the release of nanosized EVs into the circulatory system. Interestingly, EVs in cancer patients are enriched with oncogenes, miRNA, lipids, and glycoproteins whose delivery into the hepatic microenvironment may be enhanced by the detrimental effects of alcohol. Proof-of-concept studies indicate that alcohol-associated liver disease is impacted by the effects of exosomes, including altered immune responses, reprogramming of stromal cells, and remodeling of the extracellular matrix. Moreover, the culmination of alcohol-related changes in the liver likely contributes to enhanced hepatic metastases and poor outcomes for cancer patients. This review summarizes the numerous aspects of exosome communications between organs with emphasis on the relationship of EVs in alcohol-associated diseases and cancer metastasis. The potential impact of EV cargo and release along a multi-organ axis is highly relevant to the promotion of tumorigenic mechanisms and metastatic disease. It is hypothesized that EVs target recipient tissues to initiate the formation of prometastatic niches and cancer progression. The study of alcohol-associated mechanisms in metastatic cancers is expected to reveal a better understanding of factors involved in the growth of secondary malignancies as well as novel approaches for therapeutic interventions.

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Identification of stool miR-135b-5p as a non-invasive diaognostic biomarker in later tumor stage of colorectal cancer

Cited by 30 publications

References 28 publications

Fecal microRNAs, Fecal microRNA Panels, or Combinations of Fecal microRNAs with Fecal Hemoglobin for Early Detection of Colorectal Cancer and Its Precursors: A Systematic Review

Fecal microRNAs, Fecal microRNA Panels, or Combinations of Fecal microRNAs with Fecal Hemoglobin for Early Detection of Colorectal Cancer and Its Precursors: A Systematic Review

MiR-223 Promotes Tumor Progression via Targeting RhoB in Gastric Cancer

Role of cell-free network communication in alcohol-associated disorders and liver metastasis

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