Identification of the Functions and Prognostic Values of RNA Binding Proteins in Bladder Cancer

Abstract

Post-transcriptional regulation plays a leading role in gene regulation and RNA binding proteins (RBPs) are the most important posttranscriptional regulatory protein. RBPs had been found to be abnormally expressed in a variety of tumors and is closely related to its occurrence and progression. However, the exact mechanism of RBPs in bladder cancer (BC) is unknown. We downloaded transcriptomic data of BC from the Cancer Genome Atlas (TCGA) database and used bioinformatics techniques for subsequent analysis. A total of 116 differentially expressed RBPs were selected, among which 61 were up-regulated and 55 were down-regulated. We then identified 12 prognostic RBPs including CTIF, CTU1, DARS2, ENOX1, IGF2BP2, LIN28A, MTG1, NOVA1, PPARGC1B, RBMS3, TDRD1, and ZNF106, and constructed a prognostic risk score model. Based on this model we found that patients in the high-risk group had poorer overall survival (P < 0.001), and the area under the receiver operator characteristic curve for this model was 0.677 for 1 year, 0.697 for 3 years, and 0.709 for 5 years. Next, we drew a nomogram based on the risk score and other clinical variables, which showed better predictive performance. Our findings contribute to a better understanding of the pathogenesis, progression and metastasis of BC. The model of these 12 genes has good predictive value and may have good prospects for improving clinical treatment regimens and patient prognosis.

The role of Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs) as m6A readers in cancer

“…Nonetheless, all three IGF2BPs are highly expressed and function as independent prognostic factors in a variety of cancers, including lung cancer 89 , 90 , liver cancer 91 - 93 , breast cancer 94 , 95 , colorectal cancer 96 - 98 , pancreatic cancer 99 - 101 , prostate cancer 102 , bladder cancer 103 - 105 , thyroid cancer 106 - 108 , gastric cancer 109 , 110 , renal cell carcinoma 111 , ovarian cancer 112 , 113 , esophageal squamous cell carcinoma 114 , 115 , acute myeloid leukemia 116 . In addition to the high expression of the IGF2BPs itself, intercellular communication factors, such as tumor-secreted extracellular vesicles (EVs), can maintain the stability of IGF2BPs in cells to promote the development of cancer.…”

Integrated Analysis and Identification of Critical RNA-Binding Proteins in Bladder Cancer

“…Furthermore, 12 prognostic RBP signatures have been explored, including CTIF, CTU1, DARS2, ENOX1, IGF2BP2, LIN28A, MTG1, NOVA1, PPARGC1B, RBMS3, TDRD1, and ZNF106. Studies found that MTG1, CTU1, and PPARGC1B gene expressions were related to optimistic prognoses in patients with BC, whereas high RBMS3, DARS2, ENOX1, IGF2BP2, ZNF106, CTIF, and NOVA1 gene expressions were related to pessimistic prognoses [ 15 ]. However, the exact and functional role of RBPs in the development and prognosis of BC remain unclear.…”

Assessment of Genetic Susceptibility to Multiple Primary Cancers through Whole-Exome Sequencing in Two Large Multi-Ancestry Studies

“…ZNF106 is an RNA binding protein involved in post-transcriptional regulation and insulin receptor signaling. Although germline variation in ZNF106 has not previously been associated with cancer risk, a recent study found it to be associated with worse urinary bladder cancer survival 32 .…”