Identification of the First Nuclear Male Sterility Gene (<i>Male‐sterile 9</i>) in Sorghum

Chen, Junping; Jiao, Yinping; Laza, Haydee; Payton, Paxton; Ware, Doreen; Xin, Zhanguo

doi:10.3835/plantgenome2019.03.0020

Cited by 16 publications

(21 citation statements)

References 45 publications

(76 reference statements)

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“…We have also isolated novel brown midrib (bmr) mutants that can be used to improve digestibility of sorghum stalks, bloomless (blm) mutants that can be used to dissect the mechanisms of drought tolerance and high water use efficiency of sorghum, mutants with altered root morphology, nuclear male-sterile mutants for developing a two-line breeding system, and other mutants that could be useful in future studies (Chen et al 2019a;Jiao et al 2017;Saballos et al 2012;Sattler et al 2014;Scully et al 2016;Tishchenko et al 2020;Xin et al 2017). The mutant library may possess many other valuable mutations that could eventually be identified and exploited to introduce novel traits for genomic studies and breeding.…”

Section: Other Mutantsmentioning

confidence: 99%

Sorghum genetic, genomic, and breeding resources

Xin

Wang²,

Cuevas

et al. 2021

Planta

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Main conclusion Sorghum research has entered an exciting and fruitful era due to the genetic, genomic, and breeding resources that are now available to researchers and plant breeders. Abstract As the world faces the challenges of a rising population and a changing global climate, new agricultural solutions will need to be developed to address the food and fiber needs of the future. To that end, sorghum will be an invaluable crop species as it is a stress-resistant C4 plant that is well adapted for semi-arid and arid regions. Sorghum has already remained as a staple food crop in many parts of Africa and Asia and is critically important for animal feed and niche culinary applications in other regions, such as the United States. In addition, sorghum has begun to be developed into a promising feedstock for forage and bioenergy production. Due to this increasing demand for sorghum and its potential to address these needs, the continuous development of powerful community resources is required. These resources include vast collections of sorghum germplasm, high-quality reference genome sequences, sorghum association panels for genome-wide association studies of traits involved in food and bioenergy production, mutant populations for rapid discovery of causative genes for phenotypes relevant to sorghum improvement, gene expression atlas, and online databases that integrate all resources and provide the sorghum community with tools that can be used in breeding and genomic studies. Used in tandem, these valuable resources will ensure that the rate, quality, and collaborative potential of ongoing sorghum improvement efforts is able to rival that of other major crops.

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Section: Other Mutantsmentioning

confidence: 99%

Sorghum genetic, genomic, and breeding resources

Xin

Wang²,

Cuevas

et al. 2021

Planta

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“…For light microscopy analysis, the panicle, flower, or anther tissues of different development stages were fixed and processed according to the procedures described (Chen et al 2019 ). Briefly, the harvested tissues were fixed immediately in FAA solution (contains 95% ethanol, water, 37% formaldehyde, and acetic acid at a ratio of 50:35:10:5) followed by a series of dehydration processes at room temperature in ethanol of increasing concentrations (30 to 100% with increasing concentration by 10%), and then by 1 h incubation in 100% xylene.…”

Section: Methodsmentioning

confidence: 99%

“…Dehydrated flower tissues were infiltrated, embedded in 100% Paraplast Plus (Sigma-Aldrich) at 56 to 60 °C. The embedded tissues were thinly sectioned (5 to 10 μm) using a Leica RM2125 RTS (Leica Biosystems, Buffalo Grove, IL) manual rotatory microtome, placed on a glass slide, processed, and then stained with 0.1% toluidine blue or 0.01% safranin O solution, and imaged (Chen et al 2019 ). The cross sections were analyzed and photographed on an Olympus BX60 microscope equipped with an Olympus DP 80 digital camera (Olympus, Center Valley, PA, USA).…”

Section: Methodsmentioning

confidence: 99%

Morphological analysis and stage determination of anther development in Sorghum [Sorghum bicolor (L.) Moench]

