Identification of the bacterial superoxide dismutase (SodM) as plant-inducible elicitor of an oxidative burst reaction in tobacco cell suspension cultures

Watt, Stephen A.; Tellström, Verena; Patschkowski, Thomas; Niehaus, Karsten

doi:10.1016/j.jbiotec.2006.02.022

Cited by 24 publications

(11 citation statements)

References 21 publications

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“…Recently, rhamnolipids derived from Pseudomonas aeruginosa were identified as PAMPs recognized by grapevine (Vitis vinifera; Varnier et al, 2009 Similar to flg22 and elf18, the highly conserved RNA-binding motif RNP-1 of bacterial cold shock proteins (CSPs) acts as a PAMP in Solanaceae via the recognition of the 22-amino acid core of RNP-1 (CSP22; Felix and Boller, 2003). Other proteinaceous PAMPs perceived by plants include the superoxide dismutase SodM (Watt et al, 2006), harpins (Engelhardt et al, 2009), and Nep1 (for necrosis-and ethylene-inducing peptide 1)-like proteins (Qutob et al, 2006).…”

Section: Orphan Pampsmentioning

confidence: 99%

Recent Advances in PAMP-Triggered Immunity against Bacteria: Pattern Recognition Receptors Watch over and Raise the Alarm

2009

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Section: Orphan Pampsmentioning

confidence: 99%

Recent Advances in PAMP-Triggered Immunity against Bacteria: Pattern Recognition Receptors Watch over and Raise the Alarm

2009

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“…campestris (accession no. p53654) [57]. Thus, since the N-terminal similarity of SMc00911 to the GlpE sufurtransferase/rhodanese homology domain and the C-terminal similarity to the chromate-resistance protein domain are both greater than the similarity of this protein to SodM, “SodM-like” may not be the most-appropriate annotation for this ORF.…”

Section: Discussionmentioning

confidence: 99%

A comparative genomics screen identifies a Sinorhizobium meliloti 1021 sodM-like gene strongly expressed within host plant nodules

et al. 2012

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BackgroundWe have used the genomic data in the Integrated Microbial Genomes system of the Department of Energy’s Joint Genome Institute to make predictions about rhizobial open reading frames that play a role in nodulation of host plants. The genomic data was screened by searching for ORFs conserved in α-proteobacterial rhizobia, but not conserved in closely-related non-nitrogen-fixing α-proteobacteria.ResultsUsing this approach, we identified many genes known to be involved in nodulation or nitrogen fixation, as well as several new candidate genes. We knocked out selected new genes and assayed for the presence of nodulation phenotypes and/or nodule-specific expression. One of these genes, SMc00911, is strongly expressed by bacterial cells within host plant nodules, but is expressed minimally by free-living bacterial cells. A strain carrying an insertion mutation in SMc00911 is not defective in the symbiosis with host plants, but in contrast to expectations, this mutant strain is able to out-compete the S. meliloti 1021 wild type strain for nodule occupancy in co-inoculation experiments. The SMc00911 ORF is predicted to encode a “SodM-like” (superoxide dismutase-like) protein containing a rhodanese sulfurtransferase domain at the N-terminus and a chromate-resistance superfamily domain at the C-terminus. Several other ORFs (SMb20360, SMc01562, SMc01266, SMc03964, and the SMc01424-22 operon) identified in the screen are expressed at a moderate level by bacteria within nodules, but not by free-living bacteria.ConclusionsBased on the analysis of ORFs identified in this study, we conclude that this comparative genomics approach can identify rhizobial genes involved in the nitrogen-fixing symbiosis with host plants, although none of the newly identified genes were found to be essential for this process.

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“…Further characterization showed that the eliciting activity was strongly reduced or abolished by heat treatment and digestion with proteases ( Figure 1A), two features that distinguish the activity of Xac from peptidoglycan and LPS, two other, nonproteinaceous, bacterial MAMPs known to be recognized by Arabidopsis (Dow et al, 2000;Gust et al, 2007). A previous report identified superoxide dismutase SodM from either Xanthomonas campestris campestris or Escherichia coli (Watt et al, 2006) as a MAMP recognized by cells of tobacco (Nicotiana tabacum; Watt et al, 2006). However, the Arabidopsis cells used in our study showed no response to SodM (see Supplemental Figure 1A online).…”

Section: Arabidopsis and Other Brassicaceae Detect A Mamp From Xanthomentioning

confidence: 91%

The Receptor-Like Protein ReMAX of Arabidopsis Detects the Microbe-Associated Molecular Pattern eMax from Xanthomonas

et al. 2013

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As part of their immune system, plants have pattern recognition receptors (PRRs) that can detect a broad range of microbeassociated molecular patterns (MAMPs). Here, we identified a PRR of Arabidopsis thaliana with specificity for the bacterial MAMP eMax from xanthomonads. Response to eMax seems to be restricted to the Brassicaceae family and also varied among different accessions of Arabidopsis. In crosses between sensitive accessions and the insensitive accession Shakhdara, eMax perception mapped to RECEPTOR-LIKE PROTEIN1 (RLP1). Functional complementation of rlp1 mutants required gene constructs that code for a longer version of RLP1 that we termed ReMAX (for receptor of eMax). ReMAX/RLP1 is a typical RLP with structural similarity to the tomato (Solanum lycopersicum) RLP Eix2, which detects fungal xylanase as a MAMP. Attempts to demonstrate receptor function by interfamily transfer of ReMAX to Nicotiana benthamiana were successful after using hybrid receptors with the C-terminal part of ReMAX replaced by that of Eix2. These results show that ReMAX determines specificity for eMax. They also demonstrate hybrid receptor technology as a promising tool to overcome problems that impede interfamily transfer of PRRs to enhance pathogen detection in crop plants.

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Identification of the bacterial superoxide dismutase (SodM) as plant-inducible elicitor of an oxidative burst reaction in tobacco cell suspension cultures

Cited by 24 publications

References 21 publications

Recent Advances in PAMP-Triggered Immunity against Bacteria: Pattern Recognition Receptors Watch over and Raise the Alarm

Recent Advances in PAMP-Triggered Immunity against Bacteria: Pattern Recognition Receptors Watch over and Raise the Alarm

A comparative genomics screen identifies a Sinorhizobium meliloti 1021 sodM-like gene strongly expressed within host plant nodules

The Receptor-Like Protein ReMAX of Arabidopsis Detects the Microbe-Associated Molecular Pattern eMax from Xanthomonas

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