Identification of the glycoprotein H gene of murine cytomegalovirus

Xu, Jiake; Dallas, Peter B.; Lyons, Paul; Shellam, Geoffrey; Scalzo, Anthony A.

doi:10.1099/0022-1317-73-7-1849

Cited by 32 publications

(17 citation statements)

References 37 publications

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“…In all human and animal herpesviruses studied to date, homologs of glycoprotein H (gH) and glycoprotein L (gL) have been found (9,14,15,17,18,21,22,26,32,34,35,40,41,43,44). These two envelope glycoproteins, which associate to form a gH-gL complex, have been implicated as key participants in fusion events that are critical to herpesvirus infection.…”

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confidence: 99%

The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

Mori

Akkapaiboon

Yang

et al. 2003

J Virol

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The human herpesvirus 6 (HHV-6) variant A U100 gene encodes the third component of the glycoprotein H (gH)-glycoprotein L (gL)-containing complex. Glycosidase digestion analysis showed that the U100 gene products are glycoproteins consisting of an 80-kDa protein with complex N-linked oligosaccharides and a 74-kDa protein with immature, high-mannose N-linked oligosaccharides. Based on these characteristics, we designated the U100 gene products glycoprotein Q (gQ). Only the 80-kDa form of gQ was coimmunoprecipitated with an anti-gH antibody, suggesting that the 80-kDa protein associates with the gH-gL complex in HHV-6-infected cells. Furthermore, the complex was detected in purified virions, suggesting that it may play an important role in viral entry.

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confidence: 99%

The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

Mori

Akkapaiboon

Yang

et al. 2003

J Virol

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“…In the case of the gH no smaller transcript was found, and we conclude that the protein is encoded by the 5.0 kb transcript. That means that the gH transcript does not terminate at the potential polyadenylation signal sequence immediately downstream of the ORF as proposed by Xu et al (1992) but continues through to the next gene and probably stops after the dUTPase gene.…”

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confidence: 99%

“…Recently, the gH gene of the MCMV strain K181 has been identified and sequenced (Xu et al, 1992). After having characterized and expressed the gB gene (Rapp et al, 1992) we examined the sequence and expression of the gH gene of the MCMV strain Smith with the intention of investigating the MCMV glycoproteins further in view of their usefulness as subunit vaccines.…”

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Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus

Rapp

Lučin

Messerle

et al. 1994

Journal of General Virology

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The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb, which is synthesized late in infection. A recombinant vaccinia virus expressing the MCMV gH open reading frame was constructed (Vac-gH). Anti-MCMV serum precipitated a protein of 87K from Vac-gH-infected cells. Reactivity with a monoclonal antibody showed the identity of the MCMV gH with a 87K envelope glycoprotein described previously by Loh and Qualtiere. Immunization of mice with the Vac-gH recombinant gave rise to an anti-gH serum, which neutralized MCMV without complement in vitro.

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“…Organs from animals from each of these groups were explanted onto MEF monolayers and cocultivated for 60 days or until viral CPE was observed. marker for late gene transcription (63). The gH RNA levels and prevalence were analyzed by RT-PCR amplification of the matched ie-1 cDNA samples with a level of sensitivity similar to that of ie-1 RT-PCR (data not shown).…”

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confidence: 99%

Persisting Murine Cytomegalovirus Can Reactivate and Has Unique Transcriptional Activity in Ocular Tissue

Kercher¹,

Mitchell

2002

J Virol

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Cytomegalovirus (CMV) retinitis is an important ocular complication in human immunodeficiency virusinfected individuals and the leading cause of blindness in those not undergoing highly active antiretroviral therapy. Murine CMV (MCMV) infection of mice has been shown to be a useful small-animal model for the study of CMV pathogenesis in the eye. The purpose of this study was to evaluate CMV persistence in ocular tissue and to determine the potential for reactivation. Following subretinal inoculation of immunocompetent BALB/c mice, tissues were tested for infectious virus by plaque assay and for the presence of viral DNA and RNA by PCR. The latent phase of the infection in mouse tissues was analyzed by plaque assay, PCR, and explantation cocultivation in both immunocompetent and cyclophosphamide-treated mice. The acute phase of the infection was resolved by 2 to 3 weeks postinfection, while viral DNA persisted beyond 12 months. Immediate-early 1 transcripts were detected in 100% of the ocular samples tested, and glycoprotein H transcripts were detected in 86% of the samples, but no difference in viral DNA or RNA levels between immunocompetent and immunosuppressed animals was measured. Irrespective of immune status, no in vivo reactivation was detected; however, reactivated virus was observed in 76 to 82% of the eyes following explantation onto a permissive cell layer. The transcriptional activity and relatively high frequency of explantationinduced reactivation in both immunocompetent and immunosuppressed mice suggest that control of MCMV latency in ocular tissue might involve other regulatory events that are not entirely dependent on intact specific immunity.Cytomegalovirus (CMV) is a ␤-herpesvirus that is fairly ubiquitous in the human population and generally causes mild or subclinical disease in healthy individuals (47). Although the acute replication of CMV is eventually cleared by the host immune system, the virus persists by establishing a lifelong latent infection, defined as the presence of viral DNA without the detection of infectious virus. Human CMV (HCMV) has been shown to establish latency in cells of the monocyte/macrophage lineage including hematopoietic progenitor cells (16,42,56), and there is evidence of persistent HCMV infection in aortic endothelial cells (14,15). The ability of CMV to reactivate from a latent state that is subsequently accompanied by asymptomatic viral shedding can periodically occur in healthy, seropositive individuals; however, the specific cell types from which recurrent virus comes are unknown. A significant amount of CMV morbidity can be attributed to reactivation events that are normally controlled by the immune system, based on the observation that immunosuppressed individuals often suffer from HCMV disease (54).CMV has emerged as an important opportunistic pathogen in immunosuppressed persons, and reactivation of latent virus, which can cause significant morbidity in multiple organs including the eye, is frequently observed (20,48). CMV retinitis is a focal, progress...

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Identification of the glycoprotein H gene of murine cytomegalovirus

Cited by 32 publications

References 37 publications

The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus

Persisting Murine Cytomegalovirus Can Reactivate and Has Unique Transcriptional Activity in Ocular Tissue

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