Identification of the 2-tridecanone responsive region in the promoter of cytochrome P450<i>CYP6B6</i>of the cotton bollworm,<i>Helicoverpa armigera</i>(Lepidoptera: Noctuidae)

Li, F.; Liu, X.N.; Zhu, Yu‐Cheng; Ma, Junsheng; Liu, N.; Yang, Jianhua

doi:10.1017/s0007485314000698

Cited by 14 publications

(11 citation statements)

References 34 publications

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“…In the same insect, different plant secondary metabolites can induce different genes. For example, the midgut cytochrome P450 enzymes CYP321A1, CYP9A12, CYP9A14, CYP6AE11, and CYP6B7 of Helicoverpa armigera can be overexpressed in response to gossypol (Liu, Liang, Gao, & Shi, ) but tannic acid, quercetin, and other plant secondary metabolites can only induce the overexpression of CYP6B6 genes (Li et al, ). The results of recent studies showed that A .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and expression patterns of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) heat shock protein genes and their response to host stress

Liú

Dang

Song

et al. 2019

Arch Insect Biochem Physiol

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As a polyphagous insect, little is known at the molecular level about the effects of different host plants on physiological changes in Phenacoccus solenopsis. In this study, four heat shock protein (Hsp) genes (PsHsp60, PsHsp70, PsHsp90, and PsHsp20.7) were identified from the transcriptome of P. solenopsis. Analysis of Hsp expression levels revealed significant differences in Hsp gene expression levels in P. solenopsis fed on different host plants. In host conversion tests, the expression levels of PsHsp90 and PsHsp60 were upregulated after transfer of second instar nymphs from tomato to cotton. The expression levels of PsHsp70 and PsHsp20.7 were, respectively, significantly upregulated at 9 and 48 hr after transfer from tomato to Hibiscus. The results of this study aid molecular characterization and understanding of the expression patterns of Hsp genes during different developmental stages and host transfer of P. solenopsis. K E Y W O R D S expression pattern analysis, host plant, Hsp gene, Phenacoccus solenopsis Arch. Insect Biochem. Physiol. 2019;100:e21536.wileyonlinelibrary.com/journal/arch

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Section: Discussionmentioning

confidence: 99%

Molecular characterization and expression patterns of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) heat shock protein genes and their response to host stress

Liú

Dang

Song

et al. 2019

Arch Insect Biochem Physiol

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“…In the present study, the expression of CYP6B7 in the midgut of H. armigera was found to be significantly induced by 2‐tridecanone. Similar results were also observed in the expression of CYP6B6 after 2‐tridecanone treatment at 10 mg/g for 24 and 48 h in H. armigera (Li et al ., ). To investigate the regulatory mechanism of the induction of CYP6B7 , we cloned the promoter region of CYP6B7 by genome walking and analyzed the promoter activity.…”

Section: Discussionmentioning

confidence: 99%

“…The expression level of CYP6B1 and CYP6B4 could be induced by xanthotoxin treatment, and the response element to xanthotoxin in the promoter of CYP6B1 and CYP6B4 was characterized as an overlapping region, including ecdysone response element (EcRE)/antioxidant response element (ARE)/xenobiotic response element to xanthotoxin (XRE‐xan) and a xenobiotic response element to aryl hydrocarbon receptor (XRE‐AhR) (Petersen et al ., ; Brown et al ., , ; McDonnell et al ., ). In H. armigera , a responsive region between −292 and −154 bp in the promoter of CYP6B6 was responsible for the inducible expression of CYP6B6 by 2‐tridecanone (Li et al ., ). The studies mentioned above revealed the importance of the cis ‐acting element or regulatory element of P450 genes in insects’ adaptability to host plants.…”

Section: Introductionmentioning

confidence: 97%

Identification of the 2‐tridecanonecis‐acting element in the promoter of cytochrome P450CYP6B7inHelicoverpa armigera

