Identification of the Active Constituents and Significant Pathways of Bushen Huoxue Decoction for the Treatment of Recurrent Spontaneous Abortion Based on Network Pharmacology

Abstract

Background: To explore Bushen Huoxue Decoction (BHD) mechanism in recurrent spontaneous abortion (RSA).Methods: We predicted and screened the action targets of four Traditional Chinese Medicine representatives by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, and searched the disease targets of RSA through Drugbank, DisGeNET, and TTD databases. According to the obtained drug targets and disease targets, the "drug target-disease target" interaction network was further analyzed and constructed. The core target of BHD for treating RSA was were imported to establish in STRING. GO and KEGG pathway analysis were conducted with the RStudio (ggplot2). Finally, HTR-8/SVneo cells were selected to establish a cell model of oxidative stress, and the network pharmacologic results of BHD-RSA were verified by cell biology, Western blot and qRT-PCR.Results: A total of 73 active compounds were obtained from 1084 ingredients present in the BHD, and 125 genes were closely related to RSA. According to the "drug target-disease target" interaction network analysis, 26 core targets of BHD for treating RSA were finally selected, and 203 biological processes, 10 molecular functions, and 10 cell components were enriched by GO function enrichment. KEGG pathway enrichment related pathways, a total of 19, are mainly associated with a Neuroactive ligand-receptor interaction pathway. Experimental results show that compared with the oxidative stress cell model of RSA, BHD increases the expression of PTGFR, AGTR1 and OXTR mRNA by upregulating the expression of PTGFR, AGTR1 and OXTR protein, which may interfere with the occurrence and development of RSA through Neuroactive ligand-receptor interaction pathway.Conclusion: Combined with the pathological mechanism of RSA and the absence of specific signaling pathways, we hypothesized that the BHD might play a role in tonifying the kidney, strengthening the bone, promoting blood circulation, and removing blood stasis，it also can virtually relieve the symptoms of RSA, and our network pharmacological analysis lays the foundation for future clinical research.These results may help define the possible roles the BHD plays in RSA through Neuroactive ligand-receptor interaction pathway.

Research into the mechanism of intervention of SanQi in endometriosis based on network pharmacology and molecular docking technology

“…[6,7] EMS belongs to the category of Zheng Jia (blood stasis) diseases according to traditional Chinese medicine, where its disease mechanism has been described as "blood stasis" for a long period of time. [8] Traditional Chinese medicine is usually used by Chinese doctors in order to remove "blood stasis" as part of their treatment. [9] Sanqi is referred to as a "Pharmacy."…”