Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1

Abstract

Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). Fibroblast colony-forming cells (CFU-F) are present exclusively in the STRO-1+ population. Dual-color cell sorting using STRO-1 in combination with antibody to glycophorin A yields a population approximately 100-fold enriched in CFU-F in the STRO-1+/glycophorin A+ population. When plated under long-term BM culture (LTBMC) conditions, STRO-1+ cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. STRO-1+ cells isolated from LTBMC at later times retain the capacity to generate adherent layers with a cellular composition identical to that of the parent cultures. The STRO-1-selected adherent layers are able to support the generation of clonogenic cells and mature hematopoietic cells from a population of CD34+ cells highly enriched in so-called long-term culture-initiating cells. We conclude that antibody STRO-1 binds to BM stromal elements with the capacity to transfer the hematopoietic microenvironment in vitro.

Establishment and characterization of a human telomerase catalytic subunit-transduced fetal bone marrow-derived osteoblastic cell line

“…Consistently, they do not express marrow stromal cell marker STRO-1 and CD105 (Fig. 4), probably distinct from reported marrow stromal cell lines (Kawano et al 2003;Simmons and Torok-Storb, 1991). As expected, the FBMOB-hTERT can dramatically expand CB HSCs/HPCs ex vivo, especially maintaining HSC properties in vitro at least for 8 weeks.…”

Generation of multinuclear tartrate‐resistant acid phosphatase positive osteoclasts in liquid culture of purified human peripheral blood CD34+ progenitors

“…In the near future it will be interesting to investigate whether a subset of circulating CD34 progenitor cells expresses the STRO-1 surface antigen which identifies stromal precursors (Simmons & Torok-Storb, 1991a, b) and to identify the role of the CD34 /STRO-1 subset in osteoclast generation in vitro. Osteoclasts were observed from day 10 to day 14 of culture, when the maturation of the monocyte/macrophage progeny was still incomplete.…”

Single cell-derived clones from human adipose stem cells present different immunomodulatory properties

“…In this respect, one attempt to standardize the phenotypic characterization of MSCs came from the International Society for Cellular bs_bs_banner Clinical and Experimental Immunology ORIGINAL ARTICLE doi: 10.1111/cei.12270 Therapy (ISCT). The ISCT proposed that MSC populations must be positive for at least the following surface markers: cluster of differentiation (CD)44, CD73, CD90 and CD105 [17][18][19][20][21]. Additionally, these cells should lack the expression of haematopoietic antigens such as CD34 and CD45, as well as markers for monocytes, macrophages and B cells [21].…”