Identification of the bZIP Gene Family and Investigation of Their Response to Drought Stress in Dendrobium catenatum

Wang, Peng; Li, Yuxin; Zhang, Tingting; Kang, Yuqian; Li, Wei; Wang, Jian; Yu, Wengang; Zhou, Yang

doi:10.3390/agronomy13010236

Cited by 4 publications

(4 citation statements)

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“…During its life cycle, the growth and development of D. catenatum are frequently threatened by environmental conditions, such as temperature and drought stress [37,40]. A lot of stress-related genes or transcription factors were induced to adapt to these environmental stresses [29,33,34,37,40,53]. CHS is a key enzyme in plant secondary metabolic pathways and is associated with many physiological regulations and abiotic stress resistance in plants [54].…”

Section: Discussionmentioning

confidence: 99%

“…Dendrobium catenatum belongs to the Dendrobium genus of the Orchidaceae family and is increasingly threatened by adverse environmental conditions [29]. Researchers have often focused on the medicinal properties of D. catenatum [30][31][32], but little is known about its stress resistance [33,34]. In this study, the D. catenatum CHS genes were identified, and their full-length genomic sequence was obtained [35].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis of the Chalcone Synthase (CHS) Gene Family in Dendrobium catenatum

Yang,

Zhang,

et al. 2023

Agronomy

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Flavonoids are considered to be critical metabolites in regulating plant responses to abiotic stress. Chalcone synthase (CHS) catalyzes the first key step in the flavonoid biosynthesis pathway. However, there is no in-depth information on the sequence and functional analysis of CHS genes in Dendrobium catenatum. In this study, a total of 14 DcCHS genes were identified, encoding proteins of 349–504 amino acids in length, a protein molecular weight ranging between 39.08 and 56.56 kDa, and isoelectric points from 5.64 to 9.63. The DcCHS proteins were then divided into three groups according to their phylogenetic relationships. The members of each group had similar conserved motifs and gene structures. Quantitative real-time RT-PCR (RT-qPCR) analysis revealed that the DcCHS genes exhibited variable expression patterns in the different plant tissues evaluated. Furthermore, six genes were differentially expressed following exposure to abiotic stresses: DcCHS-6, DcCHS-5/-6, DcCHS-13/-14, and DcCHS-6/-8/-9/-13, which were specifically expressed in response to drought, heat, cold, and salt stress, respectively. This is the first genome-wide analysis of the CHS genes in D. catenatum, and our findings can provide essential information for a better understanding of the function of DcCHS genes, thus facilitating further research on D. catenatum stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the Chalcone Synthase (CHS) Gene Family in Dendrobium catenatum

Yang,

Zhang,

et al. 2023

Agronomy

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“…Gene model (PF00170 and PF07716) of the bZIP TF family were downloaded from the Pfam database (http://pfam.xfam.org/)., The bZIP TF family of A. membranaceus was predicted using the hidden Markov model software HMMER3v3.0 (http://hmmer.janelia.org/) based on the genomic data of A. membranaceus, and the prediction results were further identi ed using the hidden Markov model tool (https://www.ebi.ac.uk/Tools/hmmer/) and NCBI CDD-Search online tool (https//www.Ncbi.nlm.nih.gov/Structure/bwrpsb/bwrpsb.cgi). The physicochemical properties of the bZIP TF family, including protein length, molecular weight, isoelectric point and other indicators, were analyzed using ProtParam (https://web.expasy.org/protparam/) 45,46 .…”

Section: Identi Cation and Physicochemical Property Analysis Of The B...mentioning

confidence: 99%

Characterization of the bZIP Family Transcription Factors in Astragalus membranaceus and Their Expression under Drought Stress

Wang,

Rong,

Shi

et al. 2024

Preprint

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Astragalus membranaceus has important medicinal and ecological values, but with global changes, its growth is often negatively affected by abiotic stresses such as drought stress. The bZIP family transcription factors (TF) play important roles in plant growth and development, and the response to abiotic stresses. However, systematic identification of the bZIP gene family in A. membranaceus and their expressions under drought stress have not been reported. In the present study, genome-wide identification, chromosome localization, phylogenetic analysis, motif analysis and domain prediction of the bZIP family genes in A. membranaceus were performed. A total of 74 bZIP family members were identified in A. membranaceus, which are divided into 13 subfamilies, and members in each subfamily have similar gene structures and conserved motifs. The bZIP family members were unequally distributed on the 9 chromosomes of A. membranaceus. Four bZIP genes were generated via tandem duplication, including Am01G021190 and Am01G021150, and 45 were formed though segmental duplication. Compared with Zea mays and Oryza sativa, the bZIP genes of A. membranaceus have more collinearity with that of Medicago truncatula. The promoter analysis showed that the bZIP transcription factor family was rich in plant hormone response elements and abiotic stress response elements. The analysis of transcriptome data and qRT-PCR indicated that several bZIP TFs including AmZIP46 was involved in the response to drought stress. Further experiments showed that AmbZIP46 is only localized in the nucleus and has transcriptional activation activity. This study provided important data for understanding the biological functions of the bZIP family transcription factors in A. membranaceus.

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“…Therefore, identifying drought-resistant genes from D. catenatum is crucial for improving drought resistance through molecular breeding techniques. Our research group has previously discovered several drought-related transcription factors in D. catenatum [33][34][35][36][37], which serve as candidate genes for studying drought resistance in D. catenatum. Within plants, the CBL-CIPK signaling pathway has a significant role in responding to abiotic stress.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in Dendrobium catenatum and Their Response to Drought Stress

Ren,

Wang,

Zhang

et al. 2023

Horticulturae

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Dendrobium catenatum is a high-value medicinal plant that is predominantly found in high mountain areas, thriving amidst cliffs and rock crevices. However, its wild resources face constant threats from adverse environmental conditions, especially drought stress. Fatty acyl-CoA reductase (FAR) is crucial in plant drought resistance, but there is a lack of research on FAR genes in D. catenatum. In this study, the FAR family genes were identified from the D. catenatum genome. Their genomic characteristics were investigated using bioinformatics techniques, and their expression patterns in different tissues and under 20% PEG8000 conditions mimicking drought stress were analyzed using quantitative real-time RT-PCR (RT-qPCR). Seven DcFAR genes were identified from the D. catenatum genome. The encoded amino acids range between 377 and 587 aa, with molecular weights between 43.41 and 66.15 kD and isoelectric points between 5.55 and 9.02. Based on the phylogenetic relationships, the FAR family genes were categorized into three subgroups, each with similar conserved sequences and gene structures. The cis-acting elements of the promoter regions were assessed, and the results reveal that the DcFAR upstream promoter region contains multiple stress-related elements, suggesting its potential involvement in abiotic stress responses. The RT-qPCR results show distinct expression patterns of DcFAR genes in various plant tissues. It was observed that the expression of most DcFAR genes was upregulated under drought stress. Among them, the expression levels of DcFAR2, DcFAR3, DcFAR5, and DcFAR7 genes under drought stress were 544-, 193-, 183-, and 214-fold higher compared to the control, respectively. These results indicate that DcFAR2/3/5/7 might play significant roles in D. catenatum drought tolerance. This research offers insight into the function of DcFAR genes and provides theoretical support for breeding drought-resistant D. catenatum varieties.

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Identification of the bZIP Gene Family and Investigation of Their Response to Drought Stress in Dendrobium catenatum

Cited by 4 publications

References 72 publications

Genome-Wide Identification and Expression Analysis of the Chalcone Synthase (CHS) Gene Family in Dendrobium catenatum

Genome-Wide Identification and Expression Analysis of the Chalcone Synthase (CHS) Gene Family in Dendrobium catenatum

Characterization of the bZIP Family Transcription Factors in Astragalus membranaceus and Their Expression under Drought Stress

Genome-Wide Identification of Fatty Acyl-CoA Reductase (FAR) Genes in Dendrobium catenatum and Their Response to Drought Stress

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