Identification of the actinomycete 16S ribosomal RNA gene by polymerase chain reaction in oral inflammatory lesions

Kuyama, Kayo; Fukui, Kenji; Ochiai, Eriko; Maruyama, Satoshi; Iwadate, Kimiharu; Saku, Takashi; Yamamoto, Hirotsugu

doi:10.1016/j.oooo.2013.06.027

Cited by 16 publications

(10 citation statements)

References 27 publications

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“…Similar to previous articles (22), strain identification was done using fermentative biochemical tests (Vitek); nevertheless, molecular identification (16rRNA) would have allowed recognition of new or cryptic species, especially those included within A. israelii (10,26).…”

Section: Discussionmentioning

confidence: 99%

Treatment of cutaneous actinomycosis with amoxicillin/clavulanic acid

Bonifáz

Tirado‐Sánchez

Calderón

et al. 2016

Journal of Dermatological Treatment

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Section: Discussionmentioning

confidence: 99%

Treatment of cutaneous actinomycosis with amoxicillin/clavulanic acid

Bonifáz

Tirado‐Sánchez

Calderón

et al. 2016

Journal of Dermatological Treatment

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“…These results are due to the anaerobic nature of the bacteria and the difficulty of their isolation from their non-sterile milieu [2,28,29]. Moreover, some molecular methods allowing the identification of the type of Actinomyces are in the process of evaluation (Flurorescence in situ hybridization (FISH), sequencing, real-time polymerase chain reaction (PCR), 16S rRNA gene sequencing, DNA sequencing [30][31][32], and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOF MS)) [33].…”

Section: Discussionmentioning

confidence: 99%

Added-Value of Endometrial Biopsy in the Diagnostic and Therapeutic Strategy for Pelvic Actinomycosis

Carrara

Hervy

Dabi

et al. 2020

JCM

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The particularity of pelvic actinomycosis lies in the difficulty of establishing the diagnosis prior to treatment. The objective of this retrospective bicentric study was to evaluate the pertinence and efficacy of the different diagnostic tools used pre-and post-treatment in a cohort of patients with pelvic actinomycosis. The following data were collected: clinical, paraclinical, type of treatment, and the outcome and pertinence of the two diagnostic methods, bacteriological or histopathological, were evaluated. Twenty-seven women were included, with a pre-treatment diagnosis proposed for 66.7% (n = 18) of them. The diagnosis was established in 13.6% (n = 3) of cases through bacteriological samples, and in 93.8% (n = 15) of cases through histopathological samples, with endometrial biopsy positive in 100% of cases. The treatment was surgical with antibiotics for 55.6% (n = 15) of patients, medical with antibiotic therapy for 40.7% (n = 11) of patients, and surgical without antibiotics for one patient. All patients achieved recovery without recurrence, with a median follow-up of 96 days (4-4339 days). Our study suggested an excellent performance of histopathological analysis, and in particular endometrial biopsy, in the diagnosis of pelvic actinomycosis. This tool allowed early diagnosis and, in some cases, the use of antibiotic therapy alone, making it possible to avoid surgery.

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“…Presumptive identification of these microorganisms is based on their morphology (clumping in liquid culture, white colonies with a domed surface or molar tooth-shaped morphology on solid media, which may become irregular after an extended incubation time), Gram-stain morphology, acid-fast negativity, indole-negativity, and metronidazole-resistance [8,67,73]. Additional (species-level) identification may be performed in tertiary-care centers or anaerobic reference laboratories, using biochemical testing methods (e.g., in-house biochemical panels, API 20A, VITEK 2 ANC card), gas-liquid chromatography, molecular methods (DNA–DNA hybridization, PCR, 16S rRNA gene sequencing, and fluorescence in situ hybridization), and MALDI-TOF MS [51,74,75,76,77,78]. Serology is of no relevance in the diagnosis of actinomycosis [5,21,41].…”

Section: Isolation and Identification Of Actinomyces Spp From CLImentioning

confidence: 99%

Microbiological and Clinical Aspects of Cervicofacial Actinomyces Infections: An Overview

2019

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Similarly to other non-spore-forming Gram-positive anaerobes, members of the Actinomyces genus are important saprophytic constituents of the normal microbiota of humans. Actinomyces infections are considered to be rare, with cervicofacial infections (also known as ‘lumpy jaw syndrome’) being the most prevalent type in the clinical practice. Actinomycoses are characterized by a slowly progressing (indolent) infection, with non-specific symptoms, and additionally, the clinical presentation of the signs/symptoms can mimic other pathologies, such as solid tumors, active Mycobacterium tuberculosis infections, nocardiosis, fungal infections, infarctions, and so on. The clinical diagnosis of actinomycosis may be difficult due to its non-specific symptoms and the fastidious, slow-growing nature of the pathogens, requiring an anaerobic atmosphere for primary isolation. Based on 111 references, the aim of this review is to summarize current advances regarding the clinical features, diagnostics, and therapy of cervicofacial Actinomyces infections and act as a paper for dentistry specialists, other physicians, and clinical microbiologists.

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Identification of the actinomycete 16S ribosomal RNA gene by polymerase chain reaction in oral inflammatory lesions

Cited by 16 publications

References 27 publications

Treatment of cutaneous actinomycosis with amoxicillin/clavulanic acid

Treatment of cutaneous actinomycosis with amoxicillin/clavulanic acid

Added-Value of Endometrial Biopsy in the Diagnostic and Therapeutic Strategy for Pelvic Actinomycosis

Microbiological and Clinical Aspects of Cervicofacial Actinomyces Infections: An Overview

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