Identification of the diterpenoid biosynthesis genes and their expression status in relation to oleoresin yield of masson pine

Mei, Lina; Yan, Youjin; Li, Zhengchun; Ran, Jiaxin; Shen, Luonan; Wu, Rongju; Hou, Qiandong; Shen, Tao; Wen, Xiaopeng; Yang, Zhangqi; Feng, Ying

doi:10.1016/j.indcrop.2021.113827

Cited by 11 publications

(5 citation statements)

References 58 publications

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“…RNA-Seq and iTRAQ methods have extensively been used to identify DEGs and DEPs involved in plant development and secondary metabolism. In recent years, many RNA-Seq studies have been widely conducted to investigate the transcriptional regulation in the resin yield of masson pine [ 36 , 37 , 38 , 39 , 40 ]. However, the application of iTRAQ-based proteomic analysis was relatively rare, so far, no report was available to explore the molecular regulatory mechanisms in resin yield of masson pine using these two approaches simultaneously.…”

Section: Discussionmentioning

confidence: 99%

Identification of Genes and Metabolic Pathways Involved in Resin Yield in Masson Pine by Integrative Analysis of Transcriptome, Proteome and Biochemical Characteristics

Shen

Hou

et al. 2022

IJMS

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Masson pine (Pinus massoniana L.) is one of the most important resin-producing tree species in southern China. However, the molecular regulatory mechanisms of resin yield are still unclear in masson pine. In this study, an integrated analysis of transcriptome, proteome, and biochemical characteristics from needles of masson pine with the high and common resin yield was investigated. The results showed that chlorophyll a (Chl a), chlorophyll b (Chl b), total chlorophyll (Chl C), carotenoids (Car), glucose (Glu), gibberellin A9 (GA9), gibberellin A15 (GA15), and gibberellin A53 (GA53) were significantly increased, whereas fructose (Fru), jasmonic acid (JA), jasmonoyl-L-isoleucine (JA-ILE), gibberellin A1 (GA1), gibberellin A3 (GA3), gibberellin A19 (GA19), and gibberellin A24 (GA24) were significantly decreased in the high resin yield in comparison with those in the common one. The integrated analysis of transcriptome and proteome showed that chlorophyll synthase (chlG), hexokinase (HXK), sucrose synthase (SUS), phosphoglycerate kinase (PGK), dihydrolipoamide dehydrogenase (PDH), dihydrolipoamide succinyltransferase (DLST), 12-oxophytodienoic acid reductase (OPR), and jasmonate O-methyltransferases (JMT) were consistent at the transcriptomic, proteomic, and biochemical levels. The pathways of carbohydrate metabolism, terpenoid biosynthesis, photosynthesis, and hormone biosynthesis may play crucial roles in the regulation of resin yield, and some key genes involved in these pathways may be candidates that influence the resin yield. These results provide insights into the molecular regulatory mechanisms of resin yield and also provide candidate genes that can be applied for the molecular-assisted selection and breeding of high resin-yielding masson pine.

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Section: Discussionmentioning

confidence: 99%

Identification of Genes and Metabolic Pathways Involved in Resin Yield in Masson Pine by Integrative Analysis of Transcriptome, Proteome and Biochemical Characteristics

Shen

Hou

et al. 2022

IJMS

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“…Terpenoid backbone biosynthesis-related proteins have also been reported to be involved in resin yield. Mei et al [ 1 ] revealed that transcript levels of HMGR, MDS, HDS, and IDI showed higher expression in the high resin yielder, which could be used as the critical targets for the molecular-assisted selection of high resin-yielding germplasm in masson pine. In the present study, MDS and SesquiTPS1 were up-regulated, whereas MK and PMK were down-regulated in the high resin yielder, which was consistent with expression trends in needles of high resin-yielding masson pine [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Masson pine ( Pinus massoniana Lamb.) is one of the most important resin-producing conifer species in China, and approximately 90% of the resin is tapped from this species [ 1 ]. Resin, a complex mixture of different monoterpenes, sesquiterpenes, and diterpenes [ 2 , 3 ], is stored in resin ducts from stems, roots, needles, and reproductive structures [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Physiological, Proteomic, and Resin Yield-Related Genes Expression Analysis Provides Insights into the Mechanisms Regulating Resin Yield in Masson Pine