Laza

Kaur-Kapoor

Xin³

et al. 2022

Planta

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Main Conclusion The characteristics of sorghum anthers at 18 classified developmental stages provide an important reference for future studies on sorghum reproductive biology and abiotic stress tolerance of sorghum pollen. Abstract Sorghum (Sorghum bicolor L. Moench) is the fifth-most important cereal crop in the world. It has relatively high resilience to drought and high temperature stresses during vegetative growing stages comparing to other major cereal crops. However, like other cereal crops, the sensitivity of male organ to heat and drought can severely depress sorghum yield due to reduced fertility and pollination efficiency if the stress occurs at the reproductive stage. Identification of the most vulnerable stages and the genes and genetic networks that differentially regulate the abiotic stress responses during anther development are two critical prerequisites for targeted molecular trait selection and for enhanced environmentally resilient sorghum in breeding using a variety of genetic modification strategies. However, in sorghum, anther developmental stages have not been determined. The distinctive cellular characteristics associated with anther development have not been well examined. Lack of such critical information is a major obstacle in the studies of anther and pollen development in sorghum. In this study, we examined the morphological changes of sorghum anthers at cellular level during entire male organ development processes using a modified high-throughput imaging variable pressure scanning electron microscopy and traditional light microscopy methods. We divided sorghum anther development into 18 distinctive stages and provided detailed description of the morphological changes in sorghum anthers for each stage. The findings of this study will serve as an important reference for future studies focusing on sorghum physiology, reproductive biology, genetics, and genomics.

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“…This demonstrated the power of genomics-based crop breeding approaches for accelerating the development of climate ready improved cultivars. Chen et al [ 120 ] mapped a novel nuclear male sterility mutant ( ms9 ) in sorghum using MutMap and identified the causal mutation for male sterility in Sobic.002G221000 gene (designated as Ms9 ), which encode a plant homeotic domain (PHD)-finger transcription factor critical for tapetum degeneration and pollen formation. The Ms9 gene was the first nuclear male sterility gene identified in sorghum and provided an opportunity to control male sterility for the development of a two-line breeding system for hybrid sorghum.…”

Section: Ngs Based Forward Genetics For Identification and Mappingmentioning

confidence: 99%

Next Generation Sequencing Based Forward Genetic Approaches for Identification and Mapping of Causal Mutations in Crop Plants: A Comprehensive Review

Sahu

Sao

Mondal

et al. 2020

Plants

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The recent advancements in forward genetics have expanded the applications of mutation techniques in advanced genetics and genomics, ahead of direct use in breeding programs. The advent of next-generation sequencing (NGS) has enabled easy identification and mapping of causal mutations within a short period and at relatively low cost. Identifying the genetic mutations and genes that underlie phenotypic changes is essential for understanding a wide variety of biological functions. To accelerate the mutation mapping for crop improvement, several high-throughput and novel NGS based forward genetic approaches have been developed and applied in various crops. These techniques are highly efficient in crop plants, as it is relatively easy to grow and screen thousands of individuals. These approaches have improved the resolution in quantitative trait loci (QTL) position/point mutations and assisted in determining the functional causative variations in genes. To be successful in the interpretation of NGS data, bioinformatics computational methods are critical elements in delivering accurate assembly, alignment, and variant detection. Numerous bioinformatics tools/pipelines have been developed for such analysis. This article intends to review the recent advances in NGS based forward genetic approaches to identify and map the causal mutations in the crop genomes. The article also highlights the available bioinformatics tools/pipelines for reducing the complexity of NGS data and delivering the concluding outcomes.

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Identification of the First Nuclear Male Sterility Gene (Male‐sterile 9) in Sorghum

Cited by 16 publications

References 45 publications

Sorghum genetic, genomic, and breeding resources

Sorghum genetic, genomic, and breeding resources

Morphological analysis and stage determination of anther development in Sorghum [Sorghum bicolor (L.) Moench]

Next Generation Sequencing Based Forward Genetic Approaches for Identification and Mapping of Causal Mutations in Crop Plants: A Comprehensive Review

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