Identification of Sulfavant A as the First Synthetic TREM2 Ligand Discloses a Homeostatic Response of Dendritic Cells After Receptor Engagement

Abstract

The immune response arises from a fine balance of cellular and molecular mechanisms that provide for surveillance, tolerance, and elimination of dangers as pathogens. Improving the quality of the immune response remains a major goal in immunotherapy and vaccine development. Sulfavant A (SULF A) is a sulfolipid that has shown promising adjuvant activity in a cancer vaccine model. Here we report that SULF A is the first synthetic small molecule binding to the Triggering Receptor Expressed on Myeloid cells-2 (TREM2). The receptor engagement initiates an unconventional maturation of Dendritic cells (DCs) leading to upregulation of the Major Histocompatibility Complex class II (MHC Class II) and costimulatory molecules (CD83, CD86, DC54) without release of T helper type 1 (Th1) or 2 (Th2) cytokines. According to a TREM2 mechanism, this response is mediated by SYK-NFAT axis and is compromised by blockade and gene silencing of the receptor. Activation by SULF A preserved the DC functions to excite the allogeneic T cell response, and induced interleukin-10 (IL-10) release after lipopolysaccharide (LPS) stimulation. These results well support the adjuvant effect of SULF A and offer novel insights into the role of TREM2 in the differentiation of an unprecedented DC phenotype (homeDCs) that contributes to the maintenance of immune homeostasis without compromising lymphocyte activation and immunogenic response. The biological function of SULF-A may be of interest in various physiological and pathological processes involving the immune system.

Antitumor Potential of Immunomodulatory Natural Products

“…The product was tested as adjuvant in an experimental model of tumor vaccine against a murine B16F10 melanoma cell line with very encouraging results [176]. Recent studies on the mechanism of action indicated that SULF A is a ligand of the Triggering Receptor Expressed on Myeloid cells-2 (TREM2) [177]. The TREM2-iduced response is mediated by SYK-NFAT axis and is compromised by blockade and gene silencing of the receptor.…”

“…The TREM2-iduced response is mediated by SYK-NFAT axis and is compromised by blockade and gene silencing of the receptor. Activation by this lipid preserved the DC functions to excite the allogeneic T cell response and promoted interleukin-10 (IL-10) release after lipopolysaccharide (LPS) stimulation [177].…”