Identification of subgroups of bovine respiratory syncytial virus

Abstract

The occurrence of antigenic variation among nine isolates of bovine respiratory syncytial virus (BRSV) was determined by examining their reaction patterns to human respiratory syncytial virus (HRSV) subgroup A and B monoclonal antibodies (MAbs) by enzyme immunoassay and radioimmunoprecipitation with fractionation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis by using MAbs and polyclonal antisera to HRSV and BRSV. Shared epitopes were demonstrated on four of five structural proteins between BRSV and both subgroups A and B of HRSV. The nine isolates of BRSV showed different patterns of reactivity in enzyme immunoassays with panels of MAbs to HRSV subgroups A and B. Major variations in the molecular weights of the P (phosphoprotein) and F (fusion protein) proteins were demonstrated among the BRSV isolates tested. These results suggest that BRSV belongs to a different antigenic grouping than HRSV and that BRSV is composed of two distinct subgroups.

Severe respiratory disease in dairy cows caused by infection with bovine respiratory syncytial virus

“…Cattle exposed to brsv for the first time can be severely affected (Ames 1993), but the disease may also induce virtually no clinical signs in primary infected animals (Van der Poel and others 1993). There are reports of distinct subgroups of brsv (Baker and others 1992, Mallipeddi and Samal 1993, Furze and others 1994), and antigenic variation may affect the virulence and immunogenicity of the virus (Van der Poel and others 1993). Thus, strain variation may explain the lack of signs or the mild course of the disease observed in some primary infected herds.…”

Analysis of Ruminant Respiratory Syncytial Virus Isolates by RNAse Protection of the G Glycoprotein Transcripts

“…12 Using 9 bovine RSV isolates from different geographic areas in the USA, the existence of 2 bovine subgroups was proposed based on the molecular weight of the RSV P protein revealed on a sodium dodecyl sulfate (SDS) gel. 6 Later it was determined that the P protein of RSV has anomalous electrophoretic mobility with respect to its molecular weight. 7 In vitro transcription and translation of human RSV isolate RSN-2 cDNA with a single point mutation revealed that substitutions involving charged amino acids in the carboxy-terminal region had a marked effect on the electrophoretic mobility of the P protein.…”

Infección por virus respiratorio sincitial: aportaciones de una década

“…Los anticuerpos dirigidos frente las proteínas F, N, P y M de cualquiera de estos virus, son capaces de reconocer el virus heterólogo. Las cepas de BVRS también parecen estar compuestas por dos subgrupos antigénicos principales 58 . Además, se han recuperado cepas ovinas y caprinas de VRS, pero el análisis genético sugiere que estas cepas muestran suficiente relación con el BVRS como para pensar que no son virus similares con huéspedes específicos, sino subgrupos antigénicos 59,60 .…”