Identification of the Hydroxamate Siderophore Ferricrocin in Cladosporium cladosporioides

Pourhassan, Nina; Gagnon, René; Wichard, Thomas; Bellenger, Jean‐Philippe

doi:10.1177/1934578x1400900429

Cited by 9 publications

(7 citation statements)

References 14 publications

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“…Whereas, the ICP-MS combined with LC allows the easy quantification of metals (and metal-complexes) at very low concentration, ESI-MS measurements provide additional valuable information for structure elucidation, particularly if MS/MS experiments are applied. Using high-resolution-ESI-MS (HR-ESI-MS), hydroxamate-type ligands have been directly identified in seawater samples (Velasquez et al, 2011) or e.g., in fungi (Lehner et al, 2013;Pourhassan et al, 2014) via their natural iron-isotope signature. This approach was improved by the application of a newly developed MatLab based program for the identification of natural isotope patterns of metal complexes (Baars et al, 2014).…”

Section: Toward the Identification Of Organic Ligands In Marine Dissomentioning

confidence: 99%

Identification of Metallophores and Organic Ligands in the Chemosphere of the Marine Macroalga Ulva (Chlorophyta) and at Land-Sea Interfaces

Wichard

2016

Front. Mar. Sci.

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The roles of organic matter in seawater have often been discussed from the aspect of metal toxicity and bioavailability in seawater. In fact, organic ligands, as part of the organic matter, can work as a trace metal ion buffer system. At the same time, however, the release of well-defined metal chelators as exudates by, for example, marine bacteria is necessary to compete with natural metal complexes and sustain the metal acquisition required for several processes including nitrogen fixation. The identification, isolation, and structure elucidation of chelators is, thus, essential to our understanding of metal stress management in the natural habitat and role of these chelators on cellular process. The isolation of an organic ligand from its chemosphere is a challenging task. The purpose of this paper is, therefore, to give an additional perspective on how the effective application of stable isotope pairs of a metal of interest (both cations and oxoanions) combined with mass spectrometric analyses can pave the way to discovering new organic ligands (i.e., metallophores) and the chelating characteristics of dissolved organic matter (DOM): Pairs of isotopes, such as 54 Fe and 58 Fe (or any other pair of available isotopes of a given metal), can be used to create easily detectable unique isotopic signatures in mass spectra when they are bound by chelators. The identification of organic ligands is outlined for a proposed model system of mutualistic interactions between the green macroalga Ulva (Chlorophyta) and associated bacteria, as well as discussed briefly for DOM along land-sea gradients. Overall, the characterization of a broader spectrum of chelators in aquatic systems will open a new window to decipher the eco-physiological functions of organic ligands as a metal ion buffer and metallophores in metal cycling in marine ecosystems.

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Section: Toward the Identification Of Organic Ligands In Marine Dissomentioning

confidence: 99%

Identification of Metallophores and Organic Ligands in the Chemosphere of the Marine Macroalga Ulva (Chlorophyta) and at Land-Sea Interfaces

Wichard

2016

Front. Mar. Sci.

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“…(Sultana et al 2000) dan mampu menghancurkan reproduksi struktur karat serta menurunkan jumlah spora karat yang berpotensi menginfeksi tanaman baru (Sheta 1996). Pourhassana et al (2014) melaporkan bahwa C. cladosporioides menghasilkan siderofor yang dapat menghambat pertumbuhan cendawan patogen. Harapan di kemudian hari C. cladosporioides dapat dimanfaatkan sebagai agens pengendali untuk menurunkan status penyakit karat putih menjadi penyakit sekunder sehingga tidak diperlukan lagi fungisida sintetik untuk pengendaliannya.…”

