Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Tanweer, Fatah A.; Rafii, Mohd Y.; Sijam, Kamaruzaman; Harun, Abdul Rahim; Ahmed, Fahim; Ashkani, Sadegh; Latif, Mohammad Abdul

doi:10.1007/s13313-015-0380-5

Cited by 7 publications

(11 citation statements)

References 36 publications

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“…They were both located on chromosome 11 with 149 and 129 bp, respectively. These results agree with the findings by Tanweer et al (2015a), who identified the Pi-kh gene using RM206. Likewise, Hasan et al (2015) and Ashkani et al (2013) identified Pi-7(t) using RM5961 and PS2 as the donor parent.…”

Section: Discussionsupporting

confidence: 92%

“…In Malaysia, inheritance studies have been performed using local isolates (Rahim et al, 2012), including BC 2 F 3 and BC 2 F 5 advanced backcrosses (Oryza rufipogon x MR219). Four dominant genes at different loci were identified against the most virulent pathotype of M. oryzae Tanweer et al, 2015a). In addition, Ashkani et al (2015) reported an epistatic effect in a study including two different races of fungus.…”

Section: Introductionmentioning

confidence: 97%

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Genetic analysis and identification of SSR markers associated with rice blast disease in a BC2F1 backcross population

et al. 2017

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ABSTRACT. Rice (Oryza sativa L.) blast disease is one of the most destructive rice diseases in the world. The fungal pathogen, Magnaporthe oryzae, is the causal agent of rice blast disease. Development of 2 N. Hasan et al. Genetics and Molecular Research 16 (1): gmr16019280 resistant cultivars is the most preferred method to achieve sustainable rice production. However, the effectiveness of resistant cultivars is hindered by the genetic plasticity of the pathogen genome. Therefore, information on genetic resistance and virulence stability are vital to increase our understanding of the molecular basis of blast disease resistance. The present study set out to elucidate the resistance pattern and identify potential simple sequence repeat markers linked with rice blast disease. A backcross population (BC 2 F 1 ), derived from crossing MR264 and Pongsu Seribu 2 (PS2), was developed using marker-assisted backcross breeding. Twelve microsatellite markers carrying the blast resistance gene clearly demonstrated a polymorphic pattern between both parental lines. Among these, two markers, RM206 and RM5961, located on chromosome 11 exhibited the expected 1:1 testcross ratio in the BC 2 F 1 population. The 195 BC 2 F 1 plants inoculated against M. oryzae pathotype P7.2 showed a significantly different distribution in the backcrossed generation and followed Mendelian segregation based on a single-gene model. This indicates that blast resistance in PS2 is governed by a single dominant gene, which is linked to RM206 and RM5961 on chromosome 11. The findings presented in this study could be useful for future blast resistance studies in rice breeding programs.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 97%

Genetic analysis and identification of SSR markers associated with rice blast disease in a BC2F1 backcross population

et al. 2017

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“…This Chi-square result obtained for blast resistance loci was in line with the result reported by Tanweer et al [54] who recorded foreground marker segregation ratio of 53:106:41 and 55:107:38 for RM208 and RM206 respectively which fitted into the 1:2:1 segregation ratio expected from Mendel studies. Also, Miah et al [15] reported a segregation ratio of 52:127:74 (RM8225) and 54:132:67 (RM6836) which did not differ significantly from the expected Mendelian ratio of 1:2:1.…”

