2006
DOI: 10.1021/bi0600796
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Identification of the Interaction Domain of the Small Terminase Subunit pUL89 with the Large Subunit pUL56 of Human Cytomegalovirus

Abstract: The small terminase subunit pUL89 of human cytomegalovirus (HCMV) is thought to be required for cleavage of viral DNA into unit-length genomes in the cleavage/packaging process. Immunoprecipitations with a UL89-specific antibody demonstrated that pUL89 occurs predominantly as a monomer of approximate M(r) 75.000 together with a dimer of approximate 150.000. This was confirmed by gel permeation chromatography. In view of its putative function, pUL89 needs to be transported into the nucleus. By use of laser scan… Show more

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Cited by 46 publications
(50 citation statements)
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“…In addition, direct binding of pUL51 to both pUL56 and pUL89, as well as of pUL56 to pUL89, was disclosed. The latter has been reported before, based on glutathione S-transferase (GST) pulldown and IP assays with lysates of infected cells (10,47). Importantly, on top of that, our data now indicate a conjoint cooperation among the HCMV terminase proteins for holoenzyme formation, because the pairwise expressed terminase constituents did not interact efficiently until the respective third subunit was present.…”
Section: Discussionsupporting
confidence: 83%
See 1 more Smart Citation
“…In addition, direct binding of pUL51 to both pUL56 and pUL89, as well as of pUL56 to pUL89, was disclosed. The latter has been reported before, based on glutathione S-transferase (GST) pulldown and IP assays with lysates of infected cells (10,47). Importantly, on top of that, our data now indicate a conjoint cooperation among the HCMV terminase proteins for holoenzyme formation, because the pairwise expressed terminase constituents did not interact efficiently until the respective third subunit was present.…”
Section: Discussionsupporting
confidence: 83%
“…1A, construct 2), the first 1,928 nucleotides (out of 2,553) of the UL56 ORF were deleted, and for the UL89 knockout genome (Fig. 1A, construct 3), most of exon 2 (1,049 nucleotides out of 1,137) was removed, including the sequences encoding the proposed interaction domain for pUL56 (6,47). Care was taken to retain putative regulatory sequences of neighboring essential ORFs.…”
Section: Resultsmentioning
confidence: 99%
“…The HCMV terminase responsible for cleavage of concatemeric DNA was shown to consist of two essential proteins, pUL56 and pUL89 (31,36). pUL56 binds to viral capsids as well as to the packaging signal located in the a-repeat of the HCMV genome and has been shown to possess ATPase activity.…”
mentioning
confidence: 99%
“…The pUL33 component may act as an essential accessory protein involved in the formation and/or stability of the terminase complex (7). Homologous terminase components have also been identified for HCMV (pUL89, pUL56, and pUL51) (16)(17)(18)(19)(20)(21)(26)(27)(28) and VZV (pORF45/42, pORF30, and …”
mentioning
confidence: 99%
“…The protein complex that docks or interacts with the capsid portal protein consists of the heterotrimeric viral terminase. Terminases of DNA bacteriophages have been well described (2,3), and those of several members of the Herpesviridae, including herpes simplex virus 1 (HSV-1) (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), human cytomegalovirus (HCMV) (15)(16)(17)(18)(19)(20)(21), Epstein-Barr virus (EBV) (22), and varicella-zoster virus (VZV) (23)(24)(25), are under investigation.…”
mentioning
confidence: 99%