Identification of the Chicken Anemia Agent, Reproduction of the Disease, and Serological Survey in the United States

Abstract

An agent with antigenic, physicochemical, and pathological characteristics of chicken anemia agent (CAA) was isolated from broiler chickens and was designated chicken infectious anemia (CIA)-1. CIA-1 was resistant to chloroform treatment and passed through 50-nm-diameter-pore membranes. When inoculated in embryonally bursectomized and/or intact chickens, CIA-1 produced signs and lesions characteristic of CIA: low hematocrit values, pale bone marrow, thymic and bursal atrophy, and enlarged liver. Microscopic lesions were a reflection of macroscopic observations. When injected into 4-week-old chickens CIA-1 induced antibodies against the Cux-1 CAA isolate. In CsCl, CIA-1 had a density of 1.36 g/ml. Antibodies against CAA were found in breeder and commercial chicken flocks from Arkansas, Connecticut, Georgia, Kansas, Maine, Michigan, New York, and Pennsylvania. The age of these flocks ranged from 10 to 78 weeks.

Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens

“…Since then, numerous publications have documented the worldwide occurrence of CAA in broilers (Yuasa et al, 1979(Yuasa et al, , 1983a(Yuasa et al, , 1987Goryo et al, 1987;Engstrom, 1988;Otaki et al, 1988;Vielitz & Landgraf, 1988;Chettle et al, 1989;Goodwin et al, 1989;Rosenberger & Cloud, 1989a;McNulty et al, 1990a). Antibody against CAA was detected in the majority of commercial poultry flocks of egg-laying and meat-type breeds (Yuasa et al, 1985;Braunius, 1988;McNulty et al, 1988McNulty et al, , 1989Goodwin et al, 1990;JfSrgensen, 1990;Lucio et al, 1990). Both vertical (Yuasa & Yoshida, 1983) and horizontal (Yuasa et al, 1979(Yuasa et al, , 1980Rosenberger & Cloud, 1989b;McNulty et al, 1990b) transmission of CAA have been reported, but little is still known on the persistence of CAA and CAA antibody in hens.…”

Identification of chicken infectious anaemia, isolation of the virus and reproduction of the disease in Argentina11

“…Cux-1-infected and control MSB-1 cells were used as positive and negative antigen preparations for the detection of antibodies as described by Lucio et al (1990). Mab 51.3 and RAM FITC were used to examine antigens for CIAV in frozen sections.…”

Production of Polyclonal Antibodies Against Recombinant Vp6 Antigen of Rotavirus

“…These factors make it necessary to develop a diagnostic system that permits the specific detection of rotavirus [5]. Group A rotavirus is formed by three concentric layers of protein in which the intermediate layer is formed by VP6 [6,7] Protein VP6 is the most abundant protein (51 %) and highly conserved with approximately 90 % homology at the amino acid level among group A rotavirus [8]. It is also highly immunogenic rotavirus protein [9].…”