Section: Discussionsupporting

confidence: 91%

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

et al. 2019

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Bacterial leaf blight caused by Xanthomonas oryzae pv oryzae (Xoo) and blast caused by Magnaporthe oryzae are major diseases responsible for significant yield loss in rice production across all rice growing regions. Host plant resistance has been advocated as a sustainable means of guarding against the diseases. This experiment was conducted with the aim to introgress multiple resistance genes against bacterial leaf blight and blast diseases through marker-assisted backcross breeding. Two dominant (Xa4 and Xa21) and two recessive (xa5 and xa13) Xoo resistance genes were introgressed into a high yielding Malaysian rice variety Putra-1 with genetic background of three blast resistance (Piz, Pi2 and Pi9) genes. Eight polymorphic tightly linked functional and SSR markers were used for foreground selection of target genes. Seventy nine polymorphic SSR markers were used in background selection. The plants were challenged at initial stage of breeding and challenged again at BC2F2 with the most virulent Malaysian pathotypes of Xoo (P7.7) and Magnaporthe oryzae (P7.2) to test their resistance. Results obtained from foreground marker segregation analysis at BC1F1 and BC2F1 showed that the marker polymorphism both fitted into the Mendel’s single gene segregation ratio of 1:1 for both Xoo and blast resistance. At BC2F2, results indicated that foreground marker polymorphism fitted into the expected Mendelian ratio of 1:2:1 for blast resistance only. Marker-assisted background selection revealed high percentage of recurrent parent genome recovery (95.9%). It was concluded that the inheritance of blast resistance in the introgressed lines was mainly due to single gene action while the inheritance of Xoo resistance was substantially due to single nuclear gene action. The incorporation of four bacterial leaf blight and three blast resistance genes (Xa4 + xa5 + xa13 + Xa21; Pi9 + Pi2 + Piz) in the newly developed lines would provide for broad spectrum and durable resistance against the two major diseases studied.

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“…More than 100 race specific blast resistance genes have been identified up to date in rice [6,7], and hundreds of quantitative trait loci (QTL) associated with blast resistance have been mapped [8]. Molecular markers linked to these genes and loci have been widely reported and used for assisted breeding of blast resistance [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Epistasis and Quantitative Resistance to Pyricularia oryzae Revealed by GWAS in Advanced Rice Breeding Populations

et al. 2020

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Rice blast caused by Pyricularia oryzae is a major rice disease worldwide. Despite the detailed knowledge on major resistance genes available to date, little is known about how these genes interact with quantitative blast resistance loci and with the genetic background. Knowledge on these interactions is crucial for assessing the usefulness of introgressed resistance loci in breeding germplasm. Our goal was to identify quantitative trait loci (QTL) for blast resistance in rice breeding populations and to describe how they interact among each other and with the genetic background. To that end, resistance to blast was mapped by genome-wide association study (GWAS) in two advanced rice breeding subpopulations, one made of 305 indica type inbred lines, and the other of 245 tropical japonica inbred lines. The interactions and main effects of blast resistance loci were assessed in a multilocus model. Well known, major effect blast resistance gene clusters were detected in both tropical japonica (Pii/Pi3/Pi5) and indica (Piz/Pi2/Pi9) subpopulations with the GWAS scan 1. When these major effect loci were included as fixed cofactors in subsequent GWAS scans 2 and 3, additional QTL and more complex genetic architectures were revealed. The multilocus model for the tropical japonica subpopulation showed that Pii/Pi3/Pi5 had significant interaction with two QTL in chromosome 1 and one QTL in chromosome 8, together explaining 64% of the phenotypic variance. In the indica subpopulation a significant interaction among the QTL in chromosomes 6 and 4 and the genetic background, together with Piz/Pi2/Pi9 and QTL in chromosomes 1, 4 and 7, explained 35% of the phenotypic variance. Our results suggest that epistatic interactions can play a major role modulating the response mediated by major effect blast resistance loci such as Pii/Pi3/Pi5. Furthermore, the additive and epistatic effects of multiple QTL bring additional layers of quantitative resistance with a magnitude comparable to that of major effect loci. These findings highlight the need of genetic background-specific validation of markers for molecular assisted blast resistance breeding and provide insights for developing quantitative resistance to blast disease in rice.

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Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Cited by 7 publications

References 36 publications

Genetic analysis and identification of SSR markers associated with rice blast disease in a BC<sub>2</sub>F<sub>1</sub> backcross population

Genetic analysis and identification of SSR markers associated with rice blast disease in a BC<sub>2</sub>F<sub>1</sub> backcross population

Marker-Assisted Introgression of Multiple Resistance Genes Confers Broad Spectrum Resistance against Bacterial Leaf Blight and Blast Diseases in PUTRA-1 Rice Variety

Epistasis and Quantitative Resistance to Pyricularia oryzae Revealed by GWAS in Advanced Rice Breeding Populations

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