Identification of the elusive peptidergic diuretic hormone in the blood-feeding bug<i>Rhodnius prolixus</i>: a CRF-related peptide

Brugge, Victoria Te; Paluzzi, Jean‐Paul; Schooley, David A.; Orchard, Ian

doi:10.1242/jeb.046292

Cited by 47 publications

(24 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although other DHs are present in insects, the two most studied are calcitonin-like and corticotropin releasing factor-like diuretic hormone (CT/DH and CRF/DH), which both stimulate the secretion of water in the Malpighian tubules (reviewed in [64,65]). Besides diuresis, they appear to have roles in feeding behavior [66,67]. We detected DH peptides in the TG and the brain.…”

Section: Diuretic Hormones -Ion Transport Peptidementioning

confidence: 77%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Abstract. Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/ pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects.

show abstract

Section: Diuretic Hormones -Ion Transport Peptidementioning

confidence: 77%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…The full boxes indicate the CRF-like DH coding region, flanked by dibasic cleavage sites (“(G)KR”). Abbreviations: Apime -DHP, Apis mellifera CRF-like DH precursor [16]; Harsa -DHP, Harpegnathos saltator CRF-like DH precursor (UniProtKB/TrEMBL: E2B7W2); Schgr -DHP, S. gregaria OMP-DH-L precursor (described in this paper); Rhopr -DHP, Rhodnius prolixus CRF-like DH precursor [5], UniProtKB/TrEMBL: F1AYR6; Trica -DHP, Tribolium castaneum CRF-like DH (47) precursor [17]. B) Alignment of the CRF-like peptides and OMPs from Schistocerca gregaria ( Schgr -DH and Schgr -OMP) and Locusta migratoria ( Locmi -CRF/DH and Locmi -OMP).…”

Section: Resultsmentioning

confidence: 99%

“…In many insect species, a diuretic hormone (DH) related to the vertebrate Corticotropin Releasing-Factor (CRF) has been characterized [1]. This CRF-like DH (CRF/DH) was first identified in the moth, Manduca sexta [2], but has now also been isolated and characterized in diverse insect species, such as the beetle Tenebrio molitor [3], the termite Zootermopsis nevadensis [4], the kissing bug Rhodnius prolixus [5] and the locust Locusta migratoria [6], [7]. In addition to its role in stimulating diuresis, the CRF-like DH was also suggested to be involved in the mediation of satiety in L. migratoria , since injection of this locust neuropeptide ( Locmi -CRF/DH) influenced the duration of the meal and the latency to feed [8], [9].…”

Section: Introductionmentioning

confidence: 99%

CRF-Like Diuretic Hormone Negatively Affects Both Feeding and Reproduction in the Desert Locust, Schistocerca gregaria

et al. 2012

View full text Add to dashboard Cite

Diuretic hormones (DH) related to the vertebrate Corticotropin Releasing Factor (CRF) have been identified in diverse insect species. In the migratory locust, Locusta migratoria, the CRF-like DH (CRF/DH) is localized in the same neurosecretory cells as the Ovary Maturating Parsin (OMP), a neurohormone that stimulates oocyte growth, vitellogenesis and hemolymph ecdysteroid levels in adult female locusts. In this study, we investigated whether CRF-like DH can influence feeding and reproduction in the desert locust, Schistocerca gregaria. We identified two highly similar S. gregaria CRF-like DH precursor cDNAs, each of which also encodes an OMP isoform. Alignment with other insect CRF-like DH precursors shows relatively high conservation of the CRF/DH sequence while the precursor region corresponding to OMP is not well conserved. Quantitative real-time RT-PCR revealed that the precursor transcripts mainly occur in the central nervous system and their highest expression level was observed in the brain. Injection of locust CRF/DH caused a significantly reduced food intake, while RNAi knockdown stimulated food intake. Therefore, our data indicate that CRF-like DH induces satiety. Furthermore, injection of CRF/DH in adult females retarded oocyte growth and caused lower ecdysteroid titers in hemolymph and ovaries, while RNAi knockdown resulted in opposite effects. The observed effects of CRF/DH may be part of a wider repertoire of neurohormonal activities, constituting an integrating control system that affects food intake and excretion, as well as anabolic processes like oocyte growth and ecdysteroidogenesis, following a meal. Our discussion about the functional relationship between CRF/DH and OMP led to the hypothesis that OMP may possibly act as a monitoring peptide that can elicit negative feedback effects.

show abstract

“…The gene has now been cloned, but in only a small number of insect species, including D. melanogaster , D. pseudoobscura , T. castaneum , A. pisum , and now R . prolixus (Taylor et al, 2004; Hauser et al, 2008; Weaver and Audsley, 2008; Huybrechts et al, 2010). Recently, the first non-insect arthropod proctolin genes were reported in the Pacific white shrimp, L. vannamei , and the water flea, D. pulex (Ma et al, 2010; Dircksen et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The genes for proctolin and its receptor were, however, eventually identified and cloned in D. melanogaster (Egerod et al, 2003; Johnson et al, 2003a,b; Taylor et al, 2004), although surprisingly, proctolin has not been chemically identified in D. melanogaster (see Nässel and Winther, 2010). Subsequently, the proctolin gene was found in the genomes of only a few insect species, including Tribolium castaneum, Tenebrio molitor , and Acyrthosiphon pisum (Hauser et al, 2008; Li et al, 2008; Weaver and Audsley, 2008; Huybrechts et al, 2010). No proctolin gene has been identified in genomes of Apis mellifera, Aedes aegypti, Bombyx mori, Nasonia vitripennis , or Acromymrex echinatior and three other ant species (see Roller et al, 2008; Hauser et al, 2010; Predel et al, 2010; Nygaard et al, 2011), where proctolin and its receptor are now considered absent.…”

Section: Introductionmentioning

confidence: 99%

The Proctolin Gene and Biological Effects of Proctolin in the Blood-Feeding Bug, Rhodnius prolixus

Orchard¹,

Lee²,

Silva³

et al. 2011

Front. Endocrin.

Self Cite

View full text Add to dashboard Cite

We have reinvestigated the possible presence or absence of the pentapeptide proctolin in Rhodnius prolixus and report here the cloning of the proctolin cDNA. The transcript is expressed in the central nervous system (CNS) and some peripheral tissues. The proctolin prepropeptide encodes a single copy of proctolin along with a possible proctolin-precursor-associated peptide. We have biochemically identified proctolin in CNS extracts and shown its distribution using proctolin-like immunoreactivity. Immunostained processes are found on the salivary glands, female and male reproductive tissues, and heart and associated alary muscles. Proctolin-like immunoreactive bipolar neurons are found on the lateral margins of the common oviduct and bursa. Proctolin is biologically active on R. prolixus tissues, stimulating increases in contraction of anterior midgut and hindgut muscles, and increasing heartbeat frequency. Contrary to the previous suggestion that proctolin is absent from R. prolixus, proctolin is indeed present and biologically active in this medically important bug.

show abstract

Identification of the elusive peptidergic diuretic hormone in the blood-feeding bugRhodnius prolixus: a CRF-related peptide

Cited by 47 publications

References 60 publications

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

CRF-Like Diuretic Hormone Negatively Affects Both Feeding and Reproduction in the Desert Locust, Schistocerca gregaria

The Proctolin Gene and Biological Effects of Proctolin in the Blood-Feeding Bug, Rhodnius prolixus

Contact Info

Product

Resources

About