Identification of sylvatic Trichinella (T3) in foxes from France

Rosa, G. La; Pozio, Edoardo; Barrat, J; Blancou, Jean

doi:10.1016/0304-4017(91)90087-c

Cited by 10 publications

(4 citation statements)

References 8 publications

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“…In some European countries (Spain, France, Czechoslovakia, Croatia, Serbia, Macedonia, Russia, Bulgaria), the epidemiology of trichinellosis is characterized by 2 different cycles due to Trichinella spiralis and Trichinella britovi, in other countries (Italy, Switzerland) only the second species appears to be present (La Rosa et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Random amplified polymorphic DNA technique for the identification of Trichinella species

et al. 1993

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The random amplified polymorphic DNA (RAPD) technique was successfully used to produce genetic fingerprints distinguishing between Trichinella spiralis and Trichinella britovi. The same patterns were obtained from purified and crude DNA preparations of pooled and single muscle larvae. RAPD fingerprinting was applied to muscle larvae preserved under different conditions and recovered from different hosts. Larvae recovered from fresh and frozen meat and stored at -20 degrees C for a long time or under 70% ethyl alcohol at room temperature for 30 d gave good and reproducible results. Single larvae recovered from a naturally infected wild boar and from a human biopsy gave fingerprints congruent to those obtained from T. britovi reference strains. The results prove that RAPD analysis is a quick method to distinguish between the autochthonous Trichinella species of Central-Southern Europe in less than 1 d after the detection of the infection. If necessary, the biological material can be frozen or stored under 70% ethyl alcohol at room temperature and sent to laboratories able to perform the RAPD analysis. The RAPD technique requires no prior knowledge of the molecular biology of the organism to be investigated and therefore appears to be a promising tool in parasitology for the identification of sibling species.

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Section: Introductionmentioning

confidence: 99%

Random amplified polymorphic DNA technique for the identification of Trichinella species

et al. 1993

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“…vulpes ) 4 France Provence Red fox ( V . vulpes ) Isoenzymatic analysis 2/1912 (0.1%) La Rosa et al (1991) France Country-wide Red fox ( V . vulpes ) RAPD-PCR 7/5457 (0.1%) Pozio et al (1996) Germany Not specified Red fox ( V .…”

Section: Resultsmentioning

confidence: 99%

“…lupus ) 2/85 (2.4%) France Lozère & Haute Marne Red fox ( V . vulpes ) Isoenzymatic analysis 2/1912 (0.1%) La Rosa et al (1991) France Not specified Red fox ( V . vulpes ) Allozyme analysis 2 La Rosa et al (1992) France Southwest and Southeastern Finland Badger ( M .…”

Section: Resultsmentioning

confidence: 99%

From wildlife to humans: The global distribution of Trichinella species and genotypes in wildlife and wildlife-associated human trichinellosis

Malone,

Oksanen,

Mukaratirwa

et al. 2024

International Journal for Parasitology: Parasites and Wildlife

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“…Extremely low (0.00001 to 0.1%) or even negligible prevalence rates have recently been reported e.g. from the UK (Smith et al, 2003), Denmark (Enemark et al, 2000), Germany (Schoffel et al, 1991;Ballek et al, 1992), and very low (0.1 to 5 %) in Sweden , Hungary (Sreter et al, 2003), Belgium (Verkammen et al, 2002), the Netherlands (van der Giessen et al, 1998), France (La Rosa et al, 1991), Spain (Perez-Martin et al, 2000) and Switzerland (Gottstein et al, 1997).…”

Section: Reservoir Surveillancementioning

confidence: 99%

Opinion of the Scientific Panel on biological hazards (BIOHAZ) on the on the “Risk assessment of a revised inspection of slaughter animals in areas with low prevalence of Trichinella”

2005

EFSA Journal

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Identification of sylvatic Trichinella (T3) in foxes from France

Cited by 10 publications

References 8 publications

Random amplified polymorphic DNA technique for the identification of Trichinella species

Random amplified polymorphic DNA technique for the identification of Trichinella species

From wildlife to humans: The global distribution of Trichinella species and genotypes in wildlife and wildlife-associated human trichinellosis

Opinion of the Scientific Panel on biological hazards (BIOHAZ) on the on the “Risk assessment of a revised inspection of slaughter animals in areas with low prevalence of Trichinella”

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