Identification of the biochemical lesion produced by α-chlorohydrin in spermatozoa

Toxicological studies of Momordica charantia Linn Seed extracts in Male Mice

“…Reduced glycogen content of the testis observed after the treatment of M. charantia seed extract in the present study may be due to impaired glycolysis (Mohri et al, 1975). Similar observation has been made in the testis of dog treated with solusodine compound obtained from Sapindus trifoliatus (Dixit & Gupta, 1982) and neem oil of Azadirachta indica (Kasturi et al, 2002).…”

A reversible contraceptive action of some 6-chloro-6-deoxy sugars in the male rat

“…The antifertility action of low doses of -chlorohydrin (3-chloropropan-l,2-diol) on the male (Ericsson & Youngdale, 1970) is associated with a marked reduction in the ability of spermatozoa from treated animals to oxidize glucose (Mohri, Suter, Brown-Woodman, White & Ridley, 1975;Edwards, Dacheux & Waites, 1976;Ford, Harrison & Waites, 1977) and it is possible that this may be the primary cause of the antifertility action. With the glycolytic effect in mind, we have examined the action of some chlorinated sugars on the fertility of male rats.…”

The interaction of -chlorohydrin with glycerol kinase

“…It must, therefore, be concluded that achlorohydrin is unlikely to inhibit the metabolic activity of spermatozoa by conversion to achlorohydrin phosphate, although the latter compound is a potent inhibitor of some sperm enzymes (Mohri et al, 1975;Mashford & Jones, 1978).…”

“…In fact, synthetic a-chlorohydrin phosphate has been shown to be an inhibitor of both sperm glyceraldehyde phosphate dehydrogenase and trióse phosphate isomerase (Mohri et al, 1975;Mashford & Jones, 1978). Moreover, high concentrations of glycerol can overcome the inhibitory effect of a-chlorohydrin on ram spermatozoa (Edwards et al, 1976;Brown-Woodman et al, 1978), suggesting competition between the two compounds for the same pathway.…”

“…For instance, a period of preincubation of ram spermatozoa with 0-1-1 mM-a-chlorohydrin is required before an inhibition of glycerol metabolism is noted (Edwards, Dacheux & Waites, 1976). Furthermore, the activity of a number of glycolytic enzymes is substantially reduced when measured in ram spermatozoa which have been incubated with 0-03-300 mM-a-chlorohydrin before extraction, yet these same enzymes are not inhibited by the direct addition of a-chlorohydrin to extracts of unincubated spermatozoa (Mohri, Suter, Brown-Woodman, White & Ridley, 1975; Brown-Woodman, Mohri, Mohri, Suter & White, 1978). A number of acrosomal enzymes is also inhibited after, but not before, incubation with 50-120 mM-a-chlorohydrin (Yang & Srivastava, 1976).…”