Identification of superior Cinnamomum zeylanicum Blume germplasm for future true cinnamon breeding in the world

Liyanage, Nishadi Maduwanthi Neluwa; Bandusekara, B. S.; Kanchanamala, R.W.M.K.; Hathurusinghe, Hathurusinghe Arachchilage Bhagya Madhushani; Rathnayaka, Adasooriya Mudiyanselage Ranhami Wedaralage Shanaka Dilhan; Pushpakumara, D. K. N. G.; Samita, S.; Wijesinghe, K. G. G.; Jayasinghe, G. G.; Liyanage, Wasantha Kumara; Bandaranayake, P. C. G.

doi:10.1016/j.jfca.2020.103747

Cited by 14 publications

(14 citation statements)

References 34 publications

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“…Nevertheless, HPLC and GC-MS analysis suggest all C. sinharajaense accessions and C. verum accessions consist of very similar chemical profiles except the differences in concentrations. Such concentration differences are very common in C. zeylanicum [54].…”

Section: Plos Onementioning

confidence: 95%

Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

et al. 2021

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The genus Cinnamomum consists of about 250 species spread globally. Out of these, C. verum (C. zeylanicum), also known as true cinnamon or Ceylon cinnamon, has gained worldwide attention due to its culinary uses and medicinal values. Sri Lanka is the largest true cinnamon producer in the world and accounts for about 80–90% of global production. Other than the cultivated species, Sri Lankan natural vegetation is home to seven endemic wild species of the genus Cinnamomum. While these are underutilized, proper identification and characterization are essential steps in any sustainable conservation and utilization strategies. Currently, species identification is purely based on morphological traits, and intraspecific diversity has made it more challenging. In this study, all the eight Cinnamomum species found in Sri Lanka, C. capparu-coronde, C. citriodorum C. dubium, C. litseifolium, C. ovalifolium, C. rivulorum, C. sinharajaense, and C. verum were collected in triplicates and identified using typical morphological traits. DNA extracted with the same collection was assessed with universal barcoding regions, rbcL, matK, and trnH-psbA. While no intraspecific sequence differences were observed in C. citriodorum, C. rivulorum, and C. verum, the others had polymorphic sites in one, two, or all regions assessed. Interestingly, two individuals of C. sinharajaense had identical barcodes to the cultivated species C. verum, while the other one had one variable cite in matK region and three cites in trnH-psbA reigon. Further, one C. dubium and one C. capparu-coronde accession each had identical, rbcL, and trnH-psbA sequences while those had only a single nucleotide variation observed in matK region. Overall, the phylogeny of Cinnamomum species found in Sri Lanka could not be completely resolved with DNA barcoding regions studied.

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Section: Plos Onementioning

confidence: 95%

Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

et al. 2021

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“…Generally, gas chromatography is applied to characterize volatile compounds while liquid chromatography for the identification of phenolic compounds [13]. It is documented that cinnamon consists of natural antioxidant, anti-microbial and anti-inflammatory components such as volatile oils, flavonoids, curcuminoids, coumarins, tannins, alkaloids, xanthones, terpenoids, phenolics and other compounds in significant amounts [14,15]. The concentration of volatile compounds in cinnamon essential oil (CNO) mainly depends upon the plant parts (leaves, bark, root, stem) from which it is extracted.…”

Section: Phytochemistry Of Cinnamonmentioning

confidence: 99%

Cinnamon: A Natural Feed Additive for Poultry Health and Production—A Review

Ali

Ponnampalam

Pushpakumara

et al. 2021

Animals

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The increased bacterial resistance to synthetic antibiotics and consumer awareness about the health and food safety concerns have triggered the ban on the use of antibiotic growth promotors (AGPs) in the poultry industry. This situation encouraged the poultry sector and industry to explore safe alternatives to AGPs and focus on developing more sustainable feed management strategies to improve the intestinal health and growth performance of poultry. Consequently, phytogenic feed additives (PFAs) have emerged as natural alternatives to AGPs and have great potential in the poultry industry. In recent years, cinnamon (one of the most widely used spices) has attracted attention from researchers as a natural product with numerous health benefits for poultry. The essential oils in cinnamon, in particular, are of interest because of their antioxidant, anti-microbial, anti-inflammatory, antifungal, and hypocholesterolaemic effects, in addition to their ability to stimulate digestive enzymes in the gut. This review mainly emphasizes the potential impact of cinnamon as a natural feed additive on overall gut health, nutrient digestibility, blood biochemical profile, gene expression, gut microbiota and immune response.

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“…The background of the graph divides the y-axis into very good quality calls (green), reasonable quality (orange), and poor quality (red). The graphs are representative of the forward and reverse reads (Liyanage et al 2020a), our modifications would result in quality RNA from other Cinnamomum species.…”

Section: Resultsmentioning

confidence: 99%

“…While the C. zeylanicum bark consists of cinnamaldehyde, cinnamyl acetate, cinnamic acid, cinnamyl alcohol, and β-Phellandrene (Atish Gursale et al 2010;Zachariah and Leela 2018;Damasceno et al 2019) its leaf consists of eugenol, cinnamaldehyde, cinnamyl acetate, cinnamyl alcohol and linalool (Paranagama et al 2001;Shimna et al 2017;Liyanage et al 2020a). C. zeylanicum possess a diverse chemical profile even in the seedling stage grown in greenhouse conditions (Liyanage et al 2020b).…”

Section: Introductionmentioning

confidence: 99%

A CTAB protocol for obtaining high-quality total RNA from cinnamon (Cinnamomum zeylanicum Blume)

2021

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Cinnamomum zeylanicum Blume is an endemic Sri Lankan species commonly known as Ceylon cinnamon or true cinnamon. It is considered the king of spices in addition to its medicinal benefits. Despite recent scientific evidence on its medicinal properties and the industrial demand, cinnamon breeding and crop improvement are not been improved to the expectation. It is mainly due to the limited availability of the genomic information of cinnamon, linked with technical challenges caused by abundant secondary metabolites in all plant parts. Therefore, obtaining high-quality RNA is the fundamental step of transcriptomic analysis and the gene discovery process of cinnamon. We have optimized a CTAB based protocol for high-quality RNA extraction from different cinnamon tissues at various maturity stages collected from the field. Regular pH around 8 and the presence of Polyvinylpyrrolidone (PVP) in CTAB buffer increased the viscosity of the cinnamon lysate. Adjusting the pH of the lysis buffer to 6-6.5 reduced the viscosity of lysate while chloroform precipitates protein efficiently at the adjusted pH with no phenol. Therefore, this protocol excludes PVP and phenol extraction steps. Nanodrop spectrophotometer, gel electrophoresis, and bioanalyzer readings confirmed the quality of extracted RNA. RNA-seq libraries prepared were sequenced with Illumina Sequencing by synthesis technology and obtained good quality data to be used for transcriptomic analysis.

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Identification of superior Cinnamomum zeylanicum Blume germplasm for future true cinnamon breeding in the world

Cited by 14 publications

References 34 publications

Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

Cinnamon: A Natural Feed Additive for Poultry Health and Production—A Review

A CTAB protocol for obtaining high-quality total RNA from cinnamon (Cinnamomum zeylanicum Blume)

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