Malondialdehyde (MDA) is a marker of work fatigue that occurs. Work fatigue often occurs in workers, but workers do not realise that continuous work fatigue causes health problems in the body. The damage that occurs is oxidative stress in the body that produces the end product, MDA. We conducted a cross-sectional study on as many as 96 home industry workers making tiles in Karang Asem, Grobogan, Central Java, Indonesia. We identified individual factors, individual habitual factors, occupational factors, and work environment factors during the study period and correlated these factors with workers’ MDA levels. MDA is measured using an ELISA reader. Data analysis used the Rank-Spearman correlation test. We identified that the mean value of MDA is 8.66. The analysis correlation test between factors with MDA explained age (p = 0.001), BMI (p = 0.922), sleep quality (p = 0.000), working period (p = 0.390), workload (p = 0.002), length of work (p=0.776), awkward posture (p = 0.013), and heat stress (p = 0.040). There was a significant correlation between age, sleep quality, workload, awkward posture, and heat stress with workers’ MDA levels. Future research should examine other factors related to MDA levels.