Li,

Zhou,

Hou

et al. 2023

IJMS

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Masson pine (Pinus massoniana Lamb.) is an important resin-producing conifer species in China. Resin yield is a highly heritable trait and varies greatly among different genotypes. However, the mechanisms regulating the resin yield of masson pine remain largely unknown. In this study, physiological, proteomic, and gene expression analysis was performed on xylem tissues of masson pine with high and low resin yield. Physiological investigation showed that the activity of terpene synthase, as well as the contents of soluble sugar, jasmonic acid (JA), methyl jasmonate (MeJA), gibberellins (GA1, GA4, GA9, GA19, and GA20), indole-3-acetic acid (IAA), and abscisic acid (ABA) were significantly increased in the high yielder, whereas sucrose and salicylic acid (SA) were significantly decreased compared with the low one. A total of 2984 differentially expressed proteins (DEPs) were identified in four groups, which were mainly enriched in the biosynthesis of secondary metabolites, protein processing in the endoplasmic reticulum, carbohydrate metabolism, phytohormone biosynthesis, glutathione metabolism, and plant-pathogen interaction. Integrated physiological and proteomic analysis revealed that carbohydrate metabolism, terpenoid biosynthesis, resistance to stress, as well as JA and GA biosynthesis and signaling, play key roles in regulating resin yield. A series of proteins associated with resin yield, e.g., terpene synthase proteins (TPSs), ATP-binding cassette transporters (ABCs), glutathione S-transferase proteins (GSTs), and heat shock proteins (HSPs), were identified. Resin yield-related gene expression was also associated with resin yield. Our study unveils the implicated molecular mechanisms regulating resin yield and is of pivotal significance to breeding strategies of high resin-yielding masson pine cultivars.

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“…In the Diterpenoid biosynthesis pathway, relative to P. massoniana , six LDS genes (transcript_11389, transcript_13308, transcript_14223, transcript_18588, transcript_20969), diterpene synthase (transcript_14223) and delta-selinene synthase (transcript_13308), longifolene synthase (transcript_20969) was up-regulated in P. pygmaea ( Figure 3 ). LDS is the crucial enzyme of (13E)-Labda-7,13-dien-15-ol biosynthesis [ 44 ], LDS was identified one of the candidate genes for screening higher oleoresin yield of P. massoniana [ 45 ]. The high expression of LDS gene may be due to the multi-branching of P. pygmaea .…”

Section: Discussionmentioning

confidence: 99%

Targeted Metabolic and Transcriptomic Analysis of Pinus yunnanensis var. pygmaea with Loss of Apical Dominance

Xiao

Yang

Wang

et al. 2022

CIMB

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Pinus yunnanensis var. pygmaea demonstrates obvious loss of apical dominance, inconspicuous main trunk, which can be used as an ideal material for dwarfing rootstocks. In order to find out the reasons for the lack of apical dominance of P. pygmaea, endogenous phytohormone content determination by liquid chromatography–tandem mass spectrometry (LC–MS/MS) and comparative transcriptomes were performed on the shoot apical meristem and root apical meristem of three pine species (P. massoniana, P. pygmaea, and P. elliottii). The results showed that the lack of CK and the massive accumulation of ABA and GA-related hormones may be the reasons for the loss of shoot apical dominance and the formation of multi-branching, the abnormal synthesis of diterpenoid biosynthesis may lead to the influence of GA-related synthesis, and the high expression of GA 2-oxidase (GA2ox) gene may be the cause of dwarfing. Weighted correlation network analysis (WGCNA) screened some modules that were highly expressed in the shoot apical meristem of P. pygmaea. These findings provided valuable information for identifying the network regulation of shoot apical dominance loss in P. pygmaea and enhanced the understanding of the molecular mechanism of shoot apical dominance growth differences among Pinus species.

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Identification of the diterpenoid biosynthesis genes and their expression status in relation to oleoresin yield of masson pine

Cited by 11 publications

References 58 publications

Identification of Genes and Metabolic Pathways Involved in Resin Yield in Masson Pine by Integrative Analysis of Transcriptome, Proteome and Biochemical Characteristics

Identification of Genes and Metabolic Pathways Involved in Resin Yield in Masson Pine by Integrative Analysis of Transcriptome, Proteome and Biochemical Characteristics

Physiological, Proteomic, and Resin Yield-Related Genes Expression Analysis Provides Insights into the Mechanisms Regulating Resin Yield in Masson Pine

Targeted Metabolic and Transcriptomic Analysis of Pinus yunnanensis var. pygmaea with Loss of Apical Dominance

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