Identification of the Cryptosporidium Isolate from Chickens in Japan by Sequence Analyses

Abstract

ABSTRACT. The Cryptsosporidium isolate from chickens in Japan by Itakura et al. is not yet accurately identified because of several discrepancies in phenotypic features. We attempted to identify this isolate by analyzing the partial sequences of the 18S rRNA, COWP and HSP70 genes. The chicken isolate showed nearly 100% homology in each gene with C. baileyi, but less than 91% homology with C. meleagridis. In addition, these genes were classified into the same cluster with C. baileyi other than C. meleagridis by phylogenetic analysis. From these results, the Cryptosporidium isolate from chickens in Japan is considered to be one of the strains of C. baileyi.

Public health significance of zoonotic Cryptosporidium species in wildlife: Critical insights into better drinking water management

“…Species nameMajor host(s)Site of infectionReferences C. meleagridis Turkey ( Meleagris gallopavo ), Indian ring-necked parrot ( Psittacula kameri ), Red-legged partridge ( Alectoris rufa ), Cocktails ( Nymphicus hollandicus ), Bohemian waxwing ( Bombycilla garrulous ), Rufousturle dove ( Streptopelia orientalis ), Fan-tailed pigeon ( Columba livia ), Chicken ( Gallus gallus ), Quails ( Coturnixcoturnix japonica ), Pekin ducks ( Anas platyrhynchos ), Domestic Pigeons ( Columba livia domestica ), European turtle dove ( Streptopelia turtur ), Red-legged partridge ( Alectoris rufa )IntestineMorgan et al., 2000, Glaberman et al., 2001, Abe and Iseki, 2004, Abe and Makino, 2010, Wang et al., 2010, Qi et al., 2011, Berrilli et al., 2012, Wang et al., 2012, Baroudi et al., 2013, Wang et al., 2014, Koompapong et al., 2014, Maca and Pavlasek, 2015, Reboredo-Fernández et al., 2015 C. baileyi Turkey ( Meleagris gallopavo ), Chicken ( Gallus gallus ), Brown squail ( Synoicus australis ), Cocktails ( Nymphicus hollandicus ), Whooping crane ( Grus vipio ), Grey-bellied bulbul ( Pycnonotus spp. ), Black vulture ( Coragyps atratus ), Saffron finch ( Sicalis flaveola ), Mixed-bred falcons ( Falcorusticolus x Falco cherrug ), Reddy Shelduck ( Tadornaferruginea ), Red-billed leiothrixes ( Leiothrix lutea ), Pekin ducks ( Anas platyrhynchos ), Buffy-fronted seedeater ( Sporophila frontalis ), Javva sparrows ( Padda oryzivora ), Mynas ( Acridotheres tristis ), Zebra finches ( Taeniopygia guttata ), Crested Lark ( Galerida cristana ), Gouldian finch ( Chloebia gouldiae ), Black-billed magpie ( Pica pica ), Ostriches ( Struthio camelus ), Quails ( Coturnixcoturnix japonica ), Red grouse ( Lagopus lagopus scotica ), Red-crowned crane ( Grus japonenis )Cloaca, bursa, tracheaMorgan et al., 2001, Abe and Iseki, 2004, Ng et al., 2006, Huber et al., 2007, Kimura et al., 2004, Nakamura et al., 2009, Abe and Makino, 2010, Wang et al., 2010, Qi et al., 2011, Wang et al., 2012, Baroudi et al., 2013, Baines et al., 2014, Hamidinejat et al., 2014, Wang et al., 2014, Li et al., 2015c, Maca and Pavlasek, 2015 C. galli Chicken ( Gallus gallus ), Finches ( Spermestidae and Fringillidae ), Capercaille ( Tetrao urogallu ), Pine grosbeak ( Pinicola enuncleator ), Turqoise parrots ( Neophema pulchella ), Cuban flamingo ( Phoenicopterus ruber ruber ), Rhinoceros hornbill ( Buceros rhinoceros ), Red-cowled cardinal (P aroaria dominicana ), Zebra finches ( Taeniopygia guttata ), Chocolate parson finches ( Peophila cincta ), Chesnut finches ( Lonchura castaneothorax ), Painted firetali...…”

Cryptosporidium in birds, fish and amphibians

“…Cryptosporidium baileyi is probably the most common avian Cryptosporidium sp., and has been reported in a wide range of avian hosts including black-headed gulls, chickens, cormorants, cranes, a channel-billed toucan, an eastern golden-backed weaver, turkeys, ducks, geese, cockatiels, a brown quail, a grey-bellied bulbul, an ostrich, a red-rumped cacique, a crested oropendola, a red crowned amazon, a rose-ringed parakeet, a grey partridge and mixed-bred falcons , Pavlásek, 1993, Ryan, et al, 2003a, Abe and Iseki, 2004, Jellison, et al, 2004, Kimura et al, 2004, Chvala, et al, 2006Huber et al, 2007;Van Zeeland et al, 2008).…”

Large-scale survey ofCryptosporidiumspp. in chickens and Pekin ducks (Anas platyrhynchos) in Henan, China: prevalence and molecular characterization

“…However, most reports on the prevalence in chickens were published in the 1980s and 1990s, with infection rates ranging from 4.5 to 50% depending on the location and the methods used in detection (de Graaf et al, 1999). In contrast, in the past 10 years there have been only about six reports (Trampel et al, 2000;Kimura et al, 2004;Huber et al, 2007;Soltane et al, 2007;Shemshadi et al, 2010;Smith et al, 2010). Likewise, there are only a few reports (about seven) concerning Cryptosporidium infections in ducks (Mason, 1986;Richter et al, 1994;O'Donoghue, 1995;Morgan et al, 2001;Kuhn et al, 2002;Huber et al, 2007;Amer et al, 2010).…”