Luo

et al. 2017

Insect Science

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The expression level of cytochrome P450 genes in insects can be induced by plant allelochemicals, which is important for insects to adapt to host plants. Cytochrome P450 CYP6B7 has been reported to be involved in pyrethroid insecticide resistance in Helicoverpa armigera, and its transcription level was induced by some inducers. Currently, the regulatory mechanism of the induced expression of CYP6B7 remains unknown, although it is very important for understanding the detoxification mechanism to allelochemicals in host plants. The objective of the present study was to investigate the cis-acting element in the promoter of CYP6B7 mediating the inducible up-regulation of CYP6B7 in H. armigera by 2-tridecanone. The promoter region of CYP6B7 was cloned by genome walking technique and analyzed by transient transfection assay. Progressive 5' deletion of the promoter region of CYP6B7 revealed that the relative luciferase activity of construct -320/+232 could be significantly induced by 2-tridecanone. Further stepwise deletion between -320 and -238 bp found that construct -292/+232 could also be significantly induced by 2-tridecanone, but the adjacent construct -256/+232 could not, suggesting the essential role of the sequence between -292 and -257 bp for 2-tridecanone induction. Nucleotide mutations between -292 and -281 bp had no influence on the induction effect by 2-tridecanone, but nucleotide mutations between -280 and -257 bp significantly decreased the induction effect. These results demonstrated that the cis-acting element for 2-tridecanone induction was between -280 and -257 bp in the promoter of CYP6B7.

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“…CYP6B6 is important for the detoxification of exogenous toxic substances, growth, and developmental process in cotton bollworm (Zhao et al, 2016a). In previous research, we identified a 2-TD responsive region in the CYP6B6 promoter, designated as the HE1 element, using transient transfection and mobility shift assays (Li et al, 2014). Using the yeast one-hybrid method, we subsequently identified candidate regulators that bind to the CYP6B6 HE1 element, and one of these was found to be a previously uncharacterized ADH5 of H. armigera (HaADH5) based on the results of National Center for Biotechnology Information Basic Local Alignment Search Tool (NCBI BLAST) analysis.…”

Section: Introductionmentioning

confidence: 99%

Alcohol dehydrogenase 5 of Helicoverpa armigera interacts with the CYP6B6 promoter in response to 2‐tridecanone

Zhao

Qian

et al. 2019

Insect Science

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Alcohol dehydrogenase 5 (ADH5) is a member of medium‐chain dehydrogenase/reductase family and takes part in cellular formaldehyde and S‐nitrosoglutathione metabolic network. 2‐tridecanone (2‐TD) is a toxic compound in many Solanaceae crops to defend against a variety of herbivory insects. In the broader context of insect development and pest control strategies, this study investigates how a new ADH5 from Helicoverpa armigera (HaADH5) regulates the expression of CYP6B6, a gene involved in molting and metamorphosis, in response to 2‐TD treatment. Cloning of the HaADH5 complementary DNA sequence revealed that its 1002 bp open reading frame encodes 334 amino acids with a predicted molecular weight of 36.5 kD. HaADH5 protein was purified in the Escherichia coli Transetta (pET32a‐HaADH5) strain using a prokaryotic expression system. The ability of HaADH5 protein to interact with the 2‐TD responsive region within the promoter of CYP6B6 was confirmed by an in vitro electrophoretic mobility shift assay and transcription activity validation in yeast. Finally, the expression levels of both HaADH5 and CYP6B6 were found to be significantly decreased in the midgut of 6th instar larvae after 48 h of treatment with 10 mg/g 2‐TD artificial diet. These results indicate that upon 2‐TD treatment of cotton bollworm, HaADH5 regulates the expression of CYP6B6 by interacting with its promoter. As HaADH5 regulation of CYP6B6 expression may contribute to the larval xenobiotic detoxification, molting and metamorphosis, HaADH5 is a candidate target for controlling the growth and development of cotton bollworm.

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Identification of the 2-tridecanone responsive region in the promoter of cytochrome P450CYP6B6of the cotton bollworm,Helicoverpa armigera(Lepidoptera: Noctuidae)

Cited by 14 publications

References 34 publications

Molecular characterization and expression patterns of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) heat shock protein genes and their response to host stress

Molecular characterization and expression patterns of Phenacoccus solenopsis (Hemiptera: Pseudococcidae) heat shock protein genes and their response to host stress

Identification of the 2‐tridecanonecis‐acting element in the promoter of cytochrome P450CYP6B7inHelicoverpa armigera

Alcohol dehydrogenase 5 of Helicoverpa armigera interacts with the CYP6B6 promoter in response to 2‐tridecanone

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