Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity

A single point mutation in the novel PvCesA3 gene confers resistance to the carboxylic acid amide fungicide mandipropamid in Plasmopara viticola

“…3). This finding is consistent with the results obtained by Grenville-Briggs et al (2008) and Fugelstad et al (2009). In P. viticola and other members of the oomycetes it remains to be explored how CesA proteins interact together during cellulose biosynthesis.…”

“…Phylogenetic analysis of Plasmopara CesA gene products with published cellulose synthases of various kingdoms revealed that they group as a distinct clade together with the cellulose synthases of Phytophthora species and Saprolegnia monoica, recently published by Fugelstad et al (2009). The oomycete clade is split into four subclades, each representing one of the four CesA genes that have been identified in oomycetes (Fig.…”

The cellulose synthase 3 (CesA3) gene of oomycetes: structure, phylogeny and influence on sensitivity to carboxylic acid amide (CAA) fungicides

“…In oomycetes and plants, the machinery responsible for cellulose formation is a membrane bound enzyme complex (Paredez et al 2006;GrenvilleBriggs et al 2008) probably composed of several distinct cellulose synthase (CesA) subunits (Desprez et al 2007;Persson et al 2007). In oomycetes, up to four CesA encoding genes have been identified in Peronosporales, Pythiales and Saprolegniales (Grenville-Briggs et al 2008;Fugelstad et al 2009;Blum et al 2010b;Levesque et al 2010;Blum et al 2011); and their direct involvement in cellulose biosynthesis was demonstrated using RNA interference in P. infestans (Grenville-Briggs et al 2008). Out of the four CesAs, the CesA3 gene was most strongly expressed during mycelial growth of Phytophthora, Saprolegnia and Pythium species (GrenvilleBriggs et al 2008;Fugelstad et al 2009; indicating that it is of particular importance for the formation of cellulose in oomycetes.…”

Synthesis and Self-Assembly of Cellulose Microfibrils from Reconstituted Cellulose Synthase

“…It consists of bundles of b-1,4-glucan chains typically organized as microfibrillar structures. It is mostly found in plants, bacteria, oomycetes, and green algae (Fugelstad et al, 2009;John et al, 2011;Augimeri et al, 2015). Cellulose is the main structural component of plant cell walls, and is used for several technological applications including manufacturing of paper, textiles, and furniture (McFarlane et al, 2014).…”