Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35

Dausset, Caroline; Bornes, Stéphanie; Miquel, Sylvie; Kondjoyan, Nathalie; Angénieux, Magaly; Nakusi, Laurence; Veisseire, Philippe; Alaterre, Elina; Bermúdez‐Humarán, Luis G.; Langella, Philippe; Engel, Erwan; Forestier, Christiane; Nivoliez, Adrien

doi:10.1038/s41598-020-74027-7

Cited by 12 publications

(6 citation statements)

References 42 publications

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“…Similarly, Dausset et al, 2020 [ 27 ] demonstrated that the supernatant of Lacticaseibacillus rhamnosus Lcr35 inhibited the growth of Candida albicans ATCC 10231, thus reducing the viability by 3 log10 (CFU/mL). This suggests that the presence of metabolites produced by this probiotic exerts an antimicrobial effect.…”

Section: Discussionmentioning

confidence: 99%

Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans

García‐Gamboa¹,

Domínguez-Simi²,

Gradilla‐Hernández

et al. 2022

Antibiotics

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Background: There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by Lacticaseibacillus rhamnosus and Pediococcus acidilactici in combination with inulin-type fructans against Candida albicans. Methods: The growth inhibition of Candida was evaluated using microdilution analysis in 96-well microtiter plates; different concentrations of cell-free supernatants of Lacticaseibacillus rhamnosus and Pediococcus acidilactici were exposed to Candida albicans. The antibiofilm assessment was carried out using the crystal violet staining assay. The short-chain fatty acids were analyzed by gas chromatography. Results: The clinically isolated Candida albicans interacted with supernatants from Lacticaseibacillus rhamnosus and Pediococcus acidilactici and showed significant growth inhibition and antibiofilm formation versus the controls. Lactate and acetic acid were elevated in the supernatants. The results suggest that the supernatants obtained from the synbiotic combinations of Lacticaseibacillus rhamnosus and Pediococcus acidilactici with inulin-type fructans can inhibit the growth and biofilm formation against a clinically isolated Candida albicans strain. Conclusions: These results suggest that synbiotic formulations could be a promising alternative to antifungal drugs in candidiasis therapy.

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Section: Discussionmentioning

confidence: 99%

Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans

García‐Gamboa¹,

Domínguez-Simi²,

Gradilla‐Hernández

et al. 2022

Antibiotics

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“…With this decision made, the next step is to choose a formulation, presumably a cream/ointment for local application or a capsule/tablet for vaginal insertion. These present their own challenges, but there have been studies on lactobacilli in creams [35,36] and two products have been approved for intravaginal instillation in Canada [37][38][39] . One of the strains, Lacticaseibacillus rhamnosus Lcr35, was chosen because of properties believed to be suitable for vaginal application, namely anti-Candida activity described in a series of experimental studies [40] .…”

Section: How To Deliver the Strain(s)?mentioning

confidence: 99%

Perspective: microbial interventions in the urinary tract

Reid¹

2023

Microbiome Res Rep

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Despite multiple advances in medicine, the management of urinary tract infections (UTIs) in women has remained stalled for decades. To prevent the development of symptomatic recurrences, low-dose antibiotics are the mainstay, while alternative approaches have been attempted with limited success. The use of probiotics was first considered forty years ago, and while some promising studies have been published, additional evidence in larger patient groups is needed to recommend specific strains as a primary preventive regimen. Overall, the role of beneficial microbes in reducing the risk of UTI and other urological diseases, such as urolithiasis, remains a target for researchers. The aim of this perspective is to offer a viewpoint on the status of this approach and recommendations for how to develop novel probiotic therapies.

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“…Until now, the molecular mechanisms responsible for the anti-pathogenic activity of L. rhamnosus Lcr35 ® have been little described in the literature. The work of Dausset et al (2020) was interested in the mechanisms of action of Gynophilus, a Live Biotherapetic Product (LBP) containing L. rhamnosus Lcr35 ® and sodium thiosulfate, an excipient of the product which potentiate the anti-Candida activity. Thanks to a volatolomic analysis, the authors thus managed to demonstrate the production of a sulfur metabolite, S-methylthioacetate, with fungicidal activity and whose synthesis by L. rhamnosus Lcr35 ® is strongly induced by sodium thiosulfate (Dausset et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The work of Dausset et al (2020) was interested in the mechanisms of action of Gynophilus, a Live Biotherapetic Product (LBP) containing L. rhamnosus Lcr35 ® and sodium thiosulfate, an excipient of the product which potentiate the anti-Candida activity. Thanks to a volatolomic analysis, the authors thus managed to demonstrate the production of a sulfur metabolite, S-methylthioacetate, with fungicidal activity and whose synthesis by L. rhamnosus Lcr35 ® is strongly induced by sodium thiosulfate (Dausset et al, 2020). Although reaching a milestone in the understanding of the mechanism of action, these results did not allow for the moment to describe the molecular anti Candida albicans mechanisms set up by the native strain of L. rhamnosus Lcr35 ® .…”

Section: Introductionmentioning

confidence: 99%

In vivo investigation of Lcr35® anti-candidiasis properties in Caenorhabditis elegans reveals the involvement of highly conserved immune pathways

Poupet

Rifa²,

Theil³

et al. 2022

Front. Microbiol.

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Lactic acid bacteria, including the microorganisms formerly designated as Lactobacillus, are the major representatives of Live Biotherapeutic Microorganisms (LBM) when used for therapeutic purposes. However, in most cases, the mechanisms of action remain unknown. The antifungal potential of LBM has already been demonstrated using preclinical models (cell cultures, laboratory animals). Understanding their mechanisms of action is strategic for the development of new therapeutics for humans. Here, Caenorhabditis elegans was used as an in vivo model to analyze pro-longevity, anti-aging and anti-candidiasis effects of the LBM Lacticaseibacillus rhamnosus (formerly Lactobacillus rhamnosus) Lcr35®. A high-throughput transcriptomic analysis revealed a specific response of C. elegans depending on whether it is in the presence of the LBM L. rhamnosus Lcr35® (structural response), the yeast Candida albicans (metabolic response) or both (structural and metabolic responses) in a preventive and a curative conditions. Studies on C. elegans mutants demonstrated that the p38 MAPK (sek-1, skn-1) and the insulin-like (daf-2, daf-16) signaling pathways were involved in the extended lifespan provided by L. rhamnosus Lcr35® strain whereas the JNK pathway was not involved (jnk-1). In addition, the anti C. albicans effect of the bacterium requires the daf-16 and sek-1 genes while it is independent of daf-2 and skn-1. Moreover, the anti-aging effect of Lcr35®, linked to the extension of longevity, is not due to protection against oxidative stress (H2O2). Taken together, these results formally show the involvement of the p38 MAP kinase and insulin-like signaling pathways for the longevity extension and anti-Candida albicans properties of Lcr35® with, however, differences in the genes involved. Overall, these findings provide new insight for understanding the mechanisms of action of a probiotic strain with antimicrobial potential.

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Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35

Cited by 12 publications

References 42 publications

Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans

Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans

Perspective: microbial interventions in the urinary tract

In vivo investigation of Lcr35® anti-candidiasis properties in Caenorhabditis elegans reveals the involvement of highly conserved immune pathways

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