Section: Identifikasi Molekulerunclassified

Isolasi dan Identifikasi Mikoparasit Utama Pada Karat Krisan

Yusuf¹,

Nuryani²,

Hanudin³

2016

J.Hort

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Abstrak. Pada beberapa jenis cendawan karat dilaporkan terdapat mikoparasit diantaranya Cladosporium sp. Mikoparasit asal Kebun Percobaan Segunung Balai Penelitian Tanaman Hias (Balithi) yang diduga sebagai Cladosporium menghasilkan penekanan yang tinggi terhadap karat putih asal krisan dengan penekanan mencapai 100%. Penelitian bertujuan mengidentifikasi dan mempelajari karakter morfologi dan molekuler cendawan mikoparasit yang terdapat pada pustul karat krisan asal kebun percobaan Balithi. Penelitian dilakukan di Laboratorium Penyakit Balithi Segunung dan Laboratorium Biologi Lembaga Ilmu Pengetahuan Indonesia (LIPI) Cibinong pada Bulan Januari sampai dengan Desember 2012. Identifikasi secara morfologi dilakukan dengan melihat ciri makroskopis dan mikroskopis cendawan. Identifikasi secara molekuler dilakukan berdasarkan analisis pada sekuen Internal Transcribed Spacer (ITS) ribosomal DNA. Amplifikasi PCR pada ITS menggunakan Primer ITS 4: 5`-- TCC TCC GCT TAT TGA TAT GC – 3` dan ITS 5: 5`--GGA AGT AAA AGT CGT AAC AAG G –3`. Hasil pengamatan makroskopik dan mikroskopik menunjukkan bahwa Isolat 567 yang menginfeksi karat putih pada krisan asal Segunung termasuk ke dalam genus Cladosporium. Hasil analisis secara molekuler Cladosporium itu memiliki homologi 100% dengan Cladosporium cladosporioides strain 1-09 (aksesi no: JF502459) yang berada di genbank.

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“…Cladosporium cladosporioides is a common mold in both indoor and outdoor environments 1 , 2 . This species of dematiaceous hyphomycetes can infect immunocompetent and immunodeficient humans and animals and cause diseases including hemorrhagic pneumonia 3 , pulmonary phaeohyphomycosis 4 , and cutaneous phaeohyphomycosis 5 – 7 .…”

Section: Introductionmentioning

confidence: 99%

Assessment of the pulmonary adaptive immune response to Cladosporium cladosporioides infection using an experimental mouse model

et al. 2021

Sci Rep

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Cladosporium cladosporioides causes asthma and superficial and deep infections, mostly in immunodeficient individuals and animals. This study aimed to investigate whether C. cladosporioides spores can enter the lungs through pulmonary circulation and influence pulmonary immune response. We intravenously injected mice with C. cladosporioides spore suspension and conducted several assays on the lungs. Pulmonary hemorrhage symptoms and congestion were most severe on days 1, 2, and 3 post-inoculation (PI). Extensive inflammatory cell infiltration occurred throughout the period of infection. More spores and hyphae colonizing the lungs were detected on days 1, 2, and 3 PI, and fewer spores and hyphae were observed within 21 d of infection. Numerous macrophages, dendritic cells, and neutrophils were observed on day 5 PI, along with upregulation of CD54, an intercellular adhesion molecule. Th1 and Th2 cells increased after infection; specifically, Th2 cells increased considerably on day 5 PI. These results suggest that days 2 and 5 PI represent the inflammatory peak in the lungs and that the Th2 and Th1 signaling pathways are potentially involved in pulmonary immune responses. In conclusion, the further adaptive immune responses played important roles in establishing effective pulmonary immunity against C. cladosporioides systemic infections based on innate immune responses.

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Identification of the Hydroxamate Siderophore Ferricrocin in Cladosporium cladosporioides

Cited by 9 publications

References 14 publications

Identification of Metallophores and Organic Ligands in the Chemosphere of the Marine Macroalga Ulva (Chlorophyta) and at Land-Sea Interfaces

Identification of Metallophores and Organic Ligands in the Chemosphere of the Marine Macroalga Ulva (Chlorophyta) and at Land-Sea Interfaces

Isolasi dan Identifikasi Mikoparasit Utama Pada Karat Krisan

Assessment of the pulmonary adaptive immune response to Cladosporium cladosporioides infection using an experimental mouse model

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