Identification of the
            <i>Listeria monocytogenes</i>
            virulence factors involved in the CAMP reaction

McKellar, R.C.

doi:10.1111/j.1472-765x.1994.tb00809.x

Cited by 10 publications

(3 citation statements)

References 13 publications

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“…Here, we report the identification of a CAMP cohemolysin as a potential virulence determinant in R. anatipestifer strains. CAMP factors that have been identified include the phospholipase C of Listeria monocytogenes (17), Apx toxins of Actinobacillus pleuropneumoniae (9), and lipases of S. aureus (8). CAMP factors have been shown to be virulence factors (7,12,25).…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of CAMP Cohemolysin as a Potential Virulence Factor of Riemerella anatipestifer

et al. 2002

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Riemerella anatipestifer is responsible for exudative septicemia in ducks. The genetic determinant of the CAMP cohemolysin, cam, from a strain of R. anatipestifer was cloned and expressed in Escherichia coli. Chromosomal DNA from serotype 19 strain 30/90 was used to construct a gene library in pBluescript II SK(؊) vector in E. coli XL-1-Blue strain. The clones containing recombinant plasmids were screened for the CAMP reaction with Staphylococcus aureus. Those that showed cohemolysis were chosen for further analysis by sequencing. One of these clones, JFRA8, was subcloned to identify the smallest possible DNA fragment containing the CAMP cohemolysin determinant, which was located on a 3,566-bp BamHI-BstXI fragment which specified a 1,026-bp open reading frame. Clones containing recombinant plasmids carrying cam obtained by PCR cloning into E. coli M15 strain secreted an active CAMP cohemolysin. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analyses confirmed that the recombinant strain expressed a protein with a molecular mass of 37 kDa and that strains from serotypes 1, 2, 3, 5, 6, and 19 expressed the cohemolysin. The deduced amino acid sequence showed high homology to those of O-sialoglycoprotein endopeptidases. Hydrolysis of radioiodinated glycophorin A confirmed that Cam is a sialoglycoprotease.

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of CAMP Cohemolysin as a Potential Virulence Factor of Riemerella anatipestifer

et al. 2002

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“…Nine cultures of L. monocytogenes, with altered hemolytic properties due to mutations or transposon insertion (8), plus three isolates of L. monocytogenes with weak or absent hemolytic reactions (Murray B and two variants of NCTC 5105 from the culture collections) were among the isolates tested. A further 10 rhamnose-negative L. monocytogenes cultures were also tested.…”

mentioning

confidence: 99%

Simple color tests based on an alanyl peptidase reaction which differentiate Listeria monocytogenes from other Listeria species

Clark

McLaughlin

1997

J Clin Microbiol

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The hydrolysis of DL-alanine-␤-naphthylamide and D-alanine-p-nitroanilide for identification of Listeria spp. has been studied with 227 cultures. All species of Listeria, except L. monocytogenes, hydrolyzed these substrates. The reactions were detected by simple chromogenic reactions and could substitute for the CAMP test. Prior to the early 1980s, human listeriosis was considered of relatively minor concern. However, in the 1980s, the incidence of individual cases of listeriosis increased in several countries, and this, together with a series of foodborne outbreaks, caused Listeria monocytogenes to become of significant public health importance (5). Listeria species are ubiquitous in the environment, and within the genus, L. monocytogenes is the major human pathogen (5). Therefore, it is important that Listeria species be correctly identified. For the identification of species within the genus, phenotopic characters, which rely on the fermentation of sugars, and hemolytic reactions are generally used. This paper reports on two color-based amidase tests, carried out with microtiter plates, which differentiate L. monocytogenes from the remainder of the genus. In this study, 141 cultures of Listeria were obtained from culture collections which comprised the following: The Depart

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“…14 The factors involved in the synergistic lysis of RBC and use of CAMP test for identification of L. monocytogenes and L. ivanovii isolates have been reviewed by McKellar (1994). 15 In present study test CAMP test was used to differentiate Listeria monocytogenes from other Listeria spp.…”

Section: Resultsmentioning

confidence: 99%

Study of listeriosis in spontaneous abortions during preganacy at tertiary care centre

Dawane¹,

Shegakar²

2021

IJMMTD

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Listeriosis is an emerging zoonotic disease. Listeria monocytogenes is an uncommon cause of illness in general population, however, in some high risk groups including neonates, pregnant women, elderly persons, immunosuppressed transplant recipients and others with impaired cell mediated immunity, it is important cause of life threatening bacteremia meningoencephalitis. Listeriosis is 18 times more common in pregnancy (12/100,000) than in the non-pregnant population (0.7/100,000) and 16-27% of all infections with Listeria occur in pregnant women. Isolation of bacteria by conventional methods of microscopy and culture on selective agar PALCUM agar after enrichment of samples by using UVM1, UVM2 .Speciation of listeria done by carbohydrate fermentation test followed by study of pathogenicity by hemolysis on sheep blood agar and CAMP test followed by antimicrobial susceptibility test done.Out of total 131 patients having history of Spontaneous abortion were screened for Listeria, in that 10(7.6%) were carrying Listeria out of which 3(2.29%) of them found to be positive for L. monocytogenes and 07(5.34%) were other Listeria spp. The prevalence of L. monocytogenes in present study was 03(2.29%) from spontaneous abortion cases.Based on the results of present study it is concluded that Listeria monocytogenes is responsible for spontaneous abortions in spontaneous abortion during pregnancy in humans.

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Identification of the Listeria monocytogenes virulence factors involved in the CAMP reaction

Cited by 10 publications

References 13 publications

Identification and Characterization of CAMP Cohemolysin as a Potential Virulence Factor of Riemerella anatipestifer

Identification and Characterization of CAMP Cohemolysin as a Potential Virulence Factor of Riemerella anatipestifer

Simple color tests based on an alanyl peptidase reaction which differentiate Listeria monocytogenes from other Listeria species

Study of listeriosis in spontaneous abortions during preganacy at tertiary care centre

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