Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus

Zhang, Fuxian; Guo, Hongbo; Zhang, Jie; Chen, Qingxiu; Qin, Fang

doi:10.1016/j.virol.2017.09.019

Cited by 61 publications

(56 citation statements)

References 68 publications

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“…This pathway is utilized by growth hormones, extracellular fluid, glycosylphosphatidylinositol (GPI)-linked proteins and interleukin-2 to enter the cells. For instance, folic acid that employs clathrin- and caveolae-independent pathway to enter the cells [ 58 , 72 , 75 – 79 ] are conjugated to NPs and polymers used in drug delivery systems and as imaging agents [ 53 , 80 , 81 ]. Macropinocytosis is a type of pinocytosis mechanism in which cells take in high volumes of extracellular fluid by forming a large vesicle (0.5–10 μm) called macropinosomes [ 82 – 85 ].…”

Section: Cellular Uptake Pathways Of Npsmentioning

confidence: 99%

Insight into Cellular Uptake and Intracellular Trafficking of Nanoparticles

2018

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Nanoparticle science is rapidly changing the landscape of various scientific fields and defining new technological platforms. This is perhaps even more evident in the field of nanomedicine whereby nanoparticles have been used as a tool for the treatment and diagnosis of many diseases. However, despite the tremendous benefit conferred, common pitfalls of this technology is its potential short and long-term effects on the human body. To understand these issues, many scientific studies have been carried out. This review attempts to shed light on some of these studies and its outcomes. The topics that were examined in this review include the different possible uptake pathways of nanoparticles and intracellular trafficking routes. Additionally, the effect of physicochemical properties of nanoparticle such as size, shape, charge and surface chemistry in determining the mechanism of uptake and biological function of nanoparticles are also addressed.

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Section: Cellular Uptake Pathways Of Npsmentioning

confidence: 99%

Insight into Cellular Uptake and Intracellular Trafficking of Nanoparticles

2018

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“…In the past decades, it has been proved that many viruses can be internalized via clathrin‐ and caveolae‐mediated endocytosis by different methods. By using specific inhibitors, RNA interference, and dominant negative mutants, it has been indicated that hepatitis C virus, African swine fever virus, hepatitis E virus, and porcine hemagglutinating encephalomyelitis virus enter their host cells via clathrin‐mediated endocytosis, while grass carp reovirus and porcine sapelovirus enter their host cells via caveolae‐mediated endocytosis . In addition to these conventional methods, single‐virus tracking provides many new insights into the study of the virus endocytic mechanism.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of transmissible gastroenteritis virus internalization unraveled by single‐virus tracking in live cells

Wang

et al. 2020

FASEB j.

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Transmissible gastroenteritis virus (TGEV) is a swine enteropathogenic coronavirusthat causes significant economic losses in swine industry. Current studies on TGEV internalization mainly focus on viral receptors, but the internalization mechanism is still unclear. In this study, we used single-virus tracking to obtain the detailed insights into the dynamic events of the TGEV internalization and depict the whole sequential process. We observed that TGEVs could be internalized through clathrin-and caveolae-mediated endocytosis, and the internalization of TGEVs was almost completed within ~2 minutes after TGEVs attached to the cell membrane. Furthermore, the interactions of TGEVs with actin and dynamin 2 in real time during the TGEV internalization were visualized. To our knowledge, this is the first report that single-virus tracking technique is used to visualize the entire dynamic process of the TGEV internalization: before the TGEV internalization, with the assistance of actin, clathrin, and caveolin 1 would gather around the virus to form the vesicle containing the TGEV, and after ~60 seconds, dynamin 2 would be recruited to promote membrane fission.These results demonstrate that TGEVs enter ST cells via clathrin-and caveolaemediated endocytic, actin-dependent, and dynamin 2-dependent pathways. K E Y W O R D Sactin, caveolae, clathrin, dynamin 2, TGEV 4654 | WANG et Al.

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“…GCRV-I is the most widely studied type among the three types of GCRV. It is reported that caveolae/raft-mediated endocytosis is the primary entry pathway for GCRV-I [38] and disruption of clathrin-dependent trafficking results in failure of GCRV-I cellular entry [39]. Thus, the invasion mechanism of GCRV-I remains controversial.…”

Section: Discussionmentioning

confidence: 99%

Grass Carp Reovirus Major Outer Capsid Protein VP4 Interacts with RNA Sensor RIG-I to Suppress Interferon Response

Fan

Liao

et al. 2020

Biomolecules

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Diseases caused by viruses threaten the production industry and food safety of aquaculture which is a great animal protein source. Grass carp reovirus (GCRV) has caused tremendous loss, and the molecular function of viral proteins during infection needs further research, as for most aquatic viruses. In this study, interaction between GCRV major outer capsid protein VP4 and RIG-I, a critical viral RNA sensor, was screened out by GST pull-down, endogenous immunoprecipitation and subsequent LC-MS/MS, and then verified by co-IP and an advanced far-red fluorescence complementation system. VP4 was proved to bind to the CARD and RD domains of RIG-I and promoted K48-linked ubiquitination of RIG-I to degrade RIG-I. VP4 reduced mRNA and promoter activities of key genes of RLR pathway and sequential IFN production. As a consequence, antiviral effectors were suppressed and GCRV replication increased, resulting in intensified cytopathic effect. Furthermore, results of transcriptome sequencing of VP4 stably expressed CIK (C. idella kidney) cells indicated that VP4 activated the MyD88-dependent TLR pathway. Knockdown of VP4 obtained opposite effects. These results collectively revealed that VP4 interacts with RIG-I to restrain interferon response and assist GCRV invasion. This study lays the foundation for anti-dsRNA virus molecular function research in teleost and provides a novel insight into the strategy of immune evasion for aquatic virus.

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Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus

Cited by 61 publications

References 68 publications

Insight into Cellular Uptake and Intracellular Trafficking of Nanoparticles

Insight into Cellular Uptake and Intracellular Trafficking of Nanoparticles

Dynamics of transmissible gastroenteritis virus internalization unraveled by single‐virus tracking in live cells

Grass Carp Reovirus Major Outer Capsid Protein VP4 Interacts with RNA Sensor RIG-I to Suppress Interferon